Plasmid name: pUHD 10-3 Size: 3146 bp Resistance: Ampicillin constructed by: Manfred Gossen Host strain: E.coli "sure" Reference: Manfred Gossen unpublished
The plasmid consists of four main fragments:
Acc I gt/mkac Eco47 III agc/gct Pst I ctgca/g
Afl II c/ttaag EcoN I cctnn/nnnagg Rsr II cg/gwccg
Age I a/ccggt EcoR V gat/atc Sal I g/tcgac
Apa I gggcc/c Esp I gc/tnagc Sap I gcttcttc 1/4
Avr II c/ctagg Fse I ggccgg/cc Sfi I ggccnnnn/nggcc
Bcl I t/gatca Mlu I a/cgcgt SgrA I cr/ccggyg
Bgl II a/gatct Msc I tgg/cca Sna I gta/tac
BsaA I yac/gtr Nco I c/catgg SnaB I tac/gta
Bsg I gtgcag 16/14 Nde I ca/tatg Spe I a/ctagt
Bsp120 I g/ggccc Nhe I g/ctagc Sph I gcatg/c
BspE I t/ccgga Not I gc/ggccgc Spl I c/gtacg
BspM I acctgc 4/8 Nru I tcg/cga Sse8337 I cctgca/gg
BstB I tt/cgaa Nsi I atgca/t Sty I c/cwwgg
BstX I ccannnnn/ntgg Pac I ttaat/taa Swa I attt/aaat
Bsu36 I cc/tnagg PflM I ccannnn/ntgg Tth111 I gacn/nngtc
Cla I at/cgat Pml I cac/gtg Xca I gta/tac
Dra III cacnnn/gtg PshA I gacnn/nngtc Xcm I ccannnnn/nnnntgg
Eag I c/ggccg
Enzyme Position PaeR7 I c/tcgag 1 Xho I c/tcgag 1 Stu I agg/cct 336 Bbs I gaagac 2/6 414 Dsa I c/crygg 439 Sac II ccgc/gg 439 EcoR I g/aattc 449 Xba I t/ctaga 476 Hpa I gtt/aac 615 BsaB I gatnn/nnatc 714 PpuM I rg/gwccy 765 Bbe I ggcgc/c 823 Ehe I ggc/gcc 823 Kas I g/gcgcc 823 Nar I gg/cgcc 823 BstE II g/gtnacc 855 Nae I gcc/ggc 899 Hind III a/agctt 940 Pvu II cag/ctg 1088 Afl III a/crygt 1266 Drd I gacnnnn/nngtc 1368 AlwN I cagnnn/ctg 1677 BceF I acggc 12/13 1752 Bsa I ggtctc 1/5 2226 Fsp I tgc/gca 2379 Pvu I cgat/cg 2526 Sca I agt/act 2637 Xmn I gaann/nnttc 2754 Ssp I aat/att 2961 Aat II gacgt/c 3077
Enzyme Sites Positions: ApaL I g/tgcac 2 1580 2826 Ase I at/taat 2 1095 2330 Asp718 g/gtacc 2 304 461 BamH I g/gatcc 2 470 482 BssH II g/cgcgc 2 777 880 EcoO109 I rg/gnccy 2 765 3134 Fau I cccgc 4/6 2 798 1115 Gsu I ctggag 16/14 2 379 2244 Hinc II gty/rac 2 615 845 Kpn I ggtac/c 2 304 461 Mme I tccrac 20/18 2 1456 1640 Nsp I rcatg/y 2 903 1266 Nsp7524 I r/catgy 2 903 1266 NspC I rcatg/y 2 903 1266 Sma I ccc/ggg 2 308 465 Tfi I g/awtc 2 1101 1241 Xma I c/ccggg 2 308 465 Ban II grgcy/c 3 298 351 455 Bgl I gccnnnn/nggc 3 435 801 2273 Bsm I gaatgc 1/-1 3 532 631 876 BsmA I gtctc 1/5 3 382 2226 2991 BspH I t/catga 3 1986 2994 3099 Cfr10 I r/ccggy 3 859 899 2239 Eae I y/ggccr 3 741 1105 2547 Ear I ctcttc 1/4 3 865 1144 2948 Ecl136 I gag/ctc 3 298 351 455 Eco57 I ctgaag 16/14 3 889 1793 2841 Gdi II yggccg -5/-1 3 741 1105 2547 Sac I gagct/c 3 298 351 455 Sfe I c/tryag 3 1531 1722 2400 Tth111 II caarca 11/9 3 1840 1873 1879
Aat II 1 BssH II 2 Hga I 5 Pml I - Acc I - BstB I - HgiA I 6 PpuM I 1 Afl II - BstE II 1 Hha I 18 PshA I - Afl III 1 BstK I 15 Hinc II 2 Pst I - Age I - BstN I 6 Hind III 1 Pvu I 1 Aha II 4 BstU I 12 Hinf I 6 Pvu II 1 Alu I 16 BstX I - HinP I 18 Rma I 5 Alw I 14 BstY I 8 Hpa I 1 Rsa I 5 AlwN I 1 Bsu36 I - Hpa II 16 Rsr II - Apa I - Cfr10 I 3 Hph I 5 Sac I 3 ApaL I 2 Cla I - Kas I 1 Sac II 1 Ase I 2 Csp6 I 5 Kpn I 2 Sal I - Asp718 2 Dde I 6 Mae II 4 Sap I - Ava I 4 Dpn I 18 Mae III 10 Sau3A I 18 Ava II 5 Dpn II 18 Mbo I 18 Sau96 I 9 Avr II - Dra I 4 Mbo II 8 Sca I 1 BamH I 2 Dra III - Mcr I 4 ScrF I 15 Ban I 4 Drd I 1 Mlu I - SfaN I 7 Ban II 3 Dsa I 1 Mme I 2 Sfe I 3 Bbe I 1 Dsa V 15 Mnl I 23 Sfi I - Bbs I 1 Eae I 3 Msc I - SgrA I - Bbv I 10 Eag I - Mse I 12 Sma I 2 BceF I 1 Ear I 3 Msp I 16 Sna I - Bcl I - Ecl136 I 3 Nae I 1 SnaB I - Bcn I 9 Eco47 III - Nar I 1 Spe I - Bgl I 3 Eco57 I 3 Nci I 9 Sph I - Bgl II - EcoN I - Nco I - Spl I - Bsa I 1 EcoO109 I 2 Nde I - Sse8337 I - BsaA I - EcoR I 1 Nhe I - Ssp I 1 BsaB I 1 EcoR II 6 Nla III 11 Stu I 1 BsaJ I 8 EcoR V - Nla IV 15 Sty I - Bsg I - Ehe I 1 Not I - Swa I - BsiE I 4 Esp I - Nru I - Taq I 13 BsiY I 5 Fau I 2 Nsi I - Tfi I 2 Bsm I 3 Fse I - Nsp I 2 Tth111 I - BsmA I 3 Fnu4H I 19 Nsp7524 I 2 Tth111 II 3 Bsp120 I - Fok I 4 NspB II 5 Xba I 1 Bsp1286 I 6 Fsp I 1 NspC I 2 Xca I - BspE I - Gdi II 3 Pac I - Xcm I - BspH I 3 Gsu I 2 PaeR7 I 1 Xho I 1 BspM I - Hae I 4 PflM I - Xma I 2 BspW I 13 Hae II 4 Ple I 4 Xmn I 1 Bsr I 9 Hae III 13
1 80 CTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGT GAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAA AAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAG AGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGCTCGGTACCCGGGTCGAGTA GGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGT TTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCCCGAATTCGAGCTCGGTACCCGGGGATCCTCTAG AGGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTT TATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATG ATCCTGCAAGCCTCGTCGTCTGGCCGGACCACGCTATCTGTGCAAGGTCCCCGGACGCGCGCTCCATGAGCAGAGCGCCC GCCGCCGAGGCAAGACTCGGGCGGCGCCCTGCCCGTCCCACCAGGTCAACAGGCGGTAACCGGCCTCTTCATCGGGAATG CGCGCGACCTTCAGCATCGCCGGCATGTCCCCTGGCGGACGGGAAGTATCAGCTCGACCAAGCTTGGCGAGATTTTCAGG AGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATT TTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGCATTAATGAATCGGCCAACGCGCGGGG AGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGC GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCAC AAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC AATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGC AAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCC CGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCAC CGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGC TACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTA TCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGC CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTC AATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATA GGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAA TAGGCGTATCACGAGGCCCTTTCGTC