TBLASTN 1.3.9 [29-Oct-93]
Reference: Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers,
and David J. Lipman (1990). Basic local alignment search tool. J. Mol. Biol.
215:403-410.
Notice: statistical significance is estimated under the assumption that the
equivalent of one complete reading frame of the database codes for protein and
that significant alignments will involve only coding reading frames.
Query= P01_orf341.prot 341 bases, 4174C66E checksum.
(341 letters)
Database: MGseq
1 sequences; 580,073 total letters.
Searchingdone
Smallest
Poisson
Reading High Probability
Sequences producing High-scoring Segment Pairs: Frame Score P(N) N
Mycoplasma genitalium 580073 bp 10/09/95 +3 1011 3.6e-138 1
>Mycoplasma genitalium 580073 bp 10/09/95
Length = 580,073
Plus Strand HSPs:
Score = 1011 (472.3 bits), Expect = 3.6e-138, P = 3.6e-138
Identities = 186/338 (55%), Positives = 259/338 (76%), Frame = +3
Query: 1 MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQL 60
M+KL+SILVPCY+S+PFL RFF SLLKQD N K+IFFNDN D TYE+L++F++ H L
Sbjct: 420333 MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNL 420512
Query: 61 AIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAV 120
AIEV+C ++N G+G RD+L+N V TPYFYF+DPDD F++ N K IVE+I+ E+FD+ V
Sbjct: 420513 AIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGV 420692
Query: 121 LNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFF 180
L S+VYL LK+DF+IK +PLK IFQG+VKL +N VN L+YI+NNDQYI NIVINT FF
Sbjct: 420693 LKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYI*NIVINTDFF 420872
Query: 181 KALDLQFVNRFIEDIAVWFPIMFKAQKVLWIDVNGVNYYLRPNSASTQKNSIKLLSFIEA 240
+ L+L F +R EDI + +P+ F +QK+++IDV G NY++R +S ST ++ + L+ I+
Sbjct: 420873 RKLNLTFESRLFEDIPI*YPMFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQC 421052
Query: 241 YERLYFHLKKVGKLADFIDPNNKIESRFWRRQAFIWFSFINVSWMKAEFEQTKSVLQKLF 300
YE+LY +L + G LA FIDPN+KIE+RF RRQ F+ F+ + + K F ++K +L+KLF
Sbjct: 421053 YEKLYVNLSQNGSLASFIDPNHKIEARF*RRQMFV*FALFSFEYFKKNFSESKKILEKLF 421232
Query: 301 DFMEANGIYDRVFTNKHHGIYLLWVNRLKHFKKLVQAQ 338
F+E NG+Y+RVF K+ GIY +WV RLK+FK +++++
Sbjct: 421233 VFLEKNGVYERVFQTKNQGIYYIWVQRLKYFKHVLESK 421346
Score = 39 (18.2 bits), Expect = 2.0, P = 0.86
Identities = 6/10 (60%), Positives = 8/10 (80%), Frame = +2
Query: 330 HFKKLVQAQP 339
HF KL++ QP
Sbjct: 206510 HFNKLIELQP 206539
Minus Strand HSPs:
Score = 49 (22.9 bits), Expect = 0.72, Poisson P(2) = 0.51
Identities = 8/16 (50%), Positives = 12/16 (75%), Frame = -3
Query: 196 AVWFPIMFKAQKVLWI 211
+VW P++FK KVL +
Sbjct: 204384 SVWIPLLFKQLKVLTV 204337
Score = 49 (22.9 bits), Expect = 0.72, Poisson P(2) = 0.51
Identities = 9/23 (39%), Positives = 16/23 (69%), Frame = -3
Query: 122 NSIVYLQMLKNDFLIKHIPLKNI 144
N ++L+MLK +F + +P+ NI
