University of Heidelberg

ZMBH - Central Services - Imaging Facility - Equipment


Microscope systems and current configurations (updated 04/2012)

Imaging Facility labs / sample preparation rooms

Imaging Facility course & seminar room (room -127)




Confocal microscopes:
1. Zeiss LSM 780 confocal microscope
  • Microscope stand: Zeiss Axio Observer, inverted microscope
  • Laser:
    405 nm pulsed diode laser, 30 mW
    458 nm, 488 nm, 514 nm Argon laser, 25 mW
    561 nm DPSS laser, 20 mW
    633 nm HeNe laser, 5 mW
  • (pulsed) in Tune laser 490-640 nm, 1.5 mW per wavelength
  • Objectives:
    Plan-APOCHROMAT 20x/0,80 Air
    C-APOCHROMAT 40x/1.2 W Korr-UV-VIS-IR
    Plan-APOCHROMAT 40x/1.3 Oil
    Plan-APOCHROMAT 63x/1.4 Oil
    Plan-APOCHROMAT 100x/1.4 Oil
  • Software: Zeiss ZEN 2010
  • Room -132
2. Leica TCS SP5 confocal microscope
  • Microscope stand: Leica DMI 6000 CS Trino, inverted microscope
  • Laser:
    405 nm diode laser, 50 mW
    458 nm, 5 mW, 476 nm, 5mW, 488 nm, 20 mW, 514 nm, 20 mW Argon laser
    561 nm DPSS laser, 20 mW
    633 nm HeNe laser, 10 mW
  • Objectives:
    HC PL APO 10x/0.40 CS Air
    HCX PL APO 20x/0.70 IMM CORR Lbd BL
    HCX Plan APO 40x/1.30 Oil CS
    HCX PL APO 63x/1.20 W CORR CS
    HCX PL APO 63x/1.30 GLYC CORR CS
    HCX PL APO 63x/1.40-0.60 Oil Lbd BL
  • 3 Hybrid GaAsp detectors (HyD) and 2 PMTs
  • Tandem scanning system
  • Incubation chamber w/ Temp & CO2 control (LIS)
  • Software: Leica Application Suite (LAS)
  • Room -132

3. Leica TCS SP2 confocal microscope
  • Microscope stand: Leica DMIRE2, inverted microscope
  • Laser:
    405 nm pulsed diode laser, 1 mW
    458 nm, 476 nm, 488 nm, 514 nm Argon laser
    561 nm DPSS laser, 10 mW
    633 nm HeNe laser, 10 mW
  • Objectives:
    HC PL FLUOTAR 20x/0.50 Air
    HC PL APO 20x/0.70 IMM CORR CS
    HCX Plan APO 40x/0.85 CORR Air
    HCX PL APO 63x/1.20 W CORR Lambda BL
    HCX PL APO 63x/1.40-0.60 Oil Lambda BL
    PL APO 100x/1.40-0.7 Oil
  • Software: Leica Confocal Software (LCS)
  • Room -144
4. PerkinElmer UltraVIEW ERS Spinning Disk microscope
  • Microscope stand: Zeiss Axiovert 200M, inverted microscope
  • Confocal Scanner Unit: Yokogawa CSU 22
  • Laser:
    405 nm, 440 nm, 640 nm diode laser
    488 nm, 514 nm Argon laser
    568 nm Krypton laser
  • Objectives:
    EC Plan-NEOFLUAR 20x/0.5 Air
    EC Plan-NEOFLUAR 40x/1.3 Oil
    Plan-APOCHROMAT 63x/1.4 Oil DIC
    Plan-APOCHROMAT 100x/1.4 Oil DIC
  • Camera: Hamamatsu EMCCD (C9100-50)
  • Software: UltraVIEW ERS Imaging Suite
  • Incubation chamber XL & Pecon temperature control unit
  • Room -132

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Superresolution/TIRF/ScannerFRAP widefield microscope:
5. Olympus xcellence 3D PALM/STORM/TIRF/ScannerFRAP
  • Microscope stand: Olympus IX81, inverted microscope
  • Olympus cellTIRF 4-Line Motorized TIRF Combiner
  • TIRF Lasers:
    405 nm, 100 mW
    488 nm, 100 mW
    561 nm, 100 mW
    640 nm, 100 mW
  • Olympus cellFRAP module
  • ScannerFRAP Laser:
    514 nm, 100 mW
  • Objectives:
    UPLSAPO 60x/1.35 Oil DIC
    PLAPO 100x/1.45 Oil DIC
  • MT 20 illumination system:
    150 W Xe or 150 W Hg/Xe arc burner
    Fast filter wheel
  • Filters:
    Dualband CFP/YFP sbx HC filter set
    Dualband GFP/mCherry sbx ET filter set
    Quad DAPI/FITC/Cy3(Texas Red)/Cy5 sbx HC filter set
    TIRF Quad
  • Cameras:
    Olympus XM10
    Hamamatsu ImagEM Enhanced (C9100-13)
  • Software: Olympus xcellence & QuickPALM
  • Custom-made astigmatic lens for 3D PALM/STORM
  • Incubation chamber & Pecon temperatur control unit
  • TMC Linos optical table 63-550
  • Room -132

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Widefield microscopes:
6. Olympus xcellence PointFRAP microscope
  • Microscope stand: Olympus IX81, inverted microscope
  • Laser:
    488 nm, 100 mW
  • Objectives:
    UPLSAPO 10x/0.4 Air
    UPLSAPO 20x/0.75 Air
    UPLSAPO 40x/0.95 Air DIC
    UPLSAPO 60x/1.35 Oil
    PLAPO 100x/1.45 Oil DIC
  • MT 20 illumination system:
    150 W Xe or 150 W Hg/Xe arc burner
    Fast filter wheel (minimal switch 58ms)
  • Filters:
    Dualband CFP/YFP sbx HC filter set
    Dualband GFP/mCherry sbx ET filter set
    Quad DAPI/FITC/Cy3(Texas Red)/Cy5 sbx HC filter set
  • Camera: Hamamatsu ORCA-R2
  • Software: Olympus xcellence
  • Okolab microscope stage incubation system, Lauda low-temp thermostat
  • Room -144
7. Leica DMIRE2, inverted microscope
  • Objectives:
    N PLAN 10x/0.22 PH1 Air
    N PLAN L 20x/0.40 CORR Ph1 Air
    N PLAN L 40x/0.55 CORR PH2 Air
    PL APO 40x/1.25-0.75 Oil
    HCX PL APO 63x/1.40-0.60 Oil
    HCX PL APO 100x/1.40-0.70 Oil CS
  • Filters:
    Ex 470/40, Em 525/50
    Ex BP 436/20, Em BP 480/40
    Ex BP 510/20, Em BP 560/40
    Ex BP 510/40, Em LP 610
  • Camera: Hamamatsu ORCA-ER (C4742-80-12AG)
  • Software: OpenLab 5.0.2
  • Room -144

8. Leica DMRXA, upright microscope
  • Objectives:
    N Plan 5x/0.12 PH0 Air
    HC PL Fluotar 10x/0.30 PH1 Air
    HC PL Fluotar 20x/0.50 PH2 Air
    PL Fluotar 40x/0.70 PH2 Air
    PL APO 40x/1.25-0.75 Oil
    PL APO 63x/1.0 W CORR
    PL APO 100x/1.40-0.7 Oil
  • Filters:
    Ex 340-380, Em LP 425 (DAPI)
    Ex BP 450-490, Em LP 515 (FITC)
    Ex 470/40, Em BP 525/50 (GFP)
    Ex 470/40, Em LP 500 (EGFP)
    Ex BP 620/60, Em LP 700/75 (Cy5)
    Ex 531-560, Em BP 574-648 (TexasRed)
    Ex BP 436/20, Em BP 480/40 (CFP)
    Ex BP 560/40, Em BP 645/75 (Cy3)
  • Camera: Hamamatsu ORCA-ER (C4742-95-12ER)
  • Software: OpenLab 4.0.2
  • Room -144
9. Leica DMIRB, inverted microscope
  • Objectives:
    N Plan 5x/0.11 PH0 Air
    N Plan 10x/0.22 PH1 Air
    HC PL APO 20x/0.70 IMM CORR CS
    N PLAN L 40x/0.55 CORR Ph2 Air
    PL FLUOTAR L 63x/0.70 CORR Ph2 Air
  • Filters:
    Ex 565/30, Em 620/60
    Ex BP 340-380, Em LP 425
    Ex BP 450-490, Em LP 515
    Ex 500/20, Em 535/30
  • Camera: Hamamatsu ORCA-100 (C4742-95)
  • Software: OpenLab 4.0.2
  • Room -144

10. Leica DMRXE, upright microscope
  • Objectives:
    PL Fluotar 2.5x/0.07 Air
    PL Fluotar 10x/0.30 Air PH1
    PL Fluotar 20x/0.50 Air PH2
    PL Fluotar 40x/0.70 Air PH2
    PL Fluotar 100x/1.30 Oil PH3
  • Filters:
    Ex BP 340-380, Em LP 425
    Ex BP 450-490, Em BP 515-560
    Ex 530-595, Em LP 615
    Ex 570/50, Em 640/40
  • Camera: Jenoptik ProgRes C10plus
  • Software: ProgRes CapturePro
  • Room -127
11. Zeiss Axiophot, upright microscope
  • Objectives:
    Plan NEOFLUAR 10x/0.30 Air
    Plan NEOFLUAR 20x/0.50 Air
    Plan NEOFLUAR 40x/0.75 Air
    Plan-APOCHROMAT 63x/1.40 Oil
    Plan NEOFLUAR 100x/1.30 Oil Iris
  • Filters:
    Ex G 365, Em LP 420
    Ex BP 546, Em LP 590
    Ex 450-490, Em LP 520
  • Camera: Jenoptik ProgRes MF USB
  • Software: ProgRes CapturePro
  • Room -127

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Micromanipulation/-injection system:
12. AIS 2 micromanipulation system
  • Microscope stand: Zeiss Axiovert 10, inverted microscope
  • Injection system: Luigs & Neumann, Germany
  • Injection pressure system: Eppendorf FemtoJet
  • Objectives:
    ACHROSTIGMAT 5x/0.12 Air
    ACHROSTIGMAT 10x/0.25 Air
    ACHROSTIGMAT 20x/0.30 Air Ph1
    ACHROSTIGMAT 32x/0.40 Air Ph1
    Plan NEOFLUAR 40x/0.75 Air
  • Filters:
    F41-017 HQ-Set EGFP
    F41-021 HQ-Set Cy 3,5
  • Camera: ImagingSource DMK2002
  • Software: AIS 2 control program
  • Room -144

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Stereomicroscopes & macroscopes:
13. Leica MZ FLIII fluorescence stereomicroscope
  • Filters: GFP2, CFP, UV, G(reen) ex.
  • Cold light source: Leica KL 1500 LCD
  • Fluorescence light source: Leica HXP 120W
  • Camera: Olympus DP50-CU (optional)
  • Software: Viewfinder Lite version 1.0
  • Room -144
14. Leica M420 macroscope
  • Cold light sources: Leica KL 1500 LCD & Zeiss KL 2500 LCD
  • Camera: Olympus DP50-CU (optional)
  • Software: Viewfinder Lite version 1.0
  • Room -144

15. Leica Wild M3Z stereomicroscope
  • Room -144

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Imaging Facility labs / sample preparation rooms:

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