Ruprecht-Karls-Universität Heidelberg






Publications by the Knop Lab


search pubmed for current publications by M. Knop

Seamless gene tagging by endonuclease-driven homologous recombination. Khmelinskii, A., Meurer, M., Duishoev, N., Delhomme, N., Knop, M. PLoS One. 2011;6(8):e23794. Epub 2011 Aug 22.


TIPI - TEV protease-mediated induction of protein instability Taxis, C. / Knop, M (2011) in press

Exploiting Spore-autonomous Fluorescent Protein Expression to Quantify Meiotic Chromosome Behaviors in Saccharomyces cerevisiae. Thacker, D., Knop, M., Keeney, S. Genetics. 2011 Aug 11. [EPub ahead of print] PMID: 21840861 [PubMed - as supplied by publisher]

Quantitative fluorescnce imaging of protein diffusion and interaction in living cells. Capoulade J., Wachsmuth M., Hufnagel, L. Knop, M.. Nat Biotechnol. 2011 Aug7. doi:10.1038/nbt.1928 [EPub ahead of print]

Yeast cell morphology and sexual reproduction - A short overview and some considerations. Knop, M. (2011) in press

Genomic Promoter replacement cassettes to alter gene expression in the yeast Saccharomyces cerevisiae. Kaufmann, A. & Knop, M. (2011) MethodsMolBiol. 2011;765:275-94.

Artificial tethering to nuclear pores promotes partitioning of extrachromosomal DNA during yeast asymmetric cell division. Khmelinskii, A., Meurer, M., Knop, M. & Schiebel, E. Curr Biol. 2011 Jan 11;21(1):R17-8.

Segregation of yeast nuclear pores. Khmelinskii, A., Keller, P.J., Lorenz, H., Schiebel, E. & Knop, M. Nature. 2010 Jul 22;466(7305):E1.

Evolution of mutational robustness in the yeast genome: a link to essential genes and meiotic recombination hotspots. Keller, P.J. & Knop, M. PLoS Genet. 2009 Jun;5(6):e1000533. Epub 2009 Jun 26.

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron. Taxis, C., Stier, G., Spadaccini, R. & Knop, M. Mol Syst Biol. 2009;5:267. Epub 2009 Apr 28.

The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis. Maier, P., Rathfelder, N., Maeder, C.I., Colombelli, J., Stelzer, E.H.K. & Knop, M. EMBO J. 2008 Sep 17;27(18):2363-74. Epub 2008 Aug 28.

Reconstitution of Mdm2-dependent post-translational modifications of p53 in yeast. Di Ventura, B., Funaya, C., Antony, C., Knop, M. & Serrano, L. PLoS ONE. 2008 Jan 30;3(1):e1507.

Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Maeder, C.I., Hink, M.A., Kinkhabwala, A., Mayr, R., Bastiaens, P.I. & Knop, M. Nat Cell Biol. 2007 Nov;9(11):1319-26. Epub 2007 Oct 21.

Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. Maier, P., Rathfelder, N., Finkbeiner, M.G., Taxis, C., Mazza, M., Le Panse, S., Haguenauer-Tsapis, R. & Knop, M. EMBO J. 2007 Apr 4;26(7):1843-52. Epub 2007 Mar 8.

Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast. Taxis, C., Maeder, C., Reber, S., Rathfelder, N., Miura, K., Greger, K., Stelzer, E.H.K. & Knop, M. Traffic. 2006 Dec;7(12):1628-42.

Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis. Gordon, O., Taxis, C., Keller, P.J., Benjak, A., Stelzer, E.H.K., Simchen, G. & Knop, M. EMBO J. 2006 Aug 23;25(16):3856-68. Epub 2006 Aug 3.

Evolution of the hemiascomycete yeasts: on life styles and the importance of inbreeding. Knop, M. Bioessays. 2006 Jul;28(7):696-708.

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae. Taxis, C. & Knop, M. Biotechniques. 2006 Jan;40(1):73-8.

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system. Taxis, C., Keller, P., Kavagiou, Z., Jensen, L.J., Colombelli, J., Bork, P., Stelzer, E.H.K. & Knop, M. J Cell Biol 2005 Nov 21;171(4):627-40. Epub 2005 Nov 14.

Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis. Riedel, C.G., Mazza, M., Maier, P., Korner, R. & Knop, M. J Biol Chem 2005 Nov 11;280(45):37846-52. Epub 2005 Sep 7.

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization. Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B., Knop, M., Scheffzek, K. & Pepperkok, R. BMC Biol. 2005 Oct 7;3:21.

Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast. Knop, M., Miller, K.J., Mazza, M., Feng, D., Weber, M., Keranen, S. & Jantti, J. Mol Biol Cell 2005 Oct;16(10):4543-56. Epub 2005 Jul 19.

A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Janke, C., Magiera, M.M., Rathfelder, N., Taxis, C., Reber, S., Maekawa, H., Moreno-Borchart, A., Doenges, G., Schwob, E., Schiebel, E. & Knop, M. Yeast 2004 Aug;21(11):947-62.

Regulation of exocytotic events by centrosome-analogous structures. Taxis, C. & Knop, M. Topics Curr. Gen. 2004 10 193-207

Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. Maekawa, H., Usui, T., Knop, M. & Schiebel, E. EMBO J 2003 Feb 3;22(3):438-49.

Prospore membrane formation: how budding yeast gets shaped in meiosis. Moreno-Borchart, A.C. & Knop, M. Microbiol Res 2003;158(2):83-90.

Improved version of the red fluorescent protein (drFP583/DsRed/RFP). Knop, M., Barr, F., Riedel, C.G., Heckel, T. & Reichel, C. Biotechniques 2002 Sep;33(3):592, 594, 596-8 passim.

Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. Moreno-Borchart, A.C., Strasser, K., Finkbeiner, M.G., Shevchenko, A., Shevchenko, A. & Knop, M. EMBO J 2001 Dec 17;20(24):6946-57.

A screen for genes required for meiosis and spore formation based on whole-genome expression. Rabitsch, K.P., Toth, A., Galova, M., Schleiffer, A., Schaffner, G., Aigner, E., Rupp, C., Penkner, A.M., Moreno-Borchart, A.C., Primig, M., Esposito, R.E., Klein, F., Knop, M. & Nasmyth, K. Curr Biol 2001 Jul 10;11(13):1001-9.

Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis. Knop, M. & Strasser, K. EMBO J 2000 Jul 17;19(14):3657-67.

Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy. Pereira, G., Grueneberg, U., Knop, M. & Schiebel, E. EMBO J 1999 Aug 2;18(15):4180-95.

Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K. & Schiebel, E. Yeast 1999 Jul;15(10B):963-72.

Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Elliott, S., Knop, M., Schlenstedt, G. & Schiebel, E. Proc Natl Acad Sci U S A 1999 May 25;96(11):6205-10.

Microtubule organization by the budding yeast spindle pole body. Knop, M., Pereira, G. & Schiebel, E. Biol Cell 1999 May-Jun;91(4-5):291-304.

Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. Knop, M. & Schiebel, E. EMBO J 1998 Jul 15;17(14):3952-67.

Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body. Pereira, G., Knop, M. & Schiebel, E. Mol Biol Cell 1998 Apr;9(4):775-93.

Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p. Knop, M. & Schiebel, E. EMBO J 1997 Dec 1;16(23):6985-95.

The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. Knop, M., Pereira, G., Geissler, S., Grein, K. & Schiebel, E. EMBO J 1997 Apr 1;16(7):1550-64.

N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast. Knop, M., Hauser, N. & Wolf, D.H. Yeast 1996 Sep 30;12(12):1229-38.

The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment. Geissler, S., Pereira, G., Spang, A., Knop, M., Soues, S., Kilmartin, J. & Schiebel, E. EMBO J 1996 Aug 1;15(15):3899-911.

Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast. Knop, M., Finger, A., Braun, T., Hellmuth, K. & Wolf, D.H. EMBO J 1996 Feb 15;15(4):753-63.

Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast. Finger, A., Knop, M. & Wolf, D.H. Eur J Biochem 1993 Dec 1;218(2):565-74.

Vacuolar/lysosomal proteolysis: proteases, substrates, mechanisms. Knop, M., Schiffer, H.H., Rupp, S. & Wolf, D.H. Curr Opin Cell Biol 1993 Dec;5(6):990-6.


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