Ruprecht-Karls-Universität Heidelberg

Publications by the Clayton Lab

Search pubmed for publications by Christine Clayton


 Refereed research papers

Corsini, C.A., Clayton, C.E., Askonas, B.A. and Ogilvie, B.M. (1977) Suppressor cells and loss of B-cell potential in mice infected with  Trypanosoma bruceiClin. Exp. Immunol. 29, 122-131.

Clayton, C.E. (1978) Trypanosoma brucei:  influence of host strain and parasite antigenic type on infections in mice. Exp. Parasitol.  44,  202-208.

Mayor-Withey, K.S., Clayton, C.E., Roelants G.E. and Askonas, B.A. (1978)  Trypanosomiasis leads to extensive proliferation of B, T, and Null cells in spleen and bone marrow. Clin. Exp. Immunol. 34, 359-363.

Askonas, B.A., Corsini, C.A., Clayton, C.E. and Ogilvie, B.M. (1979) Functional depletion of T- and B- memory cells and other lymphoid cell subpopulations during trypanosomasis. Immunology 36, 313-321.

Clayton, C.E., Ogilvie, B.M. and Askonas, B.A. (1979) Trypanosoma brucei infection in nude mice:  B lymphocyte function is suppressed in the absence of T-lymphocytes. Parasite Immunol. 1, 39-48.

Clayton, C.E., Sacks, D.L., Ogilvie, B.M. and Askonas, B.A. (1979)   Membrane fractions of trypanosomes mimic the immunosuppressive and mitogenic effects of living parasites in the host. Parasite Immunol.  1, 241-249.
Clayton, C.E. (1980) In vitro immunity to Trypanosoma (Herpetosoma) musculiTrans. Roy. Soc. Trop. Med. Hyg. 74, 125.

Clayton, C.E., Selkirk, M.E., Corsini, C.A., Ogilvie, B.M., Askonas, B.A. (1980)  Murine trypanosomiasis:  cellular proliferation and functional depletion in the blood, peritoneum and spleen related to changes in bone marrow stem cells. Inf. Immun. 28, 824-831.

Clayton, C.E. and Rigby, P.W.J. (1981)  Cloning and characterization of the integrated viral DNA from three lines of SV40-transformed mouse cells. Cell 25, 547-559.

Lovett, M., Clayton, C.E., Murphy, D., Rigby, P.W.J., Smith, A.E. and Chaudry, F. (1982)  Structure and synthesis of a Simian Virus 40 super T-antigen. J. Virol. 44, (3) 963-973.

Clayton, C.E., Lovett, M. and Rigby, P.W.J. (1982)  Functional analysis of a Simian Virus 40 super-T-antigen. J. Virol. 44 (3) 974-982.

Clayton, C.E., Murphy, D., Lovett, M. and Rigby, P.W.J. (1982)  A fragment of SV40 T-antigen transforms. Nature 229, 59-61.

Zieg, J., Clayton, C.E., Ardeshir, F., Giulotto, E., Swyryd, E. and Stark, G.R. (1983)  Properties of single step mutants of syrian hamster cell lines resistant to N-(phosphonacetyl)-L-asparate. Mol. Cell. Biol. 3, 2089-2098.

Bhasin, V.K., Clayton, C.E., Trager, W., Cross, G.A.M. (1985)   Variations in the organization of repetitive DNA sequences in the genomes of Plasmodium falciparum clones. Mol. Biochem. Parasitol. 15, 149-158.

Clayton, C.E. (1985)  Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei. EMBO J. 4, 2997-3003.

Mowatt, M.R. and Clayton, C.E. (1987)  Developmental regulation of a novel repetitive protein of Trypanosoma brucei. Mol. Cell. Biol. 7, 2838-2844.

Clayton, C.E. (1987) Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei. J. Cell. Biol. 105, 2649-2654.

Clayton, C.E.  (1988)  Most proteins, including fructose bisphosphate aldolase,  are stable in the procyclic trypomastigote form of Trypanosoma bruceiMol. Biochem. Parasit. 28, 43-46.

Mowatt, M.R. and Clayton, C.E. (1988)  Polymorphism in the procyclic acidic repetitive protein gene family of Trpanosoma brucei. Mol. Cell. Biol. 8, 4055-4062.

Clayton,  C.E. and Fox,  J.E.  (1989)  Phosphorylation of fructose bisphosphate aldolase in Trypanosoma brucei. Mol. Biochem. Parasitol. 33, 73-80

Mowatt, M.R.,  Wisdom, G.S. and Clayton, C.E.  (1989) Variation of tandem repeats in the developmentally regulated Procyclic Acidic Repetitive Proteins of Trypanosoma brucei   Mol. Cell. Biol. 9, 1332-1335

Clayton,  C.E. and Mowatt,  M.R. (1989) The Procyclic Acidic Repetitive Protein of  Trypanosoma brucei.:  purification and post-translational modification. J. Biol. Chem. 264, 15088-15093.

Clayton, C.E.,  Fueri, J.P.,  Itzhaki, J.E.,  Bellofatto, V.  Wisdom, G.S.,  Vijayasarathy, S. and Mowatt, M.R. (1990) Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei. Mol. Cell. Biol 10, 3036-3047.

Vijayasarathy, S., .Ernest, I., Itzhaki, J. E.,.Sherman, D., Mowatt, M. R., Michels, P.A.M and Clayton, C.E. (1990) The genes encoding fructose bisphosphate aldolase in Trypanosoma brucei are interspersed with unrelated genes. Nucleic Acids Res. 18, 2967-2975

Zomerdijk, J.C.B.M., Ouellette, M., ten Asbroek, A.L.M.A., Kieft, R., Bommer, A.M.M., Clayton, C.E. and Borst, P. (1990) The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J. 9, 2791-2801.

