Ruprecht-Karls-Universitšt Heidelberg

Matthias Mayer
ZMBH Research Group Leader

ZMBH, Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Tel.: + 49-6221 54 6829
Fax.: +49-6221 54 5894
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M. Mayer's Annual Report

Welcome to the Mayer Lab!

Allosteric regulation of protein conformation

Many protein folding processes in the cell, in particular in signal transduction cascades controlling cell homeostasis, cell cycle and apoptosis, are dependent on the assistance by molecular chaperones of the Hsp70 and Hsp90 families. Thereby dynamic high molecular weight complexes are formed between the substrate proteins and the chaperones together with a number of co-chaperones. On one hand, the substrates are thus kept in an inactive state, on the other hand, the interaction with the chaperones is essential for subsequent activation of the substrate through the signaling cascades. The central question in this process is how Hsp70 and Hsp90 control the activity of their substrates; to which extent the chaperones influence the conformation of their substrates, how the chaperones prevent dimerization and nuclear localization and how they are able to modulate posttranslational modifications like phosphorylation.

To answer these questions we analyze the molecular mechanism of Hsp70 and Hsp90 chaperone systems. We investigate how the activity of the chaperones is regulated by co-chaperones and which conformational changes in chaperones and substrate proteins are necessary to reach the active state. For these studies we use biochemical and biophysical techniques including fluorescence spectroscopy, circular dichroism spectroscopy, surface plasmon resonance spectroscopy and amide hydrogen (1H/2H)-exchange in combination with high-resolution mass spectrometry.

Selected Publications

Kityk R, Kopp J, Mayer MP. Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones. Mol Cell (2018) 69:227-237.e4. doi: 10.1016/j.molcel.2017.12.003. (full paper).

Kityk, R., Vogel, M., Schlecht, R., Bukau, B. and Mayer, M.P. (2015) Pathways of allosteric regulation in Hsp70 chaperones. Nature Communications 6:8308.

Mayer, M. P. & Le Breton, L. (2015) Hsp90: Breaking the Symmetry. Molecular Cell. 58:8 20.

Mayer, M. P. (2013) Hsp70 chaperone dynamics and molecular mechanism. Trends in Biochemical Sciences. 38:507 514.

Kityk, R., Kopp, J., Sinning, I., & Mayer, M. P. (2012) Structure and dynamics of the ATP-bound open conformation of Hsp70 chaperones. Molecular Cell 48:863 874.

Lee, C.-T., Graf, C., Mayer, F. J., Richter, S. M., & Mayer, M. P. (2012) Dynamics of the regulation of Hsp90 by the co-chaperone Sti1. EMBO J 31:1518 1528.