Ruprecht-Karls-Universität Heidelberg

Contact us:
Zentrum fr Molekulare Biologie der Universitt Heidelberg (ZMBH)
Im Neuenheimer Feld 282
Room 401
69120 Heidelberg, Germany
Tel.: +49-6221-54 6813
Fax: +49-6221-54 5894
ms-service@zmbh.uni-heidelberg.de

Protein identification(s)

We reliably and sensitively identify proteins from low-complex (e.g. single gel spots, purified protein etc.) as well as complex samples by our standard identification workflow (Figure 1). In this, proteins are first proteolitically digested to peptides (gel-based or gel-free) and then analyzed by LC-MS/MS using our highly sensitive Orbitrap-MS systems. Afterwards, peptides/proteins are identified by a MASCOT database search (MS/MS Ion Search) and results are imported into and presented with the Scaffold software package. To account for more complex samples, different pre-fractionation methods and longer LC-gradients are also available. In addition, on request the data can also be searched with additional search engines like Sequest or Andromeda (MaxQuant).


Workflow
                                      Protein Identification
Figure 1 - Protein identification workflow utilized in the Core Facility