Plasmid name: pUHD 15-1 Size: 4455 bp Resistance: Ampicillin constructed by: Manfred Gossen Host strains: XL1 blue, DH5 alpha, E.coli "SURE" Reference: Gossen M. and Bujard H. (1992); PNAS, 89, 5547-51
The plasmid consists of three main fragments:
Afl II c/ttaag Dra III cacnnn/gtg Pst I ctgca/g
Age I a/ccggt Eag I c/ggccg Rsr II cg/gwccg
Apa I gggcc/c EcoR V gat/atc Sap I gcttcttc 1/4
Asp718 g/gtacc Esp I gc/tnagc Sfi I ggccnnnn/nggcc
Avr II c/ctagg Fse I ggccgg/cc SgrA I cr/ccggyg
Bgl II a/gatct Kpn I ggtac/c Sna I gta/tac
Bsp120 I g/ggccc Mlu I a/cgcgt Sse8337 I cctgca/gg
BspE I t/ccgga Msc I tgg/cca Stu I agg/cct
BspM I acctgc 4/8 Nhe I g/ctagc Swa I attt/aaat
BstB I tt/cgaa Not I gc/ggccgc Tth111 I gacn/nngtc
BstX I ccannnnn/ntgg Pac I ttaat/taa Xca I gta/tac
Bsu36 I cc/tnagg PflM I ccannnn/ntgg Xcm I ccannnnn/nnnntgg
Cla I at/cgat Pml I cac/gtg
Enzyme Position PaeR7 I c/tcgag 1 Xho I c/tcgag 1 Spe I a/ctagt 103 Nco I c/catgg 464 Bbs I gaagac 2/6 731 EcoR I g/aattc 766 Xba I t/ctaga 777 EcoN I cctnn/nnnagg 978 Nru I tcg/cga 1052 Nsi I atgca/t 1159 Eco47 III agc/gct 1176 Bsg I gtgcag 16/14 1315 Spl I c/gtacg 1410 Sal I g/tcgac 1538 PshA I gacnn/nngtc 1553 Sph I gcatg/c 1610 Sma I ccc/ggg 1664 Xma I c/ccggg 1664 BamH I g/gatcc 1791 Hpa I gtt/aac 1924 PpuM I rg/gwccy 2074 Bbe I ggcgc/c 2132 Ehe I ggc/gcc 2132 Kas I g/gcgcc 2132 Nar I gg/cgcc 2132 BstE II g/gtnacc 2164 Nae I gcc/ggc 2208 Hind III a/agctt 2249 Pvu II cag/ctg 2397 Drd I gacnnnn/nngtc 2677 AlwN I cagnnn/ctg 2986 Bsa I ggtctc 1/5 3535 Fsp I tgc/gca 3688 Pvu I cgat/cg 3835 Sca I agt/act 3946 Xmn I gaann/nnttc 4063 Ssp I aat/att 4270
Enzyme Sites Positions: Acc I gt/mkac 2 1430 1538 Afl III a/crygt 2 1642 2575 ApaL I g/tgcac 2 2889 4135 Ban II grgcy/c 2 668 1575 Bcl I t/gatca 2 1304 1348 BsaA I yac/gtr 2 442 1009 BsaB I gatnn/nnatc 2 1629 2023 Ecl136 I gag/ctc 2 668 1575 Gsu I ctggag 16/14 2 696 3553 Sac I gagct/c 2 668 1575 Sac II ccgc/gg 2 756 1516 SnaB I tac/gta 2 442 1009 Ase I at/taat 3 110 2404 3639 Ava I c/ycgrg 3 1 1664 2125 BspH I t/catga 3 3295 4303 4408 BssH II g/cgcgc 3 1405 2086 2189 Cfr10 I r/ccggy 3 2168 2208 3548 Dsa I c/crygg 3 464 756 1516 Eco57 I ctgaag 16/14 3 2198 3102 4150 EcoO109 I rg/gnccy 3 1441 2074 4443 Hae I wgg/ccw 3 2588 2599 3051 Nde I ca/tatg 3 337 771 1352 Sty I c/cwwgg 3 464 1310 1754 Tth111 II caarca 11/9 3 3149 3182 3188
Aat II 5 BssH II 3 Hga I 9 Pml I - Acc I 2 BstB I - HgiA I 5 PpuM I 1 Afl II - BstE II 1 Hha I 28 PshA I 1 Afl III 2 BstK I 18 Hinc II 4 Pst I - Age I - BstN I 9 Hind III 1 Pvu I 1 Aha II 10 BstU I 23 Hinf I 10 Pvu II 1 Alu I 20 BstX I - HinP I 28 Rma I 9 Alw I 12 BstY I 7 Hpa I 1 Rsa I 14 AlwN I 1 Bsu36 I - Hpa II 17 Rsr II - Apa I - Cfr10 I 3 Hph I 7 Sac I 2 ApaL I 2 Cla I - Kas I 1 Sac II 2 Ase I 3 Csp6 I 14 Kpn I - Sal I 1 Asp718 - Dde I 10 Mae II 13 Sap I - Ava I 3 Dpn I 22 Mae III 13 Sau3A I 22 Ava II 7 Dpn II 22 Mbo I 22 Sau96 I 16 Avr II - Dra I 4 Mbo II 11 Sca I 1 BamH I 1 Dra III - Mcr I 5 ScrF I 18 Ban I 4 Drd I 1 Mlu I - SfaN I 10 Ban II 2 Dsa I 3 Mme I 4 Sfe I 4 Bbe I 1 Dsa V 18 Mnl I 27 Sfi I - Bbs I 1 Eae I 4 Msc I - SgrA I - Bbv I 12 Eag I - Mse I 17 Sma I 1 BceF I 5 Ear I 4 Msp I 17 Sna I - Bcl I 2 Ecl136 I 2 Nae I 1 SnaB I 2 Bcn I 9 Eco47 III 1 Nar I 1 Spe I 1 Bgl I 6 Eco57 I 3 Nci I 9 Sph I 1 Bgl II - EcoN I 1 Nco I 1 Spl I 1 Bsa I 1 EcoO109 I 3 Nde I 3 Sse8337 I - BsaA I 2 EcoR I 1 Nhe I - Ssp I 1 BsaB I 2 EcoR II 9 Nla III 18 Stu I - BsaJ I 12 EcoR V - Nla IV 18 Sty I 3 Bsg I 1 Ehe I 1 Not I - Swa I - BsiE I 5 Esp I - Nru I 1 Taq I 13 BsiY I 10 Fau I 9 Nsi I 1 Tfi I 4 Bsm I 4 Fse I - Nsp I 5 Tth111 I - BsmA I 4 Fnu4H I 24 Nsp7524 I 5 Tth111 II 3 Bsp120 I - Fok I 4 NspB II 6 Xba I 1 Bsp1286 I 5 Fsp I 1 NspC I 5 Xca I - BspE I - Gdi II 4 Pac I - Xcm I - BspH I 3 Gsu I 2 PaeR7 I 1 Xho I 1 BspM I - Hae I 3 PflM I - Xma I 1 BspW I 24 Hae II 6 Ple I 6 Xmn I 1 Bsr I 10 Hae III 20
1 80 CTCGAGGAGCTTGGCCCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACC CTCGAGGAGCTTGGCCCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACC GCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGT TCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACG TATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGCTAAACTGCCCACTTGGC AGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCC AGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTT TGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAG TTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC GTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTT GACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCCCGAATTCATATGTCTAGATTAGATAAAAGTAAAGTG ATTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGT AGAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGC ACCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCT TTACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCA ATTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAG GTTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTA TTACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGG ATTAGAAAAACAACTTAAATGTGAAAGTGGGTCCGCGTACAGCCGCGCGCGTACGAAAAACAATTACGGGTCTACCATCG AGGGCCTGCTCGATCTCCCGGACGACGACGCCCCCGAAGAGGCGGGGCTGGCGGCTCCGCGCCTGTCCTTTCTCCCCGCG GGACACACGCGCAGACTGTCGACGGCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGCGAGGACGT GGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGACATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTA CCCCCCACGACTCCGCCCCCTACGGCGCTCTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTTACCGATGCCCTTGGA ATTGACGAGTACGGTGGGTAGGGGGCGCGAGGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTA GAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAA CCTCTACAAATGTGGTATGGCTGATTATGATCCTGCAAGCCTCGTCGTCTGGCCGGACCACGCTATCTGTGCAAGGTCCC CGGACGCGCGCTCCATGAGCAGAGCGCCCGCCGCCGAGGCAAGACTCGGGCGGCGCCCTGCCCGTCCCACCAGGTCAACA GGCGGTAACCGGCCTCTTCATCGGGAATGCGCGCGACCTTCAGCATCGCCGGCATGTCCCCTGGCGGACGGGAAGTATCA GCTCGACCAAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGA TATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGC TGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCG CTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGA TAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGA TACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTT TCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTA AGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTT CTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATT ACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACG TTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAA TCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTA TTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGC TGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCA GAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTT AATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTC CGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCG TTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCC GTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTG CCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGC GAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCT TTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAA ATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATAT TTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACC ATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC