Publications by the Melchior Laboratory


Search Pubmed for publications by Frauke Melchior

Stankovic-Valentin, N., Kozaczkiewicz, L., Curth, K. and Melchior, F. (2009). An in vitro FRET-based assay for the analysis of SUMO conjugation and isopeptidase cleavage. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 241-251.

Werner, A., Moutty, M.C., Moeller, U. and Melchior, F. (2009). Performing in vitro sumoylation reactions using recombinant enzymes. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 187-199.

Meulmeester, E. and Melchior, F. (2008). Cell Biology: SUMO. Nature 452, 709-711.

Meulmeester, E., Kunze, M., Hsiaoh, H.H., Urlaub, H., Melchior, F. (2008). Mechanism and consequences of paralog specific sumoylation of USP25. Mol Cell 30, 610-619.

Hutten, S., Flotho, A., Melchior, F. and Kehlenbach, R.H. (2008). The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear import. Mol. Biol. Cell 10, 2300-2310.

Geiss-Friedlander, R. and Melchior F. (2008). SUMOylation. Chapter in: Encyclopedia of Molecular Pharmacology (Eds.: S. Offermann and W. Rosenthal), Springer Verlag, Part 19, 1163-1167.

Geiss-Friedlander, R. and Melchior F. (2007). Concepts in SUMOylation: a decade on. Nat. Rev. Mol. Cell Biol. 8, 947-956. 

Tirard, M., Almeida, O.F.X., Hutzler, P., Melchior, F. and Michaelidis, T.M. (2007). Sumoylation and proteasomal activity determine the transactivation properties of the mineralcorticoid receptor. Mol. Cell. Endocrin., 268, 20-29.

Bossis, G. and Melchior, F. (2006). Regulation of SUMOylation by reversible oxidation of SUMO conjugating enzymes. Mol. Cell 21, 349-357.

Bossis, G. and Melchior, F. (2006). SUMOylation. Chapter in: Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. (Ed.: K. Ruckpaul and D. Ganten), Springer.

Bossis, G. and Melchior, F. (2006). SUMO: regulating the regulator. Cell Div. 1:13.

Tiefenbach, J., Novac, N., Eck, M., Melchior, F. and Heinzel, T. (2006). SUMOylation of the co-repressor N-CoR modulates its capacity to repress transcription. Mol. Biol. Cell 17, 1643-1651.

Bossis, G., Chmielarska, K., Gaertner, U., Pichler, A., Stieger, E., and Melchior, F. (2005). A FRET-based assay to study SUMO modification in solution. Methods in Enzymology. 398, 20-32.

Palacios, S., Perez, L.H., Welsch, S., Schleich, S., Chmielarska, K., Melchior, F., and Krijnse Locke, J. (2005). Mol. Biol. Cell Quantitative SUMO-1 modification of a vaccinia virus protein is required for its specific localization and prevents its self-association. Mol Biol. Cell 16, 2822-2835.

Pichler, A, Knipscheer, P., Oberhofer, E., van Dijk, W.J., Koerner, R., Olsen, J.V., Jentsch, S., Melchior, F., and Sixma, T.K. (2005). SUMO modification of the ubiquitin conjugating enzyme E2-25K. Nat. Struct. & Mol. Biol.12, 264-269.

Beck, M., Foerster, F., Ecke, M., Plitzko, J., Melchior, F., Gerisch, G., Baumeister, W., Medalia, O. (2004). Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography. Science, 306, 1387-1390. 

Pichler, A., Knipscher, P., Saitoh, H., Sixma, T., and Melchior, F. (2004). SUMO E3 ligase RanBP2 is neither Hect- nor Ring-type. Nat. Struct. & Mol. Biol. 11, 984 - 991.

Pichler, A., and Melchior, F. (2004). SUMO E3 ligases. Chapter in: SUMOylation. Molecular Biology and Biochemistry (Ed.: Van Wilson, G.), Horizon Press.

Melchior, F. and Pichler, A (2004). SUMO Modification, in Encyclopedia of Biological Chemistry (W.J. Lennarz & M.D. Lane, eds), Elsevier, Oxford, Vol.4, pp. 130-134.

Liang, M., Melchior, F., Feng, X.H., and Lin, X. (2004). Regulation of Smad4 sumoylation and transforming growth factor-beta signaling by protein inhibitor of activated STAT1. J. Biol. Chem. 279, 22857-22865.

Swaminathan, S., Kiendl, F., Koerner, R., Lupetti, R., Hengst, L. and Melchior, F. (2004). RanGAP1*SUMO-1 is phosphorylated at the onset of mitosis and remains associated with RanBP2 upon NPC disassembly. J. Cell Biol. 164, 965-971.

Lin, X., Sun, B., Liang, M., Liang, YY., Gast, A., Hildebrand, J., Brunicardi, F.C., Melchior, F., and Feng, X.H. (2003). Opposed Regulation of Corepressor CtBP by SUMOylation and PDZ Binding. Mol. Cell 11, 1389-1396.

Lin, X., Liang, M., Liang, Y.Y., Brunicardi, F.C., Melchior, F., Feng, X.H. (2003). Activation of Transforming Growth Factor-b signaling by SUMO-1 modification of tumor suppressor Smad4/DPC4. J. Biol. Chem. 278, 18714-18719.

Melchior, F., Schergaut, M., and Pichler, A. (2003). SUMO: ligases, isopeptidases and nuclear pores. Trends Biochem Sci. 28, 612-618.

Melchior F., (2003). Ubiquitin-verwandte Proteine. BIOspektrum 6, 691-693.

Connor, M.K., Kotchetkov, R., Cariou, S., Beniston, R.G., Resch, A., Lupetti, R., Melchior, F., Hengst, L., and Slingerland, J.M. (2003). CRM1/Ran-mediated nuclear export of p27Kip1 involves a nuclear export signal and links p27 export and proteolysis. Mol. Biol. Cell. 14, 201-213.

Sapetschnig, A., Rischitor, G., Braun, H., Doll, A., Schergaut, M., Melchior, F. and Suske, G. (2002). Transcription factor Sp3 is silenced through SUMO modification by PIAS1. EMBO J. 21, 5206-5215.

Kirsh, O., Seeler, J.S., Pichler, A., Gast, A, Mueller, S., Miska, E., Mathieu, M., Harel-Bellan, A., Kouzarides, T., Melchior, F. and Dejean, A. (2002). The SUMO E3 Ligase RanBP2 Promotes Modification of the HDAC4 Deacetylase. EMBO J. 21, 2682-2691.

Pichler, A. and Melchior, F. (2002). Ubiquitin-related modifier SUMO1 and nucleocytoplasmic transport. Traffic 3, 381-387.

Pichler, A., Gast, A., Seeler, J.S., Dejean, A. and Melchior, F. (2002). The nucleoporin RanBP2 is a SUMO1 E3 Ligase. Cell 108, 109-120.

Swaminathan, S. and Melchior, F. (2002). Calcium, viruses and nuclear pores: Meeting report from the EMBO workshop "Signal-transduction-mediated regulation of nuclear transport", Strasbourg, France, 11-14 August 2001. Traffic 3, 74-76.

Melchior, F. (2002). A decade's worth of Ran. Trends Cell Biol. 12, 203.

Melchior, F. and Hengst, L. (2002). SUMO-1 and p53, Cell Cycle 1(4), 1, 245-249.

Swaminathan, S. and Melchior, F. (2002). Nucleocytoplasmic transport: more than the usual suspects. Developmental Cell 3, 304-306.

Melchior, F. (2001). Ran GTPase cycle: One mechanism - two functions. Curr Biol., 11, R257-60.

Sachdev, S., Bruhn, L., Sieber, H., Pichler, A., Melchior, F. and Grossschedl, R. (2001). PIASy represses LEF1 activity by stimulating SUMO conjugation and targeting of LEF1 to nuclear bodies. Genes Dev., 15, 3088-103.

Melchior, F. (2000). SUMO-1 - Non-Classical Ubiquitin. Annu. Rev. Cell Dev. Biol.,16, 591-626.

Melchior, F. and Hengst, L. (2000). Mdm2-SUMO1: Is bigger better? Nature Cell Biol., 2, E161-E163.

Gast, A., Pichler, A., Swaminathan, S., and Melchior, F. (2000). SUMO shifts to new functions. The ELSO Gazette 1 (http://www.the-elso-gazette/magazines/issue1).

Bayer, P., Andreas Arndt, A., Metzger, S., Mahajan, R., Melchior, F., Jaenicke, R., and Becker, J. (1998). Structure determination of the small ubiquitin-related modifier SUMO-1. J. Mol. Biol. 280, 275-286.

Melchior, F. and Gerace, L. (1998). Two-way trafficking with Ran? Trends Cell Biol. 8, 175-179.

Lee, G.W., Melchior, F., Matunis, M.J., Mahajan, R., Tian, Q., and Anderson, P. (1998). Modification of Ran GTPase-activating protein by the small ubiquitin-related modifier SUMO-1 requires Ubc9, an E2-type ubiquitin-conjugating enzyme homologue. J. Biol. Chem., 273, 6503-6507.

Mahajan, R., Gerace, L. and Melchior, F. (1998). The C-terminal domain of RanGAP1 contains the acceptor site for SUMO1 modification and is sufficient for targeting to the nuclear envelope. J. Cell Biol. 140, 259-270.

Melchior, F. (1998). Nuclear protein import in a permeabilized cell assay. in: Protein targeting protocols (Editor R. A. Clegg) Methods in Molecular Biology 88, 265-273.

Delphin, C., Guan, T., Melchior, F. and Gerace, L. (1997). RanGTP targets p97 to RanBP2 at the cytoplasmic periphery of the nuclear pore complex. Mol. Biol. Cell 8, 2379-2390.

Preisig-Mueller, R., Gehlert, R., Melchior, F., Stietz, U., and Kindl, H. (1997). Plant Polyketide Synthases leading to Stilbenoids have a domain catalyzing malonyl-CoA:CO2 exchange, malonyl-CoA decarboxylation, and covalent enzyme modification and a site for chain lengthening. Biochemistry 36, 8349-8358.

Mahajan, R., Delphin, C., Guan, T., Gerace, L. and Melchior, F. (1997). A small ubiquitin related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107.

Melchior, F., and Gerace, L. (1995). Mechanisms of nuclear protein import. Curr. Opin. Cell Biol. 7, 310-318.

Melchior, F., Guan, T., Yokoyama, N., Nishimoto, T., and Gerace, L. (1995). GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol. 131, 1-11.

Melchior, F., Sweet, D. J., and Gerace, L. (1995). Analysis of Ran/TC4 function in nuclear protein import. Methods Enzym. 257, 279-291.

Melchior, F., Guan, T., Paschal, B., and Gerace, L. (1995). Biochemical and structural analysis of nuclear protein import. Cold Spring Harbor Sym. Quant. Biol. LX, 707-716.

Becker, J., Melchior, F., Gerke, V., Bischoff, F. R., Ponstingl, H., and Wittinghofer, A. (1995). RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J. Biol. Chem. 270, 11860-11865.

Melchior, F., Paschal, