TBLASTN 1.3.9 [29-Oct-93]

Reference:  Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers,
and David J. Lipman (1990).  Basic local alignment search tool.  J. Mol. Biol.
215:403-410.

Notice:  statistical significance is estimated under the assumption that the
equivalent of one complete reading frame of the database codes for protein and
that significant alignments will involve only coding reading frames.

Query=  D09_orf127a.prot 127 bases, CA4A80B8 checksum.
        (127 letters)

Database:  MGseq
           1 sequences; 580,073 total letters.
Searchingdone

                                                                     Smallest
                                                                     Poisson
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N

Mycoplasma genitalium 580073 bp 10/09/95                  +2   383  3.9e-50   1



>Mycoplasma genitalium 580073 bp 10/09/95
            Length = 580,073

  Plus Strand HSPs:

 Score = 51 (23.9 bits), Expect = 0.27, P = 0.23
 Identities = 8/28 (28%), Positives = 21/28 (75%), Frame = +2

Query:     59 LIVLGVLEILLMVAQLVMSNMAANIINE 86
              + +L +  ++LMV +L++S + +N++N+
Sbjct: 111632 IAILFITRLMLMVRKLMLSLILSNLVNK 111715

 Score = 46 (21.6 bits), Expect = 0.42, Poisson P(2) = 0.34
 Identities = 14/62 (22%), Positives = 25/62 (40%), Frame = +1

Query:     14 TMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQ 73
              T + C F F     ++     YDL     +  P  + +F   N   I+  V  + +M+A 
Sbjct: 107905 TRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPF*EKLFNEQNYQKIIDSVSRLNVMIAN 108084

Query:     74 LV 75
               +
Sbjct: 108085 YI 108090

 Score = 44 (20.7 bits), Expect = 0.42, Poisson P(3) = 0.35
 Identities = 7/19 (36%), Positives = 12/19 (63%), Frame = +3

Query:     2 KVIKKLNLLNELTMLVCIF 20
             K    L  +NELT ++C++
Sbjct: 80370 KASNSLRCINELTSILCLY 80426

 Score = 43 (20.2 bits), Expect = 0.27, Poisson P(4) = 0.24
 Identities = 9/16 (56%), Positives = 10/16 (62%), Frame = +2

Query:     91 VEQKFAKALKWSRFLP 106
              VEQ+  K   W RFLP
Sbjct: 306755 VEQQSPKLSVWVRFLP 306802

 Score = 42 (19.7 bits), Expect = 1.9, P = 0.85
 Identities = 8/17 (47%), Positives = 12/17 (70%), Frame = +2

Query:   105 LPFGLLQLYCYHKIKLV 121
             +PF ++ L CYH + LV
Sbjct: 71429 VPF*VVFLTCYHSLFLV 71479

 Score = 42 (19.7 bits), Expect = 2.5, Poisson P(2) = 0.92
 Identities = 9/20 (45%), Positives = 11/20 (55%), Frame = +2

Query:      8 NLLNELTMLVCIFFFVCTIS 27
              NL+ +L    CIFF    IS
Sbjct: 416078 NLITKLANFSCIFFTTMDIS 416137

 Score = 41 (19.3 bits), Expect = 3.2, Poisson P(2) = 0.96
 Identities = 8/20 (40%), Positives = 13/20 (65%), Frame = +2

Query:      7 LNLLNELTMLVCIFFFVCTI 26
              +N+++    L+CIFF   TI
Sbjct: 524942 INVVSTNYFLICIFFNTFTI 525001

 Score = 40 (18.8 bits), Expect = 4.7, Poisson P(4) = 0.99
 Identities = 9/20 (45%), Positives = 11/20 (55%), Frame = +3

Query:    107 FGLLQLYCYHKIKLVTQTDN 126
              FGLL L+   K K + Q  N
Sbjct: 156258 FGLLYLWIVKKSKQIAQQPN 156317

  Minus Strand HSPs:

 Score = 383 (179.9 bits), Expect = 3.9e-50, P = 3.9e-50
 Identities = 80/127 (62%), Positives = 92/127 (72%), Frame = -3

Query:     1 MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLI 60
             MKV KKL+ LNE+ +L+ IFF VC ISL+GIGI++DLI + SLSA R DPIF NL  VLI
Sbjct: 64911 MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLI 64732

Query:    61 VLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALKWSRFLPFGLLQLYCYHKIKL 120
             VLGV  I  M+ QLV+S M    IN   EN+E KF K L  S FLPFGLLQLYCY KIKL
Sbjct: 64731 VLGVFAICFMIIQLVISIMI*KTINTACENIEPKFKKILH*SCFLPFGLLQLYCYQKIKL 64552

Query:   121 VTQTDNI 127
             V Q DN+
Sbjct: 64551 VKQADNL 64531


Parameters:
  E = 5.0, S = 43 (20.2 bits),  E2 = 0.12, S2 = 35
  W = 3, T = 13 (6.1 bits), X = 22 (10.3 bits)
  M = BLOSUM62
  C = 0 (Standard genetic code)
  H = 0, V = 500, B = 250
  -gapdecayrate 0.5 (the default)

Statistics:
  Lambda = 0.326, Lambda/ln2 = 0.470, K = 0.142, H = 0.567 bits/position
  Expected/Observed high score = 45 (21.1 bits) / 383 (179.9 bits)
  # of letters in query:  127 aa
  # of neighborhood words in query:  761
  # of exact words scoring below T:  8
  Database:  MGseq
  # of letters in database:  580,073 bp
  # of word hits against database:  85,509
  # of failed hit extensions:  74,022
  # of successful extensions:  353
  # of overlapping HSPs discarded:  344
  # of HSPs reportable:  9
  # of sequences in database:  1
  # of db sequences with at least one HSP:  1
No. of states in DFA:  339 (34 KB)
Total size of DFA:  43 KB (64 KB)
Time to generate neighborhood:  0.00u 0.01s 0.01t  Real: 00:00:00
No. of processors used:  1
Time to search database:  1.65u 0.03s 1.68t  Real: 00:00:03
Total cpu time:  1.68u 0.18s 1.86t  Real: 00:00:03