Plasmid name: pBI-1 Size: 9492 bp Resistance: Ampicillin constructed by: Udo Baron Host strain: DH5 alpha, E.coli "sure" Reference: Baron, Udo et al. (1995),Nucleic Acids Res.,17, 3605-3606
The plasmid consists out of four main fragments:
Afl II c/ttaag Not I gc/ggccgc Sap I gcttcttc 1/4 Age I a/ccggt Nru I tcg/cga Sfi I ggccnnnn/nggcc Avr II c/ctagg Nsi I atgca/t SnaB I tac/gta Eag I c/ggccg PaeR7 I c/tcgag Sse8337 I cctgca/gg Fse I ggccgg/cc Pml I cac/gtg Swa I attt/aaat Hind III a/agctt Rsr II cg/gwccg Tth111 I gacn/nngtc Nhe I g/ctagc Sal I g/tcgac Xho I c/tcgag
Enzyme Position Pst I ctgca/g 1 Stu I agg/cct 457 Cla I at/cgat 1557 EcoR V gat/atc 1845 Dra III cacnnn/gtg 1919 Bcl I t/gatca 2080 Eco47 III agc/gct 2567 Acc I gt/mkac 3497 Sna I gta/tac 3497 Xca I gta/tac 3497 Esp I gc/tnagc 3746 Apa I gggcc/c 4609 Bsp120 I g/ggccc 4609 Msc I tgg/cca 4752 Bgl II a/gatct 4783 Bsa I ggtctc 1/5 6367 PshA I gacnn/nngtc 7295 Nae I gcc/ggc 7336 Spe I a/ctagt 7765 EcoN I cctnn/nnnagg 7861 SgrA I cr/ccggyg 8050 Pac I ttaat/taa 8157 Sph I gcatg/c 8820 BsaA I yac/gtr 9327
Enzyme Sites Positions Aat II gacgt/c 2 1353 7218 Ase I at/taat 2 5236 6471 Asp718 g/gtacc 2 425 582 Bbe I ggcgc/c 2 7412 9447 BsaB I gatnn/nnatc 2 2061 7517 BspE I t/ccgga 2 8268 8784 BstB I tt/cgaa 2 8523 9311 BstE II g/gtnacc 2 7379 8871 Drd I gacnnnn/nngtc 2 2837 5509 Ehe I ggc/gcc 2 7412 9447 Fsp I tgc/gca 2 874 6520 Kas I g/gcgcc 2 7412 9447 Kpn I ggtac/c 2 425 582 Nar I gg/cgcc 2 7412 9447 Nco I c/catgg 2 4198 9481 Sac II ccgc/gg 2 17 560 Sca I agt/act 2 4360 6778 Spl I c/gtacg 2 3505 9325 Sty I c/cwwgg 2 4198 9481 Xmn I gaann/nnttc 2 3817 6895 Bsm I gaatgc 1/-1 3 7359 7604 7703 BspM I acctgc 4/8 3 1517 3389 8072 BssH II g/cgcgc 3 2232 7355 7458 BstX I ccannnnn/ntgg 3 2947 3564 4755 Bsu36 I cc/tnagg 3 957 4712 8866 EcoR I g/aattc 3 7 570 4703 Hpa I gtt/aac 3 1158 1782 7620 Mlu I a/cgcgt 3 2036 2816 3241 PflM I ccannnn/ntgg 3 1973 2339 4983 PpuM I rg/gwccy 3 4091 7469 8300 Sma I ccc/ggg 3 429 586 608 Xba I t/ctaga 3 597 4062 7759 Xcm I ccannnnn/nnnntgg 3 2944 3689 8742 Xma I c/ccggg 3 429 586 608
Aat II 2 BssH II 3 Hga I 21 Pml I - Acc I 1 BstB I 2 HgiA I 11 PpuM I 3 Afl II - BstE II 2 Hha I 53 PshA I 1 Afl III 7 BstK I 45 Hinc II 7 Pst I 1 Age I - BstN I 23 Hind III - Pvu I 4 Aha II 11 BstU I 48 Hinf I 20 Pvu II 4 Alu I 30 BstX I 3 HinP I 53 Rma I 12 Alw I 32 BstY I 16 Hpa I 3 Rsa I 17 AlwN I 5 Bsu36 I 3 Hpa II 46 Rsr II - Apa I 1 Cfr10 I 9 Hph I 17 Sac I 5 ApaL I 4 Cla I 1 Kas I 2 Sac II 2 Ase I 2 Csp6 I 17 Kpn I 2 Sal I - Asp718 2 Dde I 16 Mae II 18 Sap I - Ava I 7 Dpn I 45 Mae III 36 Sau3A I 45 Ava II 10 Dpn II 45 Mbo I 45 Sau96 I 24 Avr II - Dra I 7 Mbo II 30 Sca I 2 BamH I 4 Dra III 1 Mcr I 14 ScrF I 45 Ban I 9 Drd I 2 Mlu I 3 SfaN I 17 Ban II 8 Dsa I 9 Mme I 5 Sfe I 7 Bbe I 2 Dsa V 45 Mnl I 59 Sfi I - Bbs I 8 Eae I 6 Msc I 1 SgrA I 1 Bbv I 29 Eag I - Mse I 32 Sma I 3 BceF I 17 Ear I 8 Msp I 46 Sna I 1 Bcl I 1 Ecl136 I 5 Nae I 1 SnaB I - Bcn I 22 Eco47 III 1 Nar I 2 Spe I 1 Bgl I 6 Eco57 I 7 Nci I 22 Sph I 1 Bgl II 1 EcoN I 1 Nco I 2 Spl I 2 Bsa I 1 EcoO109 I 5 Nde I 5 Sse8337 I - BsaA I 1 EcoR I 3 Nhe I - Ssp I 4 BsaB I 2 EcoR II 23 Nla III 32 Stu I 1 BsaJ I 26 EcoR V 1 Nla IV 35 Sty I 2 Bsg I 7 Ehe I 2 Not I - Swa I - BsiE I 14 Esp I 1 Nru I - Taq I 33 BsiY I 21 Fau I 15 Nsi I - Tfi I 10 Bsm I 3 Fse I - Nsp I 5 Tth111 I - BsmA I 11 Fnu4H I 56 Nsp7524 I 5 Tth111 II 6 Bsp120 I 1 Fok I 27 NspB II 19 Xba I 3 Bsp1286 I 15 Fsp I 2 NspC I 5 Xca I 1 BspE I 2 Gdi II 5 Pac I 1 Xcm I 3 BspH I 5 Gsu I 9 PaeR7 I - Xho I - BspM I 3 Hae I 10 PflM I 3 Xma I 3 BspW I 41 Hae II 13 Ple I 10 Xmn I 2 Bsr I 25 Hae III 34
1 80 CTGCAGGAATTCGGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCC CTGCAGGAATTCGGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCC AGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTG AAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAA AGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGA GAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGA TAGAGAAAAGTGAAAGTCGAGCTCGGTACCCGGGTCGAGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTT TAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTC CGCGGCCCCGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGGATCCCCGGGATCGAAAGAGCCTGCTAAAGCAAAAAA GAAGTCACCATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGTCTGGACACCAG CAAGGAGCTGCTCAAGCGCGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAAACCCTGGCGTTACCCAACTTAATCGC CTTGCAGCACATCCCCCTTTCGCCAGCTGGCTTTATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAG CCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTG AGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTAACCTATTCC ATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTG GCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTTGGCGTTTCATCTGTGGTGCAACGTGCGCTGGGTCG GTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTG ATGGTGCTGCGTTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTC GTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGAAC TGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAG GTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCT GAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACG GCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGC AAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGT GCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACA CGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCTGCTG ACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGT GATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATC CTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTG GATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCT GATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATC CCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCG GCTTACGGCGGTGATTTTGGCGATACGCCGAACCATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCC GCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCA GCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGT GAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCA ACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGGATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGT GGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGAT TTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGCTGTGGATTGGCGATAA AAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCC GCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACG GCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCG GAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGG CGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTAT CCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAG CGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCA GCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATC GACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCG CTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGCAGGCCATGTCTGAAAGTATTCGCGTAAGGAAATCC ATTATGTACTATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATGGGAGCCTA CTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATGAGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTT CTCTGTTGTCGCTATTATTCCAACCGCTGTTGGTCTGCTTTCTGACAAACTCGGCCTCGACTCTAGACTGAGAACTTCAG GGTGAGTTTGGGGACCCTTGATTGTTCTTTCTTTTTCGCTATTGAAAAATTCATGTTATATGGAGGGGGCAAAGTTTTCA GGGTGTTGTTTAGAATGGGAAGATGTCCCTTGTATCACCATGGACCCTCATGATAATTTTGTTTCTTTCACTTTCTACTC TGTTGACAACCATTGTCTCCTCTTATTTTCTTTTCATTTTCTGTAACTTTTTTCGTTAAACTTTAGCTTGCATTTGTAAC GAATTTTTAAATTCACTTTCGTTTATTTGTCAGATTGTAAGTACTTTCTCTAATCACTTTTTTTTCAAGGCAATCAGGGT AATTATATTGTACTTCAGCACAGTTTTAGAGAACAATTGTTATAATTAAATGATAAGGTAGAATATTTCTGCATATAAAT TCTGGCTGGCGTGGAAATATTCTTATTGGTAGAAACAACTACATCCTGGTAATCATCCTGCCTTTCTCTTTATGGTTACA ATGATATACACTGTTTGAGATGAGGATAAAATACTCTGAGTCCAAACCGGGCCCCTCTGCTAACCATGTTCATGCCTTCT TCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTGTTGTGCTGTCTCATCATTTTGGCAAAGAATTCACTCCTCAGGTG CAGGCTGCCTATCAGAAGGTGGTGGCTGGTGTGGCCAATGCCCTGGCTCACAAATACCACTGAGATCTTTTTCCCTCTGC CAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGGTAATAAAGGAAATTTATTTTCATTGCAATAG TGTGTGGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCATTTAAAACATCAGAATGAGTATTTG GTTTAGAGTTTGGCAACATATGCCATATGCTGGCTGCCATGAACAAAGGTGGCTATAAAGAGGTCATCAGTATATGAAAC AGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTGACTTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTA TTTTTTTCTTTAACATCCCTAAAATTTTCCTTACATGTTTTACTAGCCAGATTTTTCCTCCTCTCCTGACTACTCCCAGT CATAGCTGTCCCTCTTCTCTTATGAACTCGACTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTG GGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTA TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACG GGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT CCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAA TCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACG ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGC AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTT GCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCA CGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAA AAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAG CACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGA GAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAAC CCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAAT GCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTA TCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTC CCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCC TTTCGTCTTCACTCGACGCTGGTCGAGCTGATACTTCCCGTCCGCCAGGGGACATGCCGGCGATGCTGAAGGTCGCGCGC ATTCCCGATGAAGAGGCCGGTTACCGCCTGTTGACCTGGTGGGACGGGCAGGGCGCCGCCCGAGTCTTGCCTCGGCGGCG GGCGCTCTGCTCATGGAGCGCGCGTCCGGGGACCTTGCACAGATAGCGTGGTCCGGCCAGACGACGAGGCTTGCAGGATC ATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAA AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCA CAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCCTC TAGAACTAGTGGATCCCCCCTAGAGTATTACAATAGCTAAGAATTTCGTCATCGCTGAATACAGTTACATTTTACAATTT GGACTTTCCGCCCTTCTTGGCCTTTATGAGGATCTCTCTGATTTTTCTTGCGTCGAGTTTTCCGGTAAGACCTTTCGGTA CTTCGTCCACAAACACAACTCCTCCGCGCAACTTTTTGGCGGTTGTTACTTGACTGGCGACGTAATCCACGATCTCTTTT TCCGTCATCGTCTTTCCGTGCTCCAAAACAACAACGGCGGCGGGAAGTTCACCGGCGTCATCGTCGGGAAGACCTGCCAC GCCCGCGTCGAAGATGTTGGGGTGTTGTAACAATATCGACTCCAATTCAGCGGGGGCCACCTGGTATCCTTTGTATTTAA TTAAAGACTTCAAGCGGTCAACTATGAAGAAGTGTTCGTCTTCGTCCCAGTAAGCTATGTCTCCAGAATGTAGCCATCCA TCCTTGTCAATCAAGGCGTTGGTCGCTTCCGGATTGTTTACATAACCGGACATAATCATAGGTCCTCTGACACATAATTC GCCTCTCTGATTAACGCCCAGCGTTTTCCCGGTATCCAGATCCACAACCTTCGCTTCAAAAAATGGAACAACTTTACCGA CCGCGCCCGGTTTATCATCCCCCTCGGGTGTAATCAGAATAGCTGATGTAGTCTCAGTGAGCCCATATCCTTGTCGTATC CCTGGAAGATGGAAGCGTTTTGCAACCGCTTCCCCGACTTCTTTCGAAAGAGGTGCGCCCCCAGAAGCAATTTCGTGTAA ATTAGATAAATCGTATTTGTCAATCAGAGTGCTTTTGGCGAAGAATGAAAATAGGGTTGGTACTAGCAACGCACTTTGAA TTTTGTAATCCTGAAGGGATCGTAAAAACAGCTCTTCTTCAAATCTATACATTAAGACGACTCGAAATCCACATATCAAA TATCCGAGTGTAGTAAACATTCCAAAACCGTGATGGAATGGAACAACACTTAAAATCGCAGTATCCGGAATGATTTGATT GCCAAAAATAGGATCTCTGGCATGCGAGAATCTGACGCAGGCAGTTCTATGCGGAAGGGCCACACCCTTAGGTAACCCAG TAGATCCAGAGGAGTTCATTATCAGTGCAATTGTTTTGTCACGATCAAAGGACTCTGGTACAAAATCGTATTCATTAAAA CCGGGAGGTAGATGAGATGTGACGAACGTGTACATCGACTGAAATCCCTGGTAATCCGTTTTAGAATCCATGATAATAAT TTTCTGGATTATTGGTAATTTTTTTTGCACGTTCAAAATTTTTTGCAACCCCTTTTTGGAAACAAACACTACGGTAGGCT GCGAAATGTTCATACTGTTGAGCAATTCACGTTCATTATAAATGTCGTTCGCGGGCGCAACTGCAACTCCGATAAATAAC GCGCCCAACACCGGCATAAAGAATTGAAGAGAGTTTTCACTGCATACGACGATTCTGTGATTTGTATTCAGCCCATATCG TTTCATAGCTTCTGCCAACCGAACGGACATTTCGAAGTATTCCGCGTACGTGATGTTCACCTCGATATGTGCATCTGTAA AAGCAATTGTTCCAGGAACCAGGGCGTATCTCTTCATAGCCTTATGCAGTTGCTCTCCAGCGGTTCCATCCTCTAGCGGA TAGAATGGCGCCGGGCCTTTCTTTATGTTTTTGGCGTCTTCCATGGTACGGG