Fung, K. and Clayton, C.E. (1991) Recognition of a peroxisomal tripeptide entry signal by the glycosomes of Trypanosoma brucei. Mol. Biochem. Parasitol. 45 261-264.

Sherman, D., Janz, L., Hug, M. and Clayton, C.E. (1991) Anatomy of the Procyclic Acidic Repetitive Protein promoter of Trypanosoma brucei. EMBO J  10 3379-3386.

Blattner, J., Swinkels, B., Dörsam, H., Prospero, T., Subramani, S., and Clayton, C.E.. (1992) Glycosome assembly in trypanosomes: variations in the acceptable degeneracy of a C-terminal microbody targetting signal. J. Cell Biol. 119, 1129-1136

Hug, M., Carruthers, V., Sherman, D., Hartmann, C., Cross, G.A.M., and Clayton, C.E.  (1993) A possible role for the 3'-untranslated region in developmental regulation in Trypanosoma brucei. Mol. Biochem. Parasitol. 61, 87-96
Janz, L., Hug, M. and C.E. Clayton  (1994) Factors that bind to RNA polymerase I promoter sequences of Trypanosoma bruceiMol. Biochem. Parasitol. 65, 99-108.

Janz, L. and Clayton, C.E. (1994) The PARP and rRNA promoters of Trypanosoma brucei are composed of dissimilar sequence elements that are functionally interchangeable. Mol. Cell. Biol. 14, 5804-5811

Wirtz, L.E. and Clayton, C.E. (1994) Reporter gene expression mediated by bacteriophage T7 and T3 RNA polymerases in Trypanosoma brucei. Nucleic Acids Res. 19, 3887-3894.

Hug, M. Hotz, H.-R., Hartmann, C. and Clayton, C.E. (1994) Hierarchies of RNA processing signals in a trypanosome RNA polymerase I transcription unit.  Mol. Cell. Biol. 14, 7428-7435

Blattner, J., Dörsam, H. and Clayton, C.E. (1995) N-terminal signal sequences mediate glycosomal import in trypanosomes. FEBS Lett. 360, 310-314

Wirtz, L.E. and Clayton, C.E. (1995) Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 268, 1179-1183.

Blattner, J. and Clayton, C.E. (1995) The 3'-untranslated regions from the Trypanosoma brucei  phosphoglycerate kinase genes mediate developmental regulation. Gene 162, 153-156.

Hotz, H.-R.,  Lorenz, P., Fischer, R., Krieger, S., and Clayton, C. E. (1995) Role of 3’-untranslated regions in developmental regulation of hexose transporter mRNAs in Trypanosoma brucei. Mol. Biochem. Parasitol. 75, 1-14.

Häusler, T.,  Stierhof, Y.D.,Wirtz, E., Clayton, C.E.  (1996) Import of a DHFR-hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterin. J. Cell Biol. 132, 311-324.

Häusler, T. and Clayton, C.E.  (1996) Post-transcriptional control of hsp70 mRNA in Trypanosoma brucei: Mol. Biochem. Parasitol. 76, 57-72.

Biebinger, S.., Rettenmaier, S., Flaspohler, J., Hartmann, C., Pena-Diaz, J., Wirtz, L.E., Hotz, H.R., Barry, J.D.,  Clayton, C.E. (1996) The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context. Nucleic Acids Res. 24, 1202-1211

Biebinger, S. and Clayton, C.E. (1996) A plasmid shuttle vector bearing an rRNA promoter is extrachromosomally maintained in Crithidia fasciculata. Exp. Parasitol. 83, 252-258

Biebinger, S.,  Wirtz, L. E., Lorenz, P., and Clayton, C.E. (1997) Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei. Mol. Biochem. Parasitol. 85, 99-112

Häusler, T., Stierhof, Y.-D. Blattner, J. and Clayton, C.E. (1997) Conservation of mitochondrial signal sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eucaryotes Crithidia, Trypanosoma and Trichomonas. Eur J. Cell Biol. 73, 240-251.

Hotz, H.-R., Hug, M., Huober, K., Hartmann, C. and Clayton, C. E. (1997) Mechanisms of developmental regulation in Trypanosoma brucei: A polypyrimidine tract in the 3'-untranslated region of a trypanosome surface protein mRNA affects RNA abundance and translation. Nucleic Acids Res. 25, 3017-3025

Lorenz, P., Meier, A., Erdmann, R., Baumgart, E. and Clayton, C.E. (1998) Elongation and clustering of glycosomes in Trypanosoma brucei overexpressing the glycosomal Pex11p.  EMBO J. 17, 3542-3555.

Hartmann, C., Hotz, H.-R., McAndrew, M. and Clayton, C.E. (1998) Effect of multiple downstream splice sites on polyadenylation in Trypanosoma brucei. Mol. Biochem. Parasitol. 93, 149-152.

McAndrew, M., Graham, S. and Clayton, C.E. (1998) Testing promoter activity in the trypanosome genome: isolation of a metacyclic-type VSG promoter, and unexpected insights into RNA polymerase II transcription. Exp. Parasitol. 90, 65-76.

Blattner, J., Helfert, S., Michels, P., and Clayton, C. E. (1998) Compartmentation of phosphoglycerate kinase in Trypanosoma brucei plays a critical role in parasite energy metabolism. Proc. Natl. Acad. Sci. USA  95, 11596-11600.

Ansorge, I., Melville, S., Hartmann, C. Steverding, D. and Clayton,C.E. (1999) Transcription of "inactive" expression sites in African trypanosomes leads to expression of multiple transferrin receptor variants in bloodstream forms. Mol. Biochem. Parasitol. 101, 81-94.

Drozd, M. and Clayton, C.E. (1999) Conformation of a regulatory 3'-untranslated region from Trypanosoma brucei. RNA  5, 1632-1644

Clayton, C.E.,  Ha, S.,  Rusche, L., Hartmann, C.,  Beverley, S. M. (2000) Tests of heterologous promoters and intergenic regions in Leishmania major Mol. Biochem. Parasitol. 105, 163-167..

Krieger, S., Schwarz, W., Ariyanagam, M.R., Fairlamb, A., Krauth-Siegel, L., and Clayton, C. E. (2000) Trypanosomes lacking trypanothione reductase are avirulent and show increased sensitivity to oxidative stress. Mol. Microbiol. 35, 542-552.

Ding, M., Clayton, C. and Soldati, D. (2000) Toxoplasma gondii catalase: are there peroxisomes in Toxoplasma? J. Cell Sci., 113, 2409-2419.

van Duersen, F.J., Shahi S.H., Turner C.M.R., Hartmann C., Guerra-Giraldez C., Matthews K.R. and Clayton,C.E. (2001) Characterisation of the growth and differentiation in vivo and in vitro of bloodstream-form Trypanosoma brucei strain TREU 927. Mol Biochem Parasitol.112, 163-172.

Maier, A., Schulreich, S., Bremser, M. and Clayton, C.E. (2001) Binding of coatomer by the PEX11 C-terminus is not required for function. FEBS Lett. 484, 82-86.

Maier, A., Lorenz, P., and Clayton, C.E. (2001) A essential dimeric membrane protein of trypanosome glycosomes. Mol. Microbiol. 39, 1443-1451

Maier, A., Bremser, M., Ding, M. and Clayton, C.E. (2001) The coatomer of Trypanosoma brucei. Mol. Biochem. Parasitol. 115, 55-61

Helfert, S., Estévez, A., Bakker, B., Michels, P.A.M. and Clayton C.E. (2001) The roles of triosephosphate isomerase and aerobic metabolism in Trypanosoma brucei. Biochem. J. 357, 117-125

Estevez, A., Kempf, T. and Clayton, C.E. (2001) The exosome of Trypanosoma brucei. EMBO J. 14, 3831-3839
Irmer, H. and Clayton, C.E. (2001) Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region. Nucleic Acids Res. 29, 4707-4715

Shahi, S., Krauth-Siegel, L. and Clayton, C.E. (2002) Overproduction of the putative thiol conjugate transporter TbMRPA causes melarsoprol resistance in Trypanosoma brucei. Mol. Microbiol. 43, 1129-1138

Drozdz, M., Quijada, L. and Clayton, C.E. (2002) RNA interference using independent sense and antisense plasmids Mol. Biochem. Parasitol. 121, 149-152

Drozdz, M., Palazzo, S.S., Salavati, R., Igo, R.P., O'Rear, J., Clayton, C. and Stuart, K. (2002) TbMBP81 is associated with RNA editing in Trypanosoma brucei. EMBO J. 21, 1791-1799.

Guerra-Giraldez, C., Quijada, L., Clayton, C.E. (2002) Compartmentation of enzymes in a microbody, the glycosome, is essential in Trypanosoma bruceiJ. Cell Sci. 115, 2651.

Diehl, S, Diehl, F, El-Sayed, N.M, Clayton, C.E,. Hoheisel, J.D. (2002) Analysis of stage-specific gene expression in the bloodstream and the procyclic form of Trypanosoma brucei using a genomic DNA microarray Mol. Biochem. Parasitol. 123, 115-123

Schmidt, A., Clayton, C.E., Krauth-Siegel,. R.L. (2002) Silencing of the thioredoxin gene in Trypanosoma brucei brucei. Mol. Biochem. Parasitol. 125, 207-210

Quijada, L., Guerra-Giraldez, C., Drozdz, M., Hartmann, C., Ding, M., and Clayton, C.E. (2002) Expression of the human RNA-binding protein HuR in Trypanosoma brucei induces differentiation-related changes in the abundance of developmentally-regulated mRNAs. Nucleic Acids Res. 30, 4414-4424

Mußmann, R., Janssen, H., Calafat, J., Engstler, M., Ansorge, I., Helfert, S., Clayton, C. and Borst, P. (2003) The expression level determines the surface distribution of the transferrin receptor in Trypanosoma brucei Mol. Microbiol. 47, 23-35

Estévez, A.M., Lehner, B., Sanderson, C.M., Ruppert, T. and Clayton, C. (2003) The roles of inter-subunit interactions in exosome stability. J. Biol. Chem. 278, 34943-34951.

Voncken, F., van Hellemond, J.J., Pfisterer, I., Maier, A., Hillmer, S. and Clayton, C. (2003) Depletion of GIM5 causes cellular fragility, a decreased glycosome number and reduced levels of ether-linked phospholipids in trypanosomes. J. Biol. Chem. .278, 35299-35310.

Biebinger, S., Helfert, S., Steverding, D., Ansorge, I and Clayton, C. (2003) Impaired dimerization and trafficking of ESAG6 lacking a glycosyl-phosphatidylinositol anchor Mol. Biochem. Parasitol. 132, 93-96

Haile, S. Estévez, A.M. and Clayton, C. (2003) A role for the exosome in the initiation of degradation of unstable mRNAs RNA 9, 1491-1501

Ding, M., Kwok, L.-U., Schluter, D., Clayton, C. and Soldati, D. (2003) The antioxidant systems in Toxoplasma gondii  and the role of cytosolic catalase in defense against oxidative injury. Mol. Microbiol. 51, 47-61 and vol 58, 915

Estévez, A.M., Haile, S., Steinbüchel, M., Quijada, L. and Clayton, C. (2004) Effects of depletion and overexpression of the Trypanosoma brucei ribonuclease L inhibitor homologue. Mol. Biochem. Parasitol. 133,137-41

Alibu, V.P., Storm, L., Clayton, C. and Horn, D. (2005) A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei . Mol. Biochem. Parasitol., 139, 75-82.

Brems, S., Guilbride, D.L., Gundlesdodjir-Planck, D., Bushold, C., Luu, V.-D., Schanne, M., Hoheisel, J. and Clayton, C.. (2005) The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes. Mol. Biochem. Parasitol. 139. 163-172.

Benz, C., Nilsson, D., Björn Andersson, B., Clayton, C. and Guilbride, D.L.. (2005) Messenger RNA processing sites in Trypanosoma brucei. Mol. Biochem. Parasitol. 143, 1235-1340.

Ivens, A.C., Clayton C. et al. (2005) The genome of the kinetoplastid parasite, Leishmania major. Science 309, 436-442.

Schlecker, T., Schmid, A., Dirdjaja, N., Voncken, F., Clayton, C., Krauth-Siegel, R.L. (2005) Substrate specificity, localization, and essential role of the glutathione peroxidase-type tryparedoxin peroxidases in Trypanosoma brucei. J. Biol. Chem 280, 14385-14394

De Gaudenzi JG, Frasch ACC, Clayton C. (2005) RNA-binding domain proteins in Kinetoplastids: a comparative analysis. Eukaryot Cell 4, 2106-2114

Alibu. V.P.,  Richter, C., Voncken, F., Shahi, S., Renggli, C.,  Brun, R., Clayton, C. (2006) The role of Trypanosoma brucei MRPA in melarsoprol susceptibility Mol. Biochem. Parasitol. 146, 38-44.

Colasante, C., Alibu, V.P., Kirchberger, S., Clayton, C.E., Tjaden, J. and Voncken, F. (2006) Characterisation and developmentally regulated localisation of metabolite carrier protein homologue MCP6 from Trypanosoma brucei Eukaryot Cell 5, 1194-1205

Li, C.-H., Irmer, H., Gudjonsdottir-Planck, D., Freese, S., Salm, H., Haile, S., Estévez, A. and Clayton, C.E. (2006) Role of a Trypanosoma brucei 5'->3' exoribonuclease homologue in mRNA degradation. RNA 12, 2171-2186. 10.1261/rna.291506.

Luu, V.D., Brems, S., Hoheisel, J. and Clayton, C. (2006) Functional analysis of Trypanosoma brucei PUF1. Mol Biochem. Parasitol 150, 340-349.

Haile, S., Cristodero, M., Clayton, C and Estévez, A. (2007) The subcellular localisation of trypanosome RRP6 and its association with the exosome. Mol Biochem. Parasitol 151, 52-58.

Colasante, C., Robles, A., Li, C.-H., Schwede, A., Benz, C., Voncken, F., Guilbride, D.L. and Clayton, C. (2007) Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei Mol. Biochem. Parasitol. 151, 193-204.

Benz, C and Clayton, C.E. (2007) The cyclin F box protein CFB2 is required for cytokinesis in  Trypanosoma brucei Mol. Biochem. Parasitol. 156, 217-224.

Hartmann, C., Benz, C., Brems, S., Ellis, L., Luu, V.D., Stewart, M., D'Orso, I., Bushold, C., Fellenberg, K.,  Frasch, A.C.C., Carrington, M., Hoheisel, J., and Clayton, C.E. (2007) The small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes Eukaryot. Cell. 6, 1964-78

Cristodero, M, Estévez, A and Clayton, C.E. (2007) Trypanosome MTR4 is involved in ribosomal RNA processing.Nucleic Acids Res.  35, 7023-30

Robles, A and Clayton C.E. (2008) Regulation of an amino acid transporter mRNA in Trypanosoma brucei. Mol. Biochem. Parasitol. 157, 102-106

Hartmann, C and Clayton, C. (2008) Regulation of a transmembrane protein gene family by the small RNA binding proteins UBP1 and UBP2.Mol. Biochem. Parasitol. 157, 112-115

Haanstra, JR, Stewart, M, Luu, V-D., van Tuijl, A., Westerhoff, H.V., *Clayton, C.E. and *Bakker, B.M. (2008). Control and regulation of gene expression: quantitative analysis of the expression of phosphoglycerate kinase in bloodstream-form Trypanosoma brucei. *joint senior authors. J. Biol. Chem.  283, 2495-2507

Cristodero, M., Böttcher, B., Diepholz, M., Scheffzeck, K. and Clayton, C. (2008) The exosome of Leishmania tarentolae: purification and structural analysis by electron microscopy. Mol. Biochem. Parasitol. 159, 24-29.

Schwede, A, Ellis, L, Luther, J., Carrington, M., Stoecklin, G. and Clayton, C.E. (2008) A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells. Nucleic Acids Res. 36, 3374-3388

Kramer, S., Queiroz, R., Ellis, L., Webb, H., Hoheisel, J., Clayton, C and Carrington, M. (2008) Stress granules and the heat shock response in Trypanosoma brucei J. Cell Sci. 121, 3002-3014

Wurst, M., Robles, A. Po, J., Luu, V-D, Brems, S., Marentije, M., Stoitsova, S., Quijada, L., Hoheisel, J., Stewart, M., Hartmann, C. and Clayton, C. (2009) An RNAi screen of the RRM-domain proteins of Trypanosoma brucei. Mol Biochem Parasitol, 163, 61-65.

Manful, T., Cristodero, M., Clayton, C. (2009) DRBD1 is the trypanosome homologue of the spliceosome-associated protein 49 in trypanosomes. Mol. Biochem. Parasitol. 166, 186-189

Bercovich, N., Clayton, C., Levin, MJ, Vazquez, MP. (2009) Identification of core components of the exon junction Colasante, C., Peña Diaz, P., Clayton, C and Voncken, F. (2009) Mitochondrial Carrier Family inventory of Trypanosoma brucei brucei: identification, expression and subcellular localisation Mol. Biochem. Parasitol. 167, 104-117

Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M and Clayton, C. (2009) The role of deadenylation in the degradation of unstable mRNAs in trypanosomes. Nucleic Acids Res 37, 5511-5528.  10.1093/nar/gkp571

Archer, S.K., Luu, V.-D., de Queiroz, R.A., Brems, S., Clayton, C. (2009) Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle. PLoS Pathogens 5, e1000565. doi:10.1371/journal.ppat.1000565

Queiroz, R., Benz, C., Fellenberg K., Hoheisel, J.D. and Clayton, C. (2009) Transcriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons. BMC Genomics 10, 495

Benz, C., Engstler, M., Hillmer, S. and Clayton, C. (2009) Depletion of 14-3-3 proteins in bloodstream-form Trypanosoma brucei inhibits VSG recycling. I. J. Parasitol. 40, 629-634.

Kramer, S., Queiroz, R., Ellis, L., Hoheisel, JD., Clayton, C and Carrington, M. (2010) The RNA helicase DHH1 is central to correct expression of many developmentally regulated mRNAs in trypanosomes. J. Cell Sci., 123, 699-711

Droll, D., Archer, S., Fenn, K., Delhi, P., Matthews, K and Clayton, C. (2010) The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation. FEBS Lett., 84, 1156-62

Manful, T. Mulindwa, J, Frank, FM, Clayton, CE,  and Matovu, E. (2010) A search for Trypanosoma brucei rhodesiense diagnostic antigens by proteomic screening and targeted cloning. PLoS One, 5, e9630.

Stewart, M., Haile, S., Jha, B.A., Cristodero, M., Li, C.H., and Clayton, C. (2010) Processing of a reporter mRNA in trypanosomes is not coupled to transcription by RNA polymerase II. Mol. Biochem. Parasitol. 172, 99-106

Haanstra, JR, Kerkhoven, EJ, van Tuijl, A, Blits, M,  Wurst, M, van Nuland, R, Albert, M-A, Michels, PAM, Bouwman, J, Clayton, C, Westerhoff, HV, Bakker, BM (2011) A domino effect in drug action: From metabolic assault towards parasite differentiation. Mol Microbiol. 79, 1365-2958

Benz, C., Mullindwa, J., Ouna, B. and Clayton, C. (2011) The Trypanosoma brucei zinc finger protein ZC3H18 is involved in differentiation. Mol. Biochem. Parasitol. 177, 148-51

Archer, SK., Inchaustegui, D., de Queiroz, R.A. and Clayton, C. (2011) The cell-cycle regulated transcriptome of an early-branching eukaryote. PloS One 6, e18425.

Manful, T, Fadda, A and Clayton, C. (2011) The role of the 5’-3’ exoribonuclease XRNA in transcriptome-wide mRNA degradation. RNA 17, 2039-2047. doi: 10.1261/rna.283731

Delhi, P., Queiroz, R., Carrington, M and Clayton, C. (2011) Is there classical nonsense-mediated mRNA decay in trypanosomes? PloS One 6, e25112.

Wurst, M, Selinger, B, Jha, BA, Klein, C, Queiroz, R and Clayton, C. (2012) Expression of the RNA Recognition Motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei. Mol Microbiol83, 1048-63

Ouna, BA, Stewart, M, Claudia Helbig, C and Clayton, C. (2012) The Trypanosoma brucei CCCH zinc finger proteins ZC3H12 and ZC3H13. Mol. Biochem. Parasitol. 183, 184-188

Ouna, BA, Nyambega, B, Manful, T, Helbig, C, Males, M, Fadda, A and Clayton, C. (2012) Depletion of trypanosome CTR9 leads to gene expression defects. PloS One 7, e34256

Färber, V, Erben, E, Sharma, S, Stoecklin, G and Clayton, C. (2013) Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs. Nucleic Acids Res 41, 1211-1222. doi: 10.1093/nar/gks1133

Colasante, C, Voncken, F, Manful, T, Ruppert, T, Tielens, AGM, van Hellemond, JJ, Clayton, C (2013) Proteins and lipids of glycosomal membranes from Leishmania tarentolae and Trypanosoma brucei. Faculty of 1000 Research, 2, 27.

Sanchez, L., Knudsen, G., ; Hartmann, C., De Muylder, G., Mascuch, S., Mackey, Z., Gerwick, L., Clayton, C., McKerrow, J., Linington, R. (2013) Examination of the Mode of Action of the Almiramide family of natural products against the Kinetoplastid parasite Trypanosoma brucei. J Nat Products, 76(4):630-41. doi: 10.1021/np300834q.

Droll, D., Minia, I., Fadda, A., Singh, A., Stewart, M., Queiroz, R., Clayton, C. (2013) Post-transcriptional regulation of the trypanosome heat shock response by a zinc finger protein. PloS Pathogens, 9, e1003286, doi:10.1371/journal.ppat.1003286.

Fadda, A., Färber, V., Droll, D. and Clayton, C. (2013) The roles of 3’-exoribonucleases and the exosome in trypanosome mRNA degradation. RNA 19, 937-947, doi: 10.1261/rna.038430.113

Lueong, S., Simo, G., Camara, M., Jamonneau, V., Kabore, J., Ilboudo, H., Bucheton, B., Hoheisel, J.D. and Clayton, C. The miRNA and mRNA signatures of peripheral blood cells in humans infected with Trypanosoma brucei gambiense. (2013) PloS ONE 8, e67312, doi: 10.1371/journal.pone.0067312.

Jha, B.A., Archer, S.K., and Clayton, C. (2013) The trypanosome pumilio domain protein PUF5. PloS ONE 8, e77371.

Klein, CA, Droll, D and Clayton, C. (2013) SUMOylation in Trypanosoma brucei. Peer J 1:e180

Loureiro I, Faria J, Clayton C, Ribeiro SM, Roy N, Santarém N, Cordeiro-da-Silva A (2013) Knockdown of asparagine Synthetase A Renders Trypanosoma brucei auxotrophic to asparagine. PLoS Negl Trop Dis 7: e2578. doi:10.1371/journal.pntd.0002578

Singh A, Minia I, Droll D, Fadda A, Clayton C, Erben E. (2014) Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks. Nucleic Acids Res 42, 4652-68; 10.1093/nar/gkt1416.

Nyambega, B, Helbig, C, Masiga, DK, Clayton, C, Levin, M. (2014) Proteins associated with SF3a60 in T. brucei. PloS ONE 9, e91956. doi: 10.1371/journal.pone.0091956

Mulindwa, J, Fadda, A, Merce, C, Matovu, E, Enyaru, J and Clayton, C. (2014) Methods to determine the transcriptomes of trypanosomes in mixtures with mammalian cells: the effects of parasite purification and selective cDNA amplification. PLoS Negl Trop Dis 8, e2806. doi:10.1371/journal.pntd.0002806.

Jha, BA, Fadda, A, Merce, C, Mugo, E, Droll, D and Clayton, C. (2014) Depletion of the trypanosome pumilio domain protein PUF2, or some other essential proteins, causes transcriptome changes related to coding region length Eukaryot. Cell 13, 664-74, doi: 10.1128/EC.00018-14

Erben, E, Fadda, A, Lueong, S, Hoheisel, JD and Clayton, C. (2014) Genome-wide discovery of post-transcriptional regulators in Trypanosoma brucei PLoS Pathogens 10, e1004178. doi:10.1371/journal.ppat.1004178

Begolo, D., Erben, E. and Clayton, C. (2014) Drug target identification using a trypanosome over-expression library. Antimicrob Agents Chemother. 58, 6260-4. doi: 10.1128/AAC.03338-14

Fadda, A, Ryten, M, Droll, D, Rojas, F, Färber, V, Haanstra, JR, Bakker, BM, Matthews, K and Clayton, C. (2014) Transcriptome-wide analysis of mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels. Mol Microbiol 94, 307-26. doi: 10.1111/mmi.12764

Loureiro, I, Faria, J, Clayton, C, Macedo-Ribeiro, S, Santarém, N, Roy, N, Cordeiro-da-Siva, A, Tavares, J. (2015) Ribose 5-phosphate isomerase B knockdown compromises Trypanosoma brucei bloodstream form infectivity.  PLoS Negl Trop Dis 9: e3430. doi: 10.1371/journal.pntd.0003430

Klein, C, Terrao, M, Inchaustegui Gil, D and Clayton, C (2015) Polysomes of Trypanosoma brucei: association with initiation factors and RNA-binding proteins. PloS ONE 10, e0135973, 10.1371/journal.pone.0135973.

Mulindwa, J, Mercé, C, Matovu, E, Enyaru, J, Clayton, C.  (2015) Transcriptomes of newly-isolated Trypanosoma brucei rhodesiense reveal hundreds of mRNAs that are co-regulated with stumpy-form markers. BMC Genomics 16, 1118.

Inchaustegui Gil, DP and Clayton, C.  (2016) Purification of messenger ribonucleoprotein particles via a tagged nascent polypeptide. PLoS ONE, 11, e0148131, 10.1371/journal.pone.0148131..

Minia, I, Clayton, C.  (2016) Regulating a post-transcriptional regulator: protein phosphorylation, degradation and translational blockage in control of the trypanosome stress-response RNA-binding protein ZC3H11. PLoS Pathogens 12, e1005514, 10.1371/journal.ppat.1005514

Antwi, E, Haanstra, J, Ramasamy, G, Jensen, B, Droll, D, Rojas, F, Minia, I, Terrao, M, Mercé, C, Matthews, K, Myler, PJ,, Parsons, M and Clayton, C. (2016) Integrative analysis of the Trypanosoma brucei gene expression cascade predicts differential regulation of mRNA processing and unusual control of ribosomal protein expression. BMC Genomics 17, 306, 10.1186/s12864-016-2624-3

Minia I, Mercé C, Terrao M, Clayton C. (2016) Translation regulation and RNA granule formation after heat shock of procyclic form Trypanosoma brucei: many heat-induced mRNAs are increased during differentiation to mammalian-infective forms.  PLoS Negl Trop Dis 10, e0004982

Klein C, Terrao M, Clayton C. (2017). The role of the zinc finger protein ZC3H32 in bloodstream-form Trypanosoma brucei. PLoS ONE 12, e0177901. 10.1371/journal.pone.0177901.

Chakraborty C, Fadda F, Erben E, Lueong, S, Hoheisel, J, Mugo E, Clayton C. (2017) Interactions of CAF1-NOT complex components from Trypanosoma brucei. Faculty of 1000 Research 6, 858. 10.12688/f1000research.11750.1

Mugo E, Egler F, Clayton C. (2017) Conversion of procyclic-form Trypanosoma brucei to the bloodstream form by transient expression of RBP10. Mol Biochem Parasitol 216C, 49-51. 10.1016/j.molbiopara.2017.06.009

Mugo E and Clayton C. (2017) Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei. PLoS Pathogens 13, e1006560

Mulindwa, J, Leiss, K, Ibberson, D, Kamanyi Marucha, K, Helbig, C, Nascimento, L, Silvester, E, Matthews, K, Matovu, E, Enyaru, J and Clayton, C (2018) Transcriptomes of Trypanosoma brucei rhodesiense from sleeping sickness patients, rodents and culture: effects of strain, growth conditions and RNA preparation methods. PLoS Negl Trop DisPLoS Negl Trop Dis 12, e0006280, 10.1371/journal.pntd.0006280.

Terrao M, Kimanyi Marucha K, Mugo E, Braun J, Droll D, Minia I, Egler F, and Clayton C. (2018) The suppressive cap-binding-complex factor 4EIP is required for normal differentiation. Nucleic Acids Res 46, 8993-9010.

Begolo D, Vincent IM, Giordani F, Pöhner I, Witty MJ, Rowan TG, Bengaly Z, Gillingwater K, Freund Y, Wade RC, Barrett MP, and Clayton C. (2018) The trypanocidal benzoxaborole AN7973 inhibits trypanosome mRNA processing. PLoS Pathogens 14, e1007315.



*Subjected to peer review

Clayton, C.E. (1979)  Immunsuppression in trypanosomasis and malaria, pp. 97-119 in "Role of the spleen in the immunology of parasitic diseases".  Tropical Diseases Research Series no 1, Schwabe and Co. A.G., Basel.

Rigby, P.W.J., Chia, W., Clayton, C.E., Lovett, M., Paucha, E. and Scott, M.S. (1980) The control of viral and cellular gene expression in Simian Virus 40 transformed cells.  Phil. Trans. Roy. Soc. Lond. B  210, 437-450.

Rigby, P.W.J., Chia, W., Clayton, C.E., Lewis, V., Lovett, M., Montano, X., Murphy, D. (1983) The integration of Simian Virus 40 into cellular DNA.  In "Biological Consequences of DNA structure and genome arrangement" 5th John Innes Symposium.  eds Chater, K. and Hopwood, D.A., Croom Helm, London.

Irving, D.O., Morry, M.J., Haas, L.O.C., Clayton, C.E., Wallach, M.G. and Cross, G.A.M. (1987) Characterization of a histidine rich protein-related gene from Plasmodium falciparum. pp 37-46 in: Molecular Strategies of Parasitic Invasion, eds N. Agabian, H. Goodman and N. Nogueira.  Alan R. Liss, New York. 

Clayton, C.E.  (1988)  Molecular Biology of the Trypanosomatidae. pp 2-56 in:  Genetic Engineering 7 ed. P.W.J. Rigby. Academic Press, London.

Cross, G.A.M., Bellofatto, V., Clayton, C.E. and Sherman, D.R. (1990) Using transfection to study gene expression in trypanosomes.  Biochem. Soc. Trans.18, 714-716.

Clayton, C.E., Fueri, J. and Mowatt, M.R. (1990) The procyclic surface protein of Trypanosoma brucei. In "Molecular aspects of Parasitism", ed. C.C. Wang, American Association for the Advancement of Science, Washington, DC. pp 55-63.

Clayton, C.E., (1992) Developmental regulation of nuclear gene expression in trypanosomes.  Pages 37-66 In: "Progress in Nucleic Acid Research and Molecular biology", vol 43, eds. W. Cohn and K. Moldave,  Academic Press, Florida.

Beverley, S.M. and Clayton, C.E., (1993) Transfection of Leishmania and Trypanosoma brucei by electroporation.  Chapter 25 in "Protocols in Molecular Parasitology" ed. J.E. Hyde,  Humana Press, New Jersey. pp 333-348.

Clayton, C.E. (1995) The Cell Biology of Disease. (Meeting review) Trends Microbiol. 3, 2-3

Clayton, C.E., Blattner, J. and Häusler, T. (1995) Protein trafficking in Kinetoplastida Microbiol.Rev.  59, 325-344.

Clayton, C.E. and Hotz, H.-R. (1996) Post-transcriptional control of PARP gene expression Mol. Biochem. Parasitol. 77, 1-6

Clayton, C.E. and Michels, P. (1996) Metabolic compartmentation in trypanosomes.  Parasitology Today 12, 465-471.

Hotz, H.-R., Biebinger, S., Flaspohler, J., and Clayton,C.E. (1998) PARP Gene expression: regulation at many levels. Francqui Symposium Proceedings and Mol. Biochem. Parasitol. 91, 131-143.

