Ruprecht-Karls-Universität Heidelberg






Publications by members of the Melchior lab

Search pubmed for publications by Frauke Melchior


Ritterhoff T, Das H, Hao Y, Sakin V, Flotho A, Werner A, Melchior F (2016). Reconstitution of the Recombinant RanBP2 SUMO E3 Ligase Complex.Methods Mol Biol 2016; 1475:41-54. (Abstract)

 

Stankovic-Valentin N, Drzewicka K, König C, Schiebel E, Melchior F (2016). Redox regulation of SUMO enzymes isrequired for ATM activity and survival in oxidative stress. EMBO J, pii: e201593404. (Abstract)

 

Ritterhoff, T., Das, H., Hofhaus, G., Schröder, R.R., Flotho, A., and Melchior, F. (2016). The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes. Nat Commun 7, 11482.(Abstract)

 

Sommer, S.1, Ritterhoff, T.1, Melchior, F. * and Mootz, H.D.*(2015). A stable chemical SUMO1-Ubc9 conjugate specifically binds as a thioester mimic to the RanBP2E3-ligase complex. ChemBioChem16, 1183-1189.1contributed equally.*co-corresponding authors.(Abstract).

Sakin, V., Richter, S.M., Hsiao, H.H., Urlaub, S., Melchior, F. (2015). Sumoylation of the GTPase Ran by the RanBP2 SUMO E3 Ligase Complex. J. Biol. Chem. 290(39):23589-602.(Abstract).

Stein S, Oosterveer MH, Mataki C, Xu P, Lemos V, Havinga R, Dittner C, Ryu D, Menzies KJ, Wang X, Perino A, Houten SM, Melchior F, Schoonjans K(2014). SUMOylation-Dependent LRH-1/PROX1 Interaction Promotes Atherosclerosis by Decreasing Hepatic Reverse Cholesterol Transport. Cell Metab 20(4):603-13.(Abstract).

Vorpahl, M., Schönhofer-Merl, S., Michaelis, C., Flotho, A., Melchior, F., et al. (2014). The Ran GTPase-Activating Protein (RanGAP1) Is Critically Involved in Smooth Muscle Cell Differentiation, Proliferation and Migration following Vascular Injury: Implications for Neointima Formation and Restenosis. PLos ONE 9(7):e101519.(Abstract)

Barysch, S.V., Dittner, C., Flotho, A., Becker, J., Melchior, F. (2014). Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies.Nat. Protoc. 9(4):896-909.
(Abstract).

Hutten S., Chachami G., Winter U., Melchior F., Lamond Al. (2014). A role for the CB-associated SUMO isopeptidase USPL1 in RNAPII-mediated snRNA transcription. J Cell Sci. 127(Pt5):1065-78. (Abstract).

Flotho, A and Melchior, F. (2013). Sumoylation: a regulatory protein modification in health and disease. Annu Rev Biochem 82, 357-85 (Abstract).

Becker, J., Barysch, S.V., Karaca, S., Dittner, C., Hsiao, H.H., Berriel Diaz, M., Herzig, S., Urlaub, S.,Melchior, F. (2013). Detecting endogenous SUMO targets in mammalian cells and tissues. Nat. Struct. Mol. Biol 20(4):525-531 (Abstract).

Werner A, Disanza A, Reifenberger N, Habeck G, Becker J, Calabrese M, Urlaub H, Lorenz H, Schulman B, Scita G, Melchior, F (2013). SCF(Fbxw5) mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression. Nat Cell Biol. 15(2):179-88.(Abstract)

Tirard, M., Hsiao, H.H., Nikolov, M., Urlaub, H., Melchior, F., Brose, N. (2012). In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice. PNAS, 109(51):21122-7. (Abstract)

Pilla E, Möller U, Sauer G, Mattiroli F, Melchior F, Geiss-Friedlander R (2012). A novel SUMO1-specific interacting motif in Dipeptidyl peptidase 9 (DPP9) that is important for enzymatic regulation. J Biol Chem. 287(53):44320-9. (Abstract)

Flotho A, Werner A (2012). The RanBP2/RanGAP1*SUMO1/Ubc9 complex: A multisubunit E3 ligase at the intersection of sumoylation and the RanGTPase cycle. Nucleus 3(5), 429-32. (Abstract)

Schulz, S., Chachami, G., Kozaczkiewicz, L., Winter, U., Stankovic-Valentin, N., Haas, P., Hofmann, K., Urlaub, H., Ovaa, H., Wittbrodt, J., Meulmeester, E., Melchior, F. (2012) Ubiquitin Specific Protease Like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO Rep., 13(10), 930-8. (Abstract)

Werner A, Melchior F (2012). SUMO unloads the Kap114 cab. EMBO J. 31, 2439-2440. (Abstract)

Brandl A, Wagner T, Uhlig KM, Knauer SK, Stauber RH, Melchior F, Schneider G, Heinzel T, Krämer OH (2012). Dynamically regulated sumoylation of HDAC2 controls p53 deacetylation and restricts apoptosis following genotoxic stress. J Mol Cell Biol. 4(5), 285-93. (Abstract)

Werner A, Flotho, A, Melchior, F. (2012). The RanBP2/RanGAP1*SUMO1/Ubc9 Complex Is a Multisubunit SUMO E3 Ligase. Mol Cell. 46, 287-298. (Abstract)

Flotho A, Werner A, Winter T, Frank AS, Ehret H and Melchior F (2012). Recombinant reconstitution of sumoylation reactions in vitro. Methods Mol Biol. 832, 93-110. (Abstract)

Krumova P, Meulmeester E, Garrido M, Tirard M, Hsiao HH, Bossis G, Urlaub H, Zweckstetter M, Kügler S, Melchior F, Bähr M, Weishaupt JH (2011). Sumoylation inhibits alpha-synuclein aggregation and toxicity. J Cell Biol. 194(1), 49-60. (Abstract)

Moutty, MC., Sakin, V., Melchior, F. (2011). Importin alpha/beta mediates nuclear import of individual SUMO E1 subunits and of the holo-enzyme. Mol Biol Cell. 22(5):652-60. (Abstract)

Splinter, D., Tanenbaum, M.E., Lindqvist, A., Jaarsma, D., Flotho, A., Yu, K.L., Grigoriev, I., Engelsma, D., Haasdijk, E.D., Keijzer, N. Demmers, J., Fornerod, M., Melchior, F., Hoogenraad, C.C., Medema, R.H., Akhmanova, A. (2010). Bicaudal D2, dynein and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry. PloS Biology, 8, e1000350. (Abstract)

Geiss-Friedlander, R., Parmentier, N., Moeller, U., Urlaub, H., van den Eynde, B.J. and Melchior, F. (2009). The cytoplasmic peptidase DPP9 is rate-limiting for degradation of proline-containing peptides. JBC 284, 27211-27219. (Abstract)

Hsiao, H.H., Meulmeester, E., Frank, B.T.C., Melchior, F., Urlaub, H. (2009). "ChopNspice", a mass-spectrometric approach that allows identification of endogenous SUMO-conjugated peptides. Mol Cell Proteomics 8(12), 2664-75. (Abstract)

Stankovic-Valentin, N., Kozaczkiewicz, L., Curth, K. and Melchior, F. (2009). An in vitro FRET-based assay for the analysis of SUMO conjugation and isopeptidase cleavage. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 241-251. (Abstract)

Werner, A., Moutty, M.C., Moeller, U. and Melchior, F. (2009). Performing in vitro sumoylation reactions using recombinant enzymes. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 187-199. (Abstract)

Knipscheer P, Flotho A, Klug H, Olsen JV, van Dijk WJ, Fish A, Johnson ES, Mann M, Sixma TK, Pichler A. (2008). Ubc9 sumoylation regulates SUMO target discrimination. Mol Cell. 31(3):371-82. doi: 10.1016/j.molcel.2008.05.022

 

Meulmeester, E. and Melchior, F. (2008). Cell Biology: SUMO. Nature 452, 709-711. (no Abstract available)

Meulmeester, E., Kunze, M., Hsiaoh, H.H., Urlaub, H., Melchior, F. (2008). Mechanism and consequences of paralog specific sumoylation of USP25. Mol Cell 30, 610-619. (Abstract)

Hutten, S., Flotho, A., Melchior, F. and Kehlenbach, R.H. (2008). The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear import. Mol. Biol. Cell 10, 2300-2310. (Abstract)

Geiss-Friedlander, R. and Melchior F. (2008). SUMOylation. Chapter in: Encyclopedia of Molecular Pharmacology (Eds.: S. Offermann and W. Rosenthal), Springer Verlag, Part 19, 1163-1167.

Geiss-Friedlander, R. and Melchior F. (2007). Concepts in SUMOylation: a decade on. Nat. Rev. Mol. Cell Biol. 8, 947-956. (Abstract) 

Tirard, M., Almeida, O.F.X., Hutzler, P., Melchior, F. and Michaelidis, T.M. (2007). Sumoylation and proteasomal activity determine the transactivation properties of the mineralcorticoid receptor. Mol. Cell. Endocrin., 268, 20-29. (Abstract)

Le Cam L, Linares LK, Paul C, Julien E, Lacroix M, Hatchi E, triboulet R, Bossis G, Shmueli A, Rodriguez MS, Coux O, Sardet C. (2006). E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation. Cell. Nov 17; 127(4):775-88.

 

Bossis, G. and Melchior, F. (2006). Regulation of SUMOylation by reversible oxidation of SUMO conjugating enzymes. Mol. Cell 21, 349-357. (Abstract)

Bossis, G. and Melchior, F. (2006). SUMOylation. Chapter in: Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. (Ed.: K. Ruckpaul and D. Ganten), Springer.

Bossis, G. and Melchior, F. (2006). SUMO: regulating the regulator. Cell Div. 1:13. (Abstract)

Tiefenbach, J., Novac, N., Eck, M., Melchior, F. and Heinzel, T. (2006). SUMOylation of the co-repressor N-CoR modulates its capacity to repress transcription. Mol. Biol. Cell 17, 1643-1651. (Abstract)

Bossis, G., Chmielarska, K., Gaertner, U., Pichler, A., Stieger, E., and Melchior, F. (2005). A FRET-based assay to study SUMO modification in solution. Methods in Enzymology. 398, 20-32. (Abstract)

Palacios, S., Perez, L.H., Welsch, S., Schleich, S., Chmielarska, K., Melchior, F., and Krijnse Locke, J. (2005). Mol. Biol. Cell Quantitative SUMO-1 modification of a vaccinia virus protein is required for its specific localization and prevents its self-association. Mol Biol. Cell 16, 2822-2835. (Abstract)

Pichler, A, Knipscheer, P., Oberhofer, E., van Dijk, W.J., Koerner, R., Olsen, J.V., Jentsch, S., Melchior, F., and Sixma, T.K. (2005). SUMO modification of the ubiquitin conjugating enzyme E2-25K. Nat. Struct. & Mol. Biol.12, 264-269. (Abstract)

Beck, M., Foerster, F., Ecke, M., Plitzko, J., Melchior, F., Gerisch, G., Baumeister, W., Medalia, O. (2004). Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography. Science, 306, 1387-1390. (Abstract) 

Pichler, A., Knipscher, P., Saitoh, H., Sixma, T., and Melchior, F. (2004). SUMO E3 ligase RanBP2 is neither Hect- nor Ring-type. Nat. Struct. & Mol. Biol. 11, 984 - 991. (Abstract)

Pichler, A., and Melchior, F. (2004). SUMO E3 ligases. Chapter in: SUMOylation. Molecular Biology and Biochemistry (Ed.: Van Wilson, G.), Horizon Press.

Melchior, F. and Pichler, A (2004). SUMO Modification, in Encyclopedia of Biological Chemistry (W.J. Lennarz & M.D. Lane, eds), Elsevier, Oxford, Vol.4, pp. 130-134.

Liang, M., Melchior, F., Feng, X.H., and Lin, X. (2004). Regulation of Smad4 sumoylation and transforming growth factor-beta signaling by protein inhibitor of activated STAT1. J. Biol. Chem. 279, 22857-22865. (Abstract)

Swaminathan, S., Kiendl, F., Koerner, R., Lupetti, R., Hengst, L. and Melchior, F. (2004). RanGAP1*SUMO-1 is phosphorylated at the onset of mitosis and remains associated with RanBP2 upon NPC disassembly. J. Cell Biol. 164, 965-971. (Abstract)

Lin, X., Sun, B., Liang, M., Liang, YY., Gast, A., Hildebrand, J., Brunicardi, F.C., Melchior, F., and Feng, X.H. (2003). Opposed Regulation of Corepressor CtBP by SUMOylation and PDZ Binding. Mol. Cell 11, 1389-1396. (Abstract)

Lin, X., Liang, M., Liang, Y.Y., Brunicardi, F.C., Melchior, F., Feng, X.H. (2003). Activation of Transforming Growth Factor-b signaling by SUMO-1 modification of tumor suppressor Smad4/DPC4. J. Biol. Chem. 278, 18714-18719. (Abstract)

Melchior, F., Schergaut, M., and Pichler, A. (2003). SUMO: ligases, isopeptidases and nuclear pores. Trends Biochem Sci. 28, 612-618. (Abstract)

Melchior F., (2003). Ubiquitin-verwandte Proteine. BIOspektrum 6, 691-693.

Connor, M.K., Kotchetkov, R., Cariou, S., Beniston, R.G., Resch, A., Lupetti, R., Melchior, F., Hengst, L., and Slingerland, J.M. (2003). CRM1/Ran-mediated nuclear export of p27Kip1 involves a nuclear export signal and links p27 export and proteolysis. Mol. Biol. Cell. 14, 201-213. (Abstract)

Sapetschnig, A., Rischitor, G., Braun, H., Doll, A., Schergaut, M., Melchior, F. and Suske, G. (2002). Transcription factor Sp3 is silenced through SUMO modification by PIAS1. EMBO J. 21, 5206-5215. (Abstract)

Kirsh, O., Seeler, J.S., Pichler, A., Gast, A, Mueller, S., Miska, E., Mathieu, M., Harel-Bellan, A., Kouzarides, T., Melchior, F. and Dejean, A. (2002). The SUMO E3 Ligase RanBP2 Promotes Modification of the HDAC4 Deacetylase. EMBO J. 21, 2682-2691. (Abstract)

Pichler, A. and Melchior, F. (2002). Ubiquitin-related modifier SUMO1 and nucleocytoplasmic transport. Traffic 3, 381-387. (Abstract)

Pichler, A., Gast, A., Seeler, J.S., Dejean, A. and Melchior, F. (2002). The nucleoporin RanBP2 is a SUMO1 E3 Ligase. Cell 108, 109-120. (Abstract)

Swaminathan, S. and Melchior, F. (2002). Calcium, viruses and nuclear pores: Meeting report from the EMBO workshop "Signal-transduction-mediated regulation of nuclear transport", Strasbourg, France, 11-14 August 2001. Traffic 3, 74-76. (no Abstract available)

Melchior, F. (2002). A decade's worth of Ran. Trends Cell Biol. 12, 203. (Abstract)

Melchior, F. and Hengst, L. (2002). SUMO-1 and p53, Cell Cycle 1(4), 1, 245-249. (Abstract)

Swaminathan, S. and Melchior, F. (2002). Nucleocytoplasmic transport: more than the usual suspects. Developmental Cell 3, 304-306. (Abstract)

Melchior, F. (2001). Ran GTPase cycle: One mechanism - two functions. Curr Biol., 11, R257-60. (Abstract)

Sachdev, S., Bruhn, L., Sieber, H., Pichler, A., Melchior, F. and Grossschedl, R. (2001). PIASy represses LEF1 activity by stimulating SUMO conjugation and targeting of LEF1 to nuclear bodies. Genes Dev., 15, 3088-103. (Abstract)

Melchior, F. (2000). SUMO-1 - Non-Classical Ubiquitin. Annu. Rev. Cell Dev. Biol.,16, 591-626. (Abstract)

Melchior, F. and Hengst, L. (2000). Mdm2-SUMO1: Is bigger better? Nature Cell Biol., 2, E161-E163. (no Abstract available)

Bayer, P., Andreas Arndt, A., Metzger, S., Mahajan, R., Melchior, F., Jaenicke, R., and Becker, J. (1998). Structure determination of the small ubiquitin-related modifier SUMO-1. J. Mol. Biol. 280, 275-286. (Abstract)

Melchior, F. and Gerace, L. (1998). Two-way trafficking with Ran? Trends Cell Biol. 8, 175-179. (Abstract)

Lee, G.W., Melchior, F., Matunis, M.J., Mahajan, R., Tian, Q., and Anderson, P. (1998). Modification of Ran GTPase-activating protein by the small ubiquitin-related modifier SUMO-1 requires Ubc9, an E2-type ubiquitin-conjugating enzyme homologue. J. Biol. Chem., 273, 6503-6507. (Abstract)

Mahajan, R., Gerace, L. and Melchior, F. (1998). The C-terminal domain of RanGAP1 contains the acceptor site for SUMO1 modification and is sufficient for targeting to the nuclear envelope. J. Cell Biol. 140, 259-270. (Abstract)

Melchior, F. (1998). Nuclear protein import in a permeabilized cell assay. in: Protein targeting protocols (Editor R. A. Clegg) Methods in Molecular Biology 88, 265-273. (no Abstract available)

Delphin, C., Guan, T., Melchior, F. and Gerace, L. (1997). RanGTP targets p97 to RanBP2 at the cytoplasmic periphery of the nuclear pore complex. Mol. Biol. Cell 8, 2379-2390. (Abstract)

Preisig-Mueller, R., Gehlert, R., Melchior, F., Stietz, U., and Kindl, H. (1997). Plant Polyketide Synthases leading to Stilbenoids have a domain catalyzing malonyl-CoA:CO2 exchange, malonyl-CoA decarboxylation, and covalent enzyme modification and a site for chain lengthening. Biochemistry 36, 8349-8358. (Abstract)

Mahajan, R., Delphin, C., Guan, T., Gerace, L. and Melchior, F. (1997). A small ubiquitin related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107. (Abstract)

Melchior, F., and Gerace, L. (1995). Mechanisms of nuclear protein import. Curr. Opin. Cell Biol. 7, 310-318. (Abstract)

Melchior, F., Guan, T., Yokoyama, N., Nishimoto, T., and Gerace, L. (1995). GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol. 131, 571-581. (Abstract)

Melchior, F., Sweet, D. J., and Gerace, L. (1995). Analysis of Ran/TC4 function in nuclear protein import. Methods Enzym. 257, 279-291. (Abstract)

Melchior, F., Guan, T., Paschal, B., and Gerace, L. (1995). Biochemical and structural analysis of nuclear protein import. Cold Spring Harbor Sym. Quant. Biol. LX, 707-716. (no Abstract available)

Becker, J., Melchior, F., Gerke, V., Bischoff, F. R., Ponstingl, H., and Wittinghofer, A. (1995). RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J. Biol. Chem. 270, 11860-11865. (Abstract)

Melchior, F., Paschal, B., Evans, J., and Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659. (Abstract)

Melchior, F., Weber, K., and Gerke, V. (1993). A functional homologue of the RNA1 gene product in Schizosaccharomyces pombe: Purification, biochemical characterization, and identification of a Leucine-rich Repeat motif. Mol. Biol. Cell. 4, 569-581. (Abstract)

Doherty, N. M., Hogarth, G., Knox, S. A. R., Macpherson, K. A., Melchior, F., Morton, D. A. V. and Orpen, A. G. (1992). Phosphorus-Carbon bond cleavage at a di-iron centre: synthesis of m-phosphidomethyl complexes [Fe2(CO)6(m-CH2PR2)(m-PR2)] from [Fe2(CO)6(m-CO)(m-R2PCH2PR2)]. Inorganica Chimica Acta, 198-200, 257-270. (Abstract)

Melchior, F., and Kindl, H. (1991). Coordinate and elicitor-dependent expression of Stilbene Synthase and Phenylalanine Ammonia-Lyase genes in Vitis cv. Optima. Arch. Biochem. Biophys. 288, 552-557. (Abstract)

Liswidowati, Melchior, F., Hohmann, F., Schwer, B., and Kindl, H. (1991). Induction of Stilbene Synthase by Botrytis cinerea in cultured Grapevine cells.  Planta 183, 307-314. (Abstract)

Melchior, F., and Kindl, H. (1990). Grapevine Stilbene Synthase cDNA only slightly differing from Chalcone Synthase cDNA is expressed in Escherichia coli into a catalytically active Enzyme. FEBS Lett. 268, 17-20. (Abstract)

Doherty, N. M., Hogarth, G., Knox, S. A. R., Macpherson, K. A., Melchior, F., and Orpen, A. G. (1986). Phosphorus-Carbon bond cleavage at a di-iron centre. Conversion of m-R2PCH2PR2 to m-R2PCH2 and m-PR2: Crystal structures of [Fe2(CO)4(m-Ph2PCH2)(m-PPH2)(m-Me2PCH2PMe2)] and [Fe(CO)6(m-PhPCH(Me)P(Ph)(C6H4-o))]. J. Chem. Soc., Chem. Commun., 540-542. (Abstract)



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