Sbjct: 411012 NK*IHLKMLKKNFNLLQLPVMNI 410944
Score = 47 (22.0 bits), Expect = 0.89, Poisson P(3) = 0.59
Identities = 10/20 (50%), Positives = 12/20 (60%), Frame = -3
Query: 20 RFFKSLLKQDWNGVKVIFFN 39
R F+S+ K WN K FFN
Sbjct: 481815 REFQSVSKNPWNRSKKRFFN 481756
Score = 47 (22.0 bits), Expect = 1.8, Poisson P(2) = 0.83
Identities = 8/24 (33%), Positives = 14/24 (58%), Frame = -3
Query: 165 NNDQYIWNIVINTAFFKALDLQFV 188
NND+ ++N N + LD +F+
Sbjct: 556608 NNDKNLFNATFNCFLYYVLDSRFI 556537
Score = 46 (21.5 bits), Expect = 1.8, Poisson P(3) = 0.83
Identities = 9/23 (39%), Positives = 15/23 (65%), Frame = -3
Query: 211 IDVNGVNYYLRPNSASTQKNSIK 233
I ++G +YYL NS+S + I+
Sbjct: 380013 IGIDGGSYYLTKNSSSKTERDIE 379945
Score = 45 (21.0 bits), Expect = 1.9, Poisson P(4) = 0.85
Identities = 8/32 (25%), Positives = 19/32 (59%), Frame = -1
Query: 276 WFSFINVSWMKAEFEQTKSVLQKLFDFMEANG 307
WF + ++K + +++S + + + +EANG
Sbjct: 246107 WFFW*GAHFLKGKSFKSRSWISRNLNLLEANG 246012
Score = 45 (21.0 bits), Expect = 3.2, Poisson P(3) = 0.96
Identities = 13/40 (32%), Positives = 17/40 (42%), Frame = -1
Query: 78 DQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFD 117
DQL+ V+ + DE DP F AI+ T D
Sbjct: 301304 DQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVD 301185
Score = 44 (20.6 bits), Expect = 5.0, Poisson P(3) = 0.99
Identities = 9/25 (36%), Positives = 15/25 (60%), Frame = -2
Query: 237 FIEAYERLYFHLKKVGKLADFIDPN 261
FI+ + +L F+ KK+ + F D N
Sbjct: 455839 FIDGFFKLEFNQKKIEEKVQFNDHN 455765
Score = 43 (20.1 bits), Expect = 6.6, Poisson P(3) = 1.0
Identities = 8/21 (38%), Positives = 13/21 (61%), Frame = -2
Query: 149 VKLNPDNTVNHLHYIQNNDQY 169
VK+ N+VN + ++NN Y
Sbjct: 517675 VKVKIGNSVNEITLLRNNKNY 517613
Score = 43 (20.1 bits), Expect = 6.6, Poisson P(3) = 1.0
Identities = 10/31 (32%), Positives = 16/31 (51%), Frame = -1
Query: 138 HIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQ 168
H N+F +K D T+ + + Q+NDQ
Sbjct: 132461 HKQQHNVFVLDLKNRADLTIAQVAWAQHNDQ 132369
Score = 42 (19.6 bits), Expect = 7.6, Poisson P(3) = 1.0
Identities = 7/20 (35%), Positives = 13/20 (65%), Frame = -2
Query: 260 PNNKIESRFWRRQAFIWFSF 279
PN IE +F+ ++ +F+F
Sbjct: 492784 PNQLIERQFYLKEVLSFFNF 492725
Score = 42 (19.6 bits), Expect = 7.6, Poisson P(3) = 1.0
Identities = 10/26 (38%), Positives = 13/26 (50%), Frame = -3
Query: 36 IFFNDNKPDPTYEILKQFQQAHPQLA 61
+F N +KPD + KQ Q LA
Sbjct: 257073 VFLNHHKPDSG*LLFKQVQSVQLWLA 256996
Score = 42 (19.6 bits), Expect = 7.6, Poisson P(3) = 1.0
Identities = 9/34 (26%), Positives = 21/34 (61%), Frame = -3
Query: 173 IVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQ 206
+++ TAF L++ ++++ ++ FPI FK +
Sbjct: 199587 LLVCTAFKAFLEIFCTSKWMGVVSFVFPINFKGR 199486
Score = 41 (19.2 bits), Expect = 1.1e+02, Poisson P(7) = 1.0
