Publications of the last 5 years


Lutz, R. and Bujard, H. (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucl. Acids Res. 25, 1203-1210

Freundlieb, S., Baron, U., Bonin, A.L., Gossen, M. and Bujard, H. (1997) Use of tetracycline controlled gene expression systems to study the mammalian cell cycle. Methods Enzymol. 283, 159-172.

Freundlieb, S., Baron, U. and Bujard, H. (1997) Controlling gene activities via the tet regulatory systems. Cell Biology - A Laboratory Handbook, 2nd ed., editor: Julio E. Celis, Vol 4, 230-238, Academic Press, San Diego, USA.

Baron, U., Gossen, M. and Bujard, H. (1997) Tetracycline controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucl. Acids Res. 25, 2723-2729.

Ohta, N., Iwaki, K., Itoh, M., Fu, J., Nakashima, S., Hato, M., Tolle, R., Bujard, H., Saitoh, A. and Tanabe, K. (1997) Epitope analysis of human T-cell response to MSP-1 of Plasmodium falciparum in malaria-nonexposed individuals. Int. Arch. Allergy Immunol. 114, 15-22.

Tremblay, P., Meiner, Z., Galou, M., Heinrich, C., Petromilli, C., Lisse, T., Cayateno, J., Torchia, M., Mobley, W., Bujard, H., DeArmond, S.J. and Prusiner, S.B. (1998) Doxycycline control of prion protein transgene expression modulates prion disease in mice. Proc. Natl. Acad. Sci. USA 95, 12580-12585

Baron, U., Schnappinger, D., Helbl, V., Gossen, M., Hillen, W. and Bujard, H. (1999) Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes. Proc. Natl. Acad. Sci. USA 96, 1013-1018

Freundlieb, S., Schirra-Müller, C. and Bujard, H. (1999) A tetracycline controlled activation/repression system for mammalian cells. J. Gene Med. 1, 4-12

Pan, W., Ravot, E., Tolle, R., Frank, R., Mosbach, R., Türbachova, I. and Bujard, H. (1999) Vaccine candidate MSP-1 from Plasmodium falciparum: a redesigned 4917 bp polynucleotide enables synthesis and isolation of full length protein from E.coli and mammalian cells. Nucl. Acids Res. 27, 1094-1103.

Redfern, C.H., Coward, P., Degtyarev, M.Y., Lee, E.K., Kwa, A., Hennig-hausen, L., Bujard, H., Fishman, G.I. and Conklin, B.R. (1999) In vivo conditional expression and signaling of a specifically designed Gi-coupled receptor. Nature Biotech. 17, 165-169.

Burghaus, P.A., Gerold, P., Pan, W., Schwarz, R.T., Lingelbach, K. and Bujard, H. (1999) Analysis of recombinant merozoite surface protein-1 of Plasmodium falciparum expressed in mammalian cells. Mol. Biochem. Parasitol. 104, 171-183.

Baron, U. and Bujard, H. (2000) The Tet repressor-based system for regulated gene expression in eukaryotic cells: principles and advances.
Methods Enzymol. 327, 659-686.

Urlinger, S., Baron, U., Thellmann, M., Hasan, M., Bujard, H. and Hillen, W. (2000) Exploring the sequence space for tetracycline dependent transcriptional activators: novel mutations yield expanded range and sensitivity.
Proc. Natl. Acad. Sci. USA 97, 7963-7968.

Lavon, I., Goldberg, I., Amit, S., Landsman, L., Jung, S., Tsuberi, B., Barshack, I., Kopolovic, J., Galun, E., Bujard, H. and Ben-Neriah, Y. (2000)
High susceptibility to bacterial infection, but no liver dysfunction, in mice compromised for hepatocyte NF-kB activation. Nature Med. 6, 573-577.

Mansuy, I. M. and Bujard, H. (2000) Tetracycline-regulated gene expression in the brain. Curr. Op. Neurobiol. 10, 593-596.

Hasan, M.T., Schönig, K., Graewe, W. and Bujard, H. (2001) Long-term, non-invasive imaging of regulated gene expression in living mice. Genesis 29, 116-122.

Verhoef, K., Marzio, G., Bujard, H. and Berkhout, B. (2001) Strict control of human immunodeficiency virus type 1 replication by a genetic switch: Tet for Tat. J. Virol. 75, 979-987.

Gossen, M. and Bujard, H. (2001) Tetracyclines in the control of gene expression in eukaryotes. In: Tetracyclines as molecular probes for micro and mammalian biology. ed. M. Nelson, W. Hillen, R.A. p. 139-157, Greenwald. Birkhäuser Verlag, Basel.

Lutz, R., Lozinski, T., Ellinger, T. and Bujard, H. (2001) Dissecting the functional program of Escherichia coli promoters: the combined mode of action of Lac repressor and AraC activator. Nucl. Acids Res. 29, 3873-3881.

Hess, J., Nielsen, P.J., Fischer, K.-D., Bujard, H. and Wirth, T. (2001) The B lymphocyte-specific coactivator BOB.1/OBF.1 is required at multiple stages of B cell development. Mol. Cell. Biol. 21, 1531-1539.

Meissner, M., Brecht, S., Bujard, H. and Soldati, D. (2001) Modulation of myosin A expression by a newly established tetracycline repressor based inducible system in Toxoplasma gondii. Nucl. Acids Res. 29, E115

Schönig, K. and Bujard, H. (2002) Generating conditional mouse mutants via tetracycline controlled gene expression. In: "The Transgenic Mouse: Methods and Protocols", eds. Jan van Deursen and Marten Hofker, p. 69-104, Humana Press Inc., USA.

Aurisicchio, L., Bujard, H., Hillen, W., Cortese, R., Ciliberto, G., La Monica, N. and Palombo, F. (2002) Regulated and prolonged expression of mIFNa in immuno-competent mice mediated by a helper-dependent adenovirus vector.
Gene Therapy 8, 1817-1825.

Vigna, E., Cavalieri, S., Ailles, L., Geuna, M., Loew, R., Bujard, H. and Naldini, L. (2002) Robust and efficient regulation of transgene expression in vivo by improved tetracycline dependent lentiviral vectors. Mol. Ther. 5, 252-261.

Lamartina, S., Roscilli, G., Rinaudo, C.D., Sporeno, E., Silvi, L, Hillen, W., Bujard, H., Cortese, R., Ciliberto, G. and Toniatti, C. (2002) Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators. Hum. Gene Ther. 13, 199-210.

Gossen, M. and Bujard, H. (2002) Studying gene function in eukaryotes by conditional gene inactivation. In Annual Review of Genetics, vol. 36, Annual Reviews, Palo Alto, USA, in press


Structure of the group

Tet regulatory systems



Summary of the current research activities