Plasmid name: pBI-3 Size: 7727 bp
Resistance: Ampicillin constructed by: Udo Baron, Rolf Sprengel
Host strain: DH5 alpha, E.coli "sure"
Reference: Baron, Udo et al. (1995),Nucleic Acids Res.,17, 3605-3606
The plasmid consists out of four main fragments:
Afl II c/ttaag Nhe I g/ctagc SgrA I cr/ccggyg Age I a/ccggt Nru I tcg/cga SnaB I tac/gta Avr II c/ctagg Nsi I atgca/t Spe I a/ctagt BsaA I yac/gtr Pac I ttaat/taa Sph I gcatg/c BspE I t/ccgga PaeR7 I c/tcgag Sse8337 I cctgca/gg BstB I tt/cgaa Pml I cac/gtg Swa I attt/aaat EcoN I cctnn/nnnagg Rsr II cg/gwccg Tth111 I gacn/nngtc Fse I ggccgg/cc Sap I gcttcttc 1/4 Xho I c/tcgag Hind III a/agctt Sfi I ggccnnnn/nggcc
Enzyme Position Pst I ctgca/g 1 Stu I agg/cct 457 Cla I at/cgat 1502 EcoR V gat/atc 1790 Dra III cacnnn/gtg 1864 Bcl I t/gatca 2025 Eco47 III agc/gct 2512 Sna I gta/tac 3442 Xca I gta/tac 3442 Spl I c/gtacg 3450 Esp I gc/tnagc 3691 Msc I tgg/cca 4697 Bgl II a/gatct 4728 Bsa I ggtctc 1/5 6312 PshA I gacnn/nngtc 7240 Nae I gcc/ggc 7281 BstE II g/gtnacc 7324 Bbe I ggcgc/c 7357 Ehe I ggc/gcc 7357 Kas I g/gcgcc 7357 Nar I gg/cgcc 7357 Sal I g/tcgac 7715 Not I gc/ggccgc 7721 Eag I c/ggccg 7722
Enzyme Sites Positions Aat II gacgt/c 2 1298 7163 Acc I gt/mkac 2 3442 7715 Apa I gggcc/c 2 650 4554 Ase I at/taat 2 5181 6416 Asp718 g/gtacc 2 425 582 BsaB I gatnn/nnatc 2 2006 7462 Bsp120 I g/ggccc 2 650 4554 BspM I acctgc 4/8 2 1462 3334 Bsu36 I cc/tnagg 2 902 4657 Drd I gacnnnn/nngtc 2 2782 5454 Fsp I tgc/gca 2 819 6465 Kpn I ggtac/c 2 425 582 Nco I c/catgg 2 645 4143 PpuM I rg/gwccy 2 4036 7414 Sac II ccgc/gg 2 17 560 Sca I agt/act 2 4305 6723 Sma I ccc/ggg 2 429 586 Sty I c/cwwgg 2 645 4143 Xcm I ccannnnn/nnnntgg 2 2889 3634 Xma I c/ccggg 2 429 586 Xmn I gaann/nnttc 2 3762 6840 Bsm I gaatgc 1/-1 3 7304 7549 7648 BssH II g/cgcgc 3 2177 7300 7403 BstX I ccannnnn/ntgg 3 2892 3509 4700 Hpa I gtt/aac 3 1103 1727 7565 Mlu I a/cgcgt 3 1981 2761 3186 PflM I ccannnn/ntgg 3 1918 2284 4928 Xba I t/ctaga 3 597 4007 7704
Aat II 2 BssH II 3 Hga I 17 Pml I - Acc I 2 BstB I - HgiA I 10 PpuM I 2 Afl II - BstE II 1 Hha I 46 PshA I 1 Afl III 6 BstK I 35 Hinc II 7 Pst I 1 Age I - BstN I 18 Hind III - Pvu I 5 Aha II 8 BstU I 42 Hinf I 15 Pvu II 4 Alu I 24 BstX I 3 HinP I 46 Rma I 9 Alw I 27 BstY I 13 Hpa I 3 Rsa I 12 AlwN I 5 Bsu36 I 2 Hpa II 35 Rsr II - Apa I 2 Cfr10 I 6 Hph I 14 Sac I 5 ApaL I 4 Cla I 1 Kas I 1 Sac II 2 Ase I 2 Csp6 I 12 Kpn I 2 Sal I 1 Asp718 2 Dde I 14 Mae II 11 Sap I - Ava I 5 Dpn I 38 Mae III 29 Sau3A I 38 Ava II 9 Dpn II 38 Mbo I 38 Sau96 I 22 Avr II - Dra I 7 Mbo II 20 Sca I 2 BamH I 5 Dra III 1 Mcr I 15 ScrF I 35 Ban I 8 Drd I 2 Mlu I 3 SfaN I 16 Ban II 8 Dsa I 9 Mme I 5 Sfe I 7 Bbe I 1 Dsa V 35 Mnl I 47 Sfi I - Bbs I 5 Eae I 7 Msc I 1 SgrA I - Bbv I 27 Eag I 1 Mse I 26 Sma I 2 BceF I 16 Ear I 5 Msp I 35 Sna I 1 Bcl I 1 Ecl136 I 5 Nae I 1 SnaB I - Bcn I 17 Eco47 III 1 Nar I 1 Spe I - Bgl I 6 Eco57 I 6 Nci I 17 Sph I - Bgl II 1 EcoN I - Nco I 2 Spl I 1 Bsa I 1 EcoO109 I 5 Nde I 5 Sse8337 I - BsaA I - EcoR I 4 Nhe I - Ssp I 4 BsaB I 2 EcoR II 18 Nla III 30 Stu I 1 BsaJ I 21 EcoR V 1 Nla IV 34 Sty I 2 Bsg I 7 Ehe I 1 Not I 1 Swa I - BsiE I 15 Esp I 1 Nru I - Taq I 27 BsiY I 18 Fau I 11 Nsi I - Tfi I 7 Bsm I 3 Fse I - Nsp I 4 Tth111 I - BsmA I 10 Fnu4H I 55 Nsp7524 I 4 Tth111 II 4 Bsp120 I 2 Fok I 23 NspB II 18 Xba I 3 Bsp1286 I 14 Fsp I 2 NspC I 4 Xca I 1 BspE I - Gdi II 6 Pac I - Xcm I 2 BspH I 5 Gsu I 7 PaeR7 I - Xho I - BspM I 2 Hae I 9 PflM I 3 Xma I 2 BspW I 39 Hae II 13 Ple I 8 Xmn I 2 Bsr I 23 Hae III 32
1 80 CTGCAGGAATTCGGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCC CTGCAGGAATTCGGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCC AGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTG AAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAA AGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGA GAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGA TAGAGAAAAGTGAAAGTCGAGCTCGGTACCCGGGTCGAGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTT TAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTC CGCGGCCCCGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGATCGACGGATCCGGGGAATTCCCCAGTCTCAGGA TCCACCATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAA CCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCG ATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAA AGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCC CATCTACACCAACGTAACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGC TCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCAT CTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACG CGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGTTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGA TGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTT AATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGT TTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTT ATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCG GTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGA AAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTC AGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCAT TATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCA CGGCATGGTGCCAATGAATCTGCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGC AGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTG TATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATAT TATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTT CGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAA TACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATA TGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACCATCGCCAGTTCTGTATGAACG GTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTA TCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCT GGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAAC TACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGGATGGTCAGAAGCC GGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCC GCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTC TTTCACAGCTGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAAC GACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGC CGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATC AGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAA GTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCT CGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACA TGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGG CGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGC GGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGG CGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGCAGGCCATGTC TGAAAGTATTCGCGTAAGGAAATCCATTATGTACTATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCT TTTACTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATGAGCAAAAGTGAT ACGGGTATTATTTTTGCCGCTATTTCTCTGTTGTCGCTATTATTCCAACCGCTGTTGGTCTGCTTTCTGACAAACTCGGC CTCGACTCTAGACTGAGAACTTCAGGGTGAGTTTGGGGACCCTTGATTGTTCTTTCTTTTTCGCTATTGAAAAATTCATG TTATATGGAGGGGGCAAAGTTTTCAGGGTGTTGTTTAGAATGGGAAGATGTCCCTTGTATCACCATGGACCCTCATGATA ATTTTGTTTCTTTCACTTTCTACTCTGTTGACAACCATTGTCTCCTCTTATTTTCTTTTCATTTTCTGTAACTTTTTTCG TTAAACTTTAGCTTGCATTTGTAACGAATTTTTAAATTCACTTTCGTTTATTTGTCAGATTGTAAGTACTTTCTCTAATC ACTTTTTTTTCAAGGCAATCAGGGTAATTATATTGTACTTCAGCACAGTTTTAGAGAACAATTGTTATAATTAAATGATA AGGTAGAATATTTCTGCATATAAATTCTGGCTGGCGTGGAAATATTCTTATTGGTAGAAACAACTACATCCTGGTAATCA TCCTGCCTTTCTCTTTATGGTTACAATGATATACACTGTTTGAGATGAGGATAAAATACTCTGAGTCCAAACCGGGCCCC TCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTGTTGTGCTGTCTCATCATTT TGGCAAAGAATTCACTCCTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCTGGTGTGGCCAATGCCCTGGCTCACAAAT ACCACTGAGATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGGTAATAA AGGAAATTTATTTTCATTGCAATAGTGTGTGGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCA TTTAAAACATCAGAATGAGTATTTGGTTTAGAGTTTGGCAACATATGCCATATGCTGGCTGCCATGAACAAAGGTGGCTA TAAAGAGGTCATCAGTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTGACTTGAGGTTAGAT TTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAACATCCCTAAAATTTTCCTTACATGTTTTACTAGCCAGATTTT TCCTCCTCTCCTGACTACTCCCAGTCATAGCTGTCCCTCTTCTCTTATGAACTCGACTGCATTAATGAATCGGCCAACGC GCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCG GCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAG CATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAG CTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC TTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCC GTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGC AGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA TCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCA AGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT TGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTG ACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCAC GCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCC GCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGC CATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAG TTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCA GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGG TGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATA CCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCG CTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGG GTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCC TTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAA CAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTA TAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCACTCGACGCTGGTCGAGCTGATACTTCCCGTCCGCCAGGGGACAT GCCGGCGATGCTGAAGGTCGCGCGCATTCCCGATGAAGAGGCCGGTTACCGCCTGTTGACCTGGTGGGACGGGCAGGGCG CCGCCCGAGTCTTGCCTCGGCGGCGGGCGCTCTGCTCATGGAGCGCGCGTCCGGGGACCTTGCACAGATAGCGTGGTCCG GCCAGACGACGAGGCTTGCAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCAC ACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAA TAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAA TGTATCTTATCATGTCTGGATCCTCTAGAACTAGGTCGACGCGGCCG