Clayton, C.E. et al.  (1998) Genetic nomenclature for Trypanosoma and Leishmania. Mol. Biochem. Parasitol. 97, 221-224 (Corresponding author, 42 authors)

Clayton, C.E. (1999) Genetic manipulation of Kinetoplastida. Parasitol. Today 15, 372-378.

Clayton, C.E., Maier, A., Lorenz, P., Blattner, J., Helfert, S., Krieger, S. (2000) Strange characteristics of kinetoplastid protists: targets for antiparasitic chemitherapy? Nova Acta Leopoldina 80, 15-25.

Clayton, C.E. (1999) Why do we need standard genetic nomenclature for parasites? Acta Tropica 75, 119-122
Roditi, I and Clayton, C.E. (1999) An unambiguous nomenclature for the major surface glycoprotein genes of procyclic forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 103, 99-100

Stuart, K., Panigrahi. A.K., Schnaufer, A., Drozdz, M., Clayton, C., and Salvati, R. (2002) Composition of the editing complex of Trypanosoma brucei. Phil. Trans. Roy. Soc. Lond. 357,

Clayton, C.E. (2002) Post-transcriptional regulation in parasites. In: Molecular Medical Parasitology Komuniecki, R.Nilsen, T., Marr, J., eds. Academic Press.

Clayton, C.E. (2002) Developmental regulation without transcriptional control? From fly to man and back again EMBO Journal 21, 1881-1888

Clayton, C.E., Estévez, A.M., Hartmann, C., Alibu, V.P., Field, M. and D. Horn. (2005). Down-regulating gene expression by RNA interference in Trypanosoma brucei. In G. Carmichael. (ed.), RNA interference. Humana Press.

Clayton, C.E. and Shapira, M. (2007) Post-transcriptional regulation of gene expression in Kinetoplastids Mol. Biochem. Parasitol. 156, 93-101

Archer, S., Queiroz, R., Stewart, M and Clayton, C. (2008) Trypanosomes as a model to investigate mRNA decay pathways. in "RNA turnover in Eukaryotes", Methods Enzymol., ed L. Maquat and M Kiledjian, Volume 448, , Chapter 18, pp359-377

Clayton, C., Schwede, A., Stewart, M., Robles, A., Benz, C., Po, J., Wurst, M., Queiroz, R. and Archer, S. (2008) Control of mRNA degradation in trypanosomes. Biochem. Soc. trans. 36, 520-1

Bakker, B.M., Krauth-Siegel, R.L., Clayton, C., Matthews, K., Girolami, M., Westerhoff, H.V., Michels, P.A.M., Breitling, R., Barrett, M. (2010) The Silicon trypanosome. Parasitology 137, 1333–1341

Clayton, C and Estevez, A (2010) pp 39-49 in: The exosomes of trypanosomes and other protists In “RNA Exosome”, ed Jensen, TH, Landes Bioscience.

Clayton, C (2010). Repetitive elements in parasitic protists. BMC Biol. 8, 64.

Clayton, C and Michaeli, S. (2011) 3’ processing in protists. Wiley Interdiscip Rev RNA. 2, 247-55.

Clayton, C. (2012) mRNA turnover in trypanosomes. In: RNA Metabolism in Trypanosomes, ed. A. Binderreif. Series “Nucleic Acids and Molecular Biology” (Series Editor: J. Bujnicki), Springer, Heidelberg.

Clayton, C. (2013) The regulation of trypanosome gene expression by RNA-binding proteins. PLoS Pathogens, 10s.1371/journal.ppat.1003680

Achcar, F, Fadda, A, Haanstra, JR, Kerkhoven, EJ, Kim, D-H, Leroux, AE, Papamarkou, T, Rojas, F, Bakker, BM, Barrett, MP, Clayton, C, Girolami, M, Krauth-Siegel, RL, Matthews, KR, Breitling, R. (2014) The Silicon Trypanosome: a test case of iterative model extension in systems biology. Adv Microb Physiol. 64:115-43. doi: 10.1016/B978-0-12-800143-1.00003-8

Erben, E, Chakraborty, C, Clayton, C. (2014) The CAF1-NOT complex of trypanosomes. Frontiers Genetics Research Topics 4, 299. doi: 10.3389/fgene.2013.00299

Clayton, C. (2014) Networks of gene expression control in trypanosomes. Mol Biochem Parasitol 195, 96-106.

Clayton, C. (2015) Trypanosomes. Textbook chapter.

Begolo, D. and Clayton, C. (2016) Discovery of the mechanism of action of compounds that target unicellular eukaryotic parasites. In: Müller S, Cerdan R, Radulescu O, editors. Comprehensive analysis of parasite biology,  from metabolism to drug discovery: Wiley. pp. 3-40.

Clayton, C. (2016) Gene expression in Kinetoplastids. Current Opinion in Microbiology, 32, 46-51.

Submitted or in preparation

Chakraborty, C and Clayton, C. Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30. (submitted)

Terrao, M, Kimanyi Marucha, K, Mugo, E, Braun, J, Droll, D, Minia, I, Egler, F, and Clayton, C. The suppressive cap-binding-complex factor 4EIP is required for normal differentiation of Trypanosoma brucei (in preparation)

Terrao, M, and Clayton, C. Post-translational mRNA control by PBP1-containing mRNA binding complexes. (in preparation)

Begolo, D, Vincent IM, Giordani, F, Witty MJ, Timothy G Rowan TG, Bengaly, Z, Gillingwater, K,  Freund, Y, Barrett MP, and Clayton, C. The oxaborole AN7973 inhibits trypanosome mRNA processing. (in preparation)



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