Identities = 5/11 (45%), Positives = 8/11 (72%), Frame = -1
Query: 272 QAFIWFSFINV 282
+ F WF F+N+
Sbjct: 269195 EVFFWFRFLNI 269163
Score = 41 (19.2 bits), Expect = 1.1e+02, Poisson P(7) = 1.0
Identities = 8/19 (42%), Positives = 11/19 (57%), Frame = -3
Query: 134 FLIKHIPLKNIFQGKVKLN 152
FLI N++Q +KLN
Sbjct: 395280 FLINETTKNNLYQNALKLN 395224
Score = 41 (19.2 bits), Expect = 15., Poisson P(4) = 1.0
Identities = 8/23 (34%), Positives = 13/23 (56%), Frame = -2
Query: 161 HYIQNNDQYIWNIVINTAFFKAL 183
H++ N+ Y+ I+ A F AL
Sbjct: 179185 HFLSRNESYLHLIIFLHAGFDAL 179117
Score = 41 (19.2 bits), Expect = 31., Poisson P(5) = 1.0
Identities = 10/25 (40%), Positives = 12/25 (48%), Frame = -1
Query: 244 LYFHLKKVGKLADFIDPNNKIESRF 268
L + K G F DPNNK+ F
Sbjct: 527102 LISYQNKQGIALIFNDPNNKLVKNF 527028
Score = 41 (19.2 bits), Expect = 1.1e+02, Poisson P(7) = 1.0
Identities = 8/26 (30%), Positives = 17/26 (65%), Frame = -2
Query: 242 ERLYFHLKKVGKLADFIDPNNKIESR 267
++ + H +VGKLA + NK++++
Sbjct: 286246 DKRFQHCLRVGKLAKQLAIANKLDAK 286169
Score = 41 (19.2 bits), Expect = 59., Poisson P(6) = 1.0
Identities = 9/26 (34%), Positives = 13/26 (50%), Frame = -1
Query: 253 KLADFIDPNNKIESRFWRRQAFIWFS 278
KL F P NK+ S ++ WF+
Sbjct: 208307 KLRKFFLPKNKVASALSFSRSNCWFN 208230
Score = 41 (19.2 bits), Expect = 31., Poisson P(5) = 1.0
Identities = 7/33 (21%), Positives = 19/33 (57%), Frame = -2
Query: 104 FKAIVETIQGENFDIAVLNSIVYLQMLKNDFLI 136
F+ + + + F + + +++V QML + F++
Sbjct: 275938 FQTLHKALLNSPFQVQLTDTLVQKQMLSSPFVL 275840
Score = 40 (18.7 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 5/13 (38%), Positives = 11/13 (84%), Frame = -2
Query: 5 ISILVPCYQSQPF 17
++++VPC+Q+ F
Sbjct: 322390 LTLMVPCFQATKF 322352
Score = 40 (18.7 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 8/18 (44%), Positives = 10/18 (55%), Frame = -2
Query: 54 QQAHPQLAIEVHCGEKNV 71
Q+AH L + HC K V
Sbjct: 345715 QKAHEFLVSKTHCFSKKV 345662
Score = 40 (18.7 bits), Expect = 4.0, Poisson P(2) = 0.98
Identities = 8/21 (38%), Positives = 13/21 (61%), Frame = -2
Query: 8 LVPCYQSQPFLDRFFKSLLKQ 28
++P YQS F + K L+K+
Sbjct: 72217 VIPVYQSSSFEWKLPKQLIKE 72155
Score = 40 (18.7 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 11/23 (47%), Positives = 13/23 (56%), Frame = -1
Query: 35 VIFFNDNKPDPTYEILKQFQQAH 57
VIF N+ K T EIL F +H
Sbjct: 307268 VIFINNLKTIITSEILNSFI*SH 307200
Score = 40 (18.7 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 8/25 (32%), Positives = 13/25 (52%), Frame = -2
Query: 157 VNHLHYIQNNDQYIWNIVINTAFFK 181
V+ L + Q++W + INT K
Sbjct: 124057 VSKLQLNSSQPQFLWALRINTVVVK 123983
Score = 39 (18.2 bits), Expect = 4.0, Poisson P(2) = 0.98
Identities = 6/8 (75%), Positives = 7/8 (87%), Frame = -1
Query: 312 VFTNKHHG 319
+FTNK HG
Sbjct: 566297 IFTNKEHG 566274
Score = 39 (18.2 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 7/12 (58%), Positives = 9/12 (75%), Frame = -2
Query: 316 KHHGIYLLWVNR 327
KH YLL++NR
Sbjct: 270673 KHSPCYLLFINR 270638
Score = 39 (18.2 bits), Expect = 8.0, Poisson P(3) = 1.0
Identities = 6/18 (33%), Positives = 13/18 (72%), Frame = -3
Query: 321 YLLWVNRLKHFKKLVQAQ 338
+ L + ++KH++KL + Q
Sbjct: 513009 FQLKLEKIKHYRKLYEQQ 512956
Score = 39 (18.2 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 10/31 (32%), Positives = 17/31 (54%), Frame = -1
Query: 102 NCFKAIVETIQGENFDIAVLNSIVYLQMLKN 132
NC + V T++GEN ++ + +LKN
Sbjct: 217103 NCLFSRVITVEGENNVFKHIHVRIKCIVLKN 217011
Score = 38 (17.8 bits), Expect = 5.1e+02, Poisson P(9) = 1.0
Identities = 7/12 (58%), Positives = 10/12 (83%), Frame = -1
Query: 227 TQKNSIKLLSFI 238
T KN ++LLSF+
Sbjct: 364982 TLKNKLQLLSFV 364947
Score = 38 (17.8 bits), Expect = 5.1e+02, Poisson P(9) = 1.0
Identities = 7/13 (53%), Positives = 9/13 (69%), Frame = -1
Query: 31 NGVKVIFFNDNKP 43
NGVK + +ND P
Sbjct: 445796 NGVKFLIWNDIAP 445758
Score = 38 (17.8 bits), Expect = 5.1e+02, Poisson P(9) = 1.0
Identities = 7/21 (33%), Positives = 11/21 (52%), Frame = -2
Query: 261 NNKIESRFWRRQAFIWFSFIN 281
+ ++ + FW I F FIN
Sbjct: 104173 SGELNTIFWALSLLILFHFIN 104111
Score = 38 (17.8 bits), Expect = 16., Poisson P(4) = 1.0
Identities = 7/23 (30%), Positives = 14/23 (60%), Frame = -2
Query: 291 QTKSVLQKLFDFMEANGIYDRVF 313
Q + VL K ++ + N + +R+F
Sbjct: 511549 QYRLVLTKYYETINKNEVTERIF 511481
Score = 37 (17.3 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 6/14 (42%), Positives = 10/14 (71%), Frame = -1
Query: 61 AIEVHCGEKNVGVG 74
++++ CG NV VG
Sbjct: 553151 SLDIVCGANNVRVG 553110
Score = 37 (17.3 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 9/17 (52%), Positives = 10/17 (58%), Frame = -2
Query: 209 LWIDVNGVNYYLRPNSA 225
L ID VN Y PN+A
Sbjct: 194302 LCIDHPPVNPYFHPNNA 194252
Score = 37 (17.3 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 7/22 (31%), Positives = 14/22 (63%), Frame = -3
Query: 116 FDIAVLNSIVYLQMLKNDFLIK 137
F AV S++ + + +ND L++
Sbjct: 130275 FKTAVTPSLIAVAICRNDLLLQ 130210
Score = 37 (17.3 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 8/23 (34%), Positives = 13/23 (56%), Frame = -1
Query: 236 SFIEAYERLYFHLKKVGKLADFI 258
+F++ LY H K V +L + I
Sbjct: 528248 NFVKQSNNLYVHEKLVNELKENI 528180
Score = 36 (16.8 bits), Expect = 64., Poisson P(6) = 1.0
Identities = 6/14 (42%), Positives = 10/14 (71%), Frame = -3
Query: 234 LLSFIEAYERLYFH 247
LL+ ++ + LYFH
Sbjct: 423534 LLNVVDRLDDLYFH 423493
Score = 36 (16.8 bits), Expect = 5.1e+02, Poisson P(9) = 1.0
Identities = 7/16 (43%), Positives = 12/16 (75%), Frame = -2
Query: 47 YEILKQFQQAHPQLAI 62
+++ K F +A PQ+AI
Sbjct: 255670 FKLTKLFVKAGPQVAI 255623
Score = 36 (16.8 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 8/17 (47%), Positives = 12/17 (70%), Frame = -1
Query: 49 ILKQFQQAHPQLAIEVH 65
I KQF+ + +LAI +H
Sbjct: 273350 IQKQFRLMNCRLAIVIH 273300
Score = 36 (16.8 bits), Expect = 5.1e+02, Poisson P(9) = 1.0
Identities = 7/20 (35%), Positives = 12/20 (60%), Frame = -3
Query: 216 VNYYLRPNSASTQKNSIKLL 235
VN + PN+ +KNS+ +
Sbjct: 211503 VNRAIGPNTLLDKKNSLNFI 211444
Score = 36 (16.8 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 7/24 (29%), Positives = 13/24 (54%), Frame = -3
Query: 65 HCGEKNVGVGGSRDQLINYVDTPY 88
+C +V SR +N+ D+P+
Sbjct: 349572 YCSYVSVAFWQSRFLKVNHFDSPF 349501
Score = 36 (16.8 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 7/24 (29%), Positives = 14/24 (58%), Frame = -3
Query: 293 KSVLQKLFDFMEANGIYDRVFTNK 316
+++++ L F + + RV TNK
Sbjct: 298353 QNLIEYLVSFFKKQQLDQRVITNK 298282
Score = 36 (16.8 bits), Expect = 1.3e+02, Poisson P(7) = 1.0
Identities = 7/26 (26%), Positives = 14/26 (53%), Frame = -1
Query: 100 DPNCFKAIVETIQGENFDIAVLNSIV 125
D CF + E +F + ++NS++
Sbjct: 117248 DSCCFLGLTEPK*P*SFSLLIINSLI 117171
Score = 36 (16.8 bits), Expect = 2.6e+02, Poisson P(8) = 1.0
Identities = 8/28 (28%), Positives = 14/28 (50%), Frame = -2
Query: 273 AFIWFSFINVSWMKAEFEQTKSVLQKLF 300
+ IW +++ + E+ K L KLF
Sbjct: 287143 SMIWLKTVSIPAPLTKSEKFKRTLFKLF 287060
Score = 36 (16.8 bits), Expect = 1.3e+02, Poisson P(7) = 1.0
Identities = 10/32 (31%), Positives = 14/32 (43%), Frame = -1
Query: 103 CFKAIVETIQGENFDIAVLNSIVYLQMLKNDF 134
CF + V F +A S++ L NDF
Sbjct: 41279 CFFSCVFNNSESCFALASKKSVILGAFLTNDF 41184
Score = 35 (16.4 bits), Expect = 2.0, P = 0.86
Identities = 6/8 (75%), Positives = 8/8 (100%), Frame = -3
Query: 4 LISILVPC 11
L+SIL+PC
Sbjct: 24639 LLSILLPC 24616
Parameters:
E = 5.0, S = 47 (22.0 bits), E2 = 0.13, S2 = 35
W = 3, T = 13 (6.1 bits), X = 22 (10.3 bits)
M = BLOSUM62
C = 0 (Standard genetic code)
H = 0, V = 500, B = 250
-gapdecayrate 0.5 (the default)
Statistics:
Lambda = 0.324, Lambda/ln2 = 0.467, K = 0.141, H = 0.625 bits/position
Expected/Observed high score = 49 (22.9 bits) / 1011 (472.3 bits)
# of letters in query: 341 aa
# of neighborhood words in query: 3622
# of exact words scoring below T: 15
Database: MGseq
# of letters in database: 580,073 bp
# of word hits against database: 276,162
# of failed hit extensions: 230,829
# of successful extensions: 985
# of overlapping HSPs discarded: 936
# of HSPs reportable: 49
# of sequences in database: 1
# of db sequences with at least one HSP: 1
No. of states in DFA: 523 (52 KB)
Total size of DFA: 91 KB (128 KB)
Time to generate neighborhood: 0.03u 0.00s 0.03t Real: 00:00:00
No. of processors used: 1
Time to search database: 3.80u 0.05s 3.85t Real: 00:00:10
Total cpu time: 3.91u 0.13s 4.05t Real: 00:00:10