Logo ZMBH-Abteilung Prof. Dr. Hermann Bujard
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Activation of Expression

Induction by withdrawal or by addition of Dox-HCl may take some time to be completed. This is not necessarily an intrinsic limitation of the regulatory system itself, but rather primarily a function of the half-lives of the mRNA and the protein under study: it will take some time before stably expressed proteins accumulate to equilibrium levels.

In any case, suitably regulatable clones should be subcloned. This has on occasion resulted in the identification of clones with even further improved regulatory properties.


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