Our research group has moved to the University of Bonn!
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Nuclear processes during the cell cycle
Research in our group focuses on the analysis of nuclear processes during different stages of the cell cycle. We are using both a well-estalished cell free system based on extracts of amphibian eggs and living mammalian cells to analyse the regulation of spindle function in M-phase as well as aspects of the regulation of nuclear integrity in interphase.
The mitotic spindle is a large macromolecular complex consisting of several hundreds of proteins, which contribute to microtubule nucleation and organisation, interaction of microtubules with the chromosomes and regulation of cell cycle progression. In higher eukaryotes, the spindle can only form after breakdown of the nucleus at mitotic onset and activation of nuclear proteins for spindle assembly. The small GTPase Ran plays an essential role in this regulation. We are analysing the function and the regulation of nuclear microtubule-binding proteins using approaches in living cells and in cell free extracts.
In the nucleus of metabolically active cells, proteins sharing common functions are selectively concentrated in subnuclear domains or nuclear bodies. A paradigm therefore is the Cajal body accumulation of the SMN complex, a central component for the maturation of splicing factors, which is also present in the cytoplasm. By the use of biochemical experiments and targeting studies in living mammalian cells, we are analysing modification patterns and regulators of the SMN complex, which define its specific subcellular localisation and function.
Selected publications
- Kulturmus, B., Wang, W., Ruppert, T., Neuner, A., Cerikan, B., Viol, L., Duenas-Sanchez, R., Gruss, O.J. and Pereira, G. WDR8 is a centriolar satellite and centriole-associate protein that promotes ciliary vesicle docking during ciliogenesis, J Cell Sci, accepted for publication (2015)
- Chinen, T., Liu, P., Shioda, S., Pagel, J., Cerikan, B., Lin, T. C., Gruss, O.J., Hayashi, Y., Takeno, H., Shima, T., Okada, Y., Hayakawa, I., Kigoshi, H., Usui, T., Schiebel, E, 2015 The gamma-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun. 6, 8722-30
- Husedzinovic A, Neumann B, Reymann J, Draeger-Meurer S, Chari A, Erfle H, Fischer U, Gruss OJ. The catalytically inactive tyrosine phosphatase HD-PTP/PTPN23 is a novel regulator of SMN complex localization. Mol Biol Cell. 2014 Nov 12. pii: mbc.E14-06-1151.
- Tegha-Dunghu J, Bausch E, Neumann B, Wuensche A, Walter T, Ellenberg J, Gruss OJ. MAP1S controls microtubule stability throughout the cell cycle in human cells J Cell Sci.2014 Oct 9.pii: jcs. 136457.
- Klinger M, Wang W, Kuhns S, Baerenz F, Dräger-Meurer S, Pereira G, Gruss OJ. The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone. Mol Biol Cell. 25(4):495-507, Feb (2014)
- Yokoyama H, Koch B, Walczak R, Ciray-Duygu F, González-Sánchez JC, Devos DP, Mattaj IW, Gruss OJ. The nucleoporin MEL-28 promotes RanGTP-dependent g-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun. 5:3270, Feb 10 (2014)
- Baerenz F, Inoue D, Yokoyama H, Tegha-Dunghu J, Freiss S, Draeger S, Mayilo D, Cado I, Merker S,Hoeckendorf B, Pilz S, Steinbeisser H, Lorenz H, Ruppert T, Wittbrodt J and Gruss OJ. The centriolar satellite protein SSX2IP promotes centrosome maturation. J Cell Biol, July 2013
- Baerenz F, Mayilo D, Gruss OJ. Centriolar Satellites: busy orbits around the centrosome, Eur J Cell Biol. 2011 Dec; 90(12): 983-9. doi: 10.1016/j.ejcb.2011.07.007. Epub 2011 Sep 25.Review
- Gruss OJ, Nuclear transport receptor goes moonlighting, Nat Cell Biol, 12: 640-41 (2010)
- Reber S, Over S, Kronja I and Gruss OJ. CaM kinase II Initiates meiotic spindle depolymerisation independently of APC/C activation, J Cell Biol, 183: 1007-17 (2008)
- Tegha-Dunghu J, Neumann B, Reber S, Krause R Erfle H, Walter T, Held M, Rogers P, Hupfeld K, Ruppert T, Ellenberg, J and Gruss OJ. Eml3 is a nuclear microtubule binding protein required for proper alignment of chromosomes in metaphase. J Cell Sci, 121: 1718-26 (2008)
- Petri S, Grimmler M, Over S, Fischer U, Gruss OJ. Dephosphorylation of SMN by PPM1G/PP2Cγ governs Cajal body localization and stability of the SMN complex. J Cell Biol, 179(3): 451-65 (2007)
- Gruss OJ and Vernos I, The mechanism of spindle assembly. Functions of Ran and its target TPX2, J Cell Biol, 166: 949-955 (2004)
- Gruss OJ, Wittmann M, Yokoyama H, Pepperkok R, Kufer TA, Silljé HHW, Karsenti E, Mattaj IW, Vernos I. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells, Nat Cell Biol, 4: 871-9 (2002)
- Gruss OJ, Carazo-Salas RE, Schatz CA, Guarguaglini G, Kast J, Wilm M, Le Bot N, Vernos I, Karsenti E, Mattaj IW. Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity. Cell, 104:
83-93 (2001)
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Research in our group focuses on the analysis of nuclear processes during different stages of the cell cycle. We are using both a well-estalished cell free system based on extracts of amphibian eggs and living mammalian cells to analyse the regulation of spindle function in M-phase as well as aspects of the regulation of nuclear integrity in interphase.
Selected publications - Kulturmus, B., Wang, W., Ruppert, T., Neuner, A., Cerikan, B., Viol, L., Duenas-Sanchez, R., Gruss, O.J. and Pereira, G. WDR8 is a centriolar satellite and centriole-associate protein that promotes ciliary vesicle docking during ciliogenesis, J Cell Sci, accepted for publication (2015) - Chinen, T., Liu, P., Shioda, S., Pagel, J., Cerikan, B., Lin, T. C., Gruss, O.J., Hayashi, Y., Takeno, H., Shima, T., Okada, Y., Hayakawa, I., Kigoshi, H., Usui, T., Schiebel, E, 2015 The gamma-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun. 6, 8722-30
- Tegha-Dunghu J, Bausch E, Neumann B, Wuensche A, Walter T, Ellenberg J, Gruss OJ. MAP1S controls microtubule stability throughout the cell cycle in human cells J Cell Sci.2014 Oct 9.pii: jcs. 136457. - Klinger M, Wang W, Kuhns S, Baerenz F, Dräger-Meurer S, Pereira G, Gruss OJ. The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone. Mol Biol Cell. 25(4):495-507, Feb (2014) - Yokoyama H, Koch B, Walczak R, Ciray-Duygu F, González-Sánchez JC, Devos DP, Mattaj IW, Gruss OJ. The nucleoporin MEL-28 promotes RanGTP-dependent g-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun. 5:3270, Feb 10 (2014) - Baerenz F, Inoue D, Yokoyama H, Tegha-Dunghu J, Freiss S, Draeger S, Mayilo D, Cado I, Merker S,Hoeckendorf B, Pilz S, Steinbeisser H, Lorenz H, Ruppert T, Wittbrodt J and Gruss OJ. The centriolar satellite protein SSX2IP promotes centrosome maturation. J Cell Biol, July 2013 - Baerenz F, Mayilo D, Gruss OJ. Centriolar Satellites: busy orbits around the centrosome, Eur J Cell Biol. 2011 Dec; 90(12): 983-9. doi: 10.1016/j.ejcb.2011.07.007. Epub 2011 Sep 25.Review - Gruss OJ, Nuclear transport receptor goes moonlighting, Nat Cell Biol, 12: 640-41 (2010) - Reber S, Over S, Kronja I and Gruss OJ. CaM kinase II Initiates meiotic spindle depolymerisation independently of APC/C activation, J Cell Biol, 183: 1007-17 (2008) - Tegha-Dunghu J, Neumann B, Reber S, Krause R Erfle H, Walter T, Held M, Rogers P, Hupfeld K, Ruppert T, Ellenberg, J and Gruss OJ. Eml3 is a nuclear microtubule binding protein required for proper alignment of chromosomes in metaphase. J Cell Sci, 121: 1718-26 (2008) - Petri S, Grimmler M, Over S, Fischer U, Gruss OJ. Dephosphorylation of SMN by PPM1G/PP2Cγ governs Cajal body localization and stability of the SMN complex. J Cell Biol, 179(3): 451-65 (2007) - Gruss OJ and Vernos I, The mechanism of spindle assembly. Functions of Ran and its target TPX2, J Cell Biol, 166: 949-955 (2004) - Gruss OJ, Wittmann M, Yokoyama H, Pepperkok R, Kufer TA, Silljé HHW, Karsenti E, Mattaj IW, Vernos I. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells, Nat Cell Biol, 4: 871-9 (2002) - Gruss OJ, Carazo-Salas RE, Schatz CA, Guarguaglini G, Kast J, Wilm M, Le Bot N, Vernos I, Karsenti E, Mattaj IW. Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity. Cell, 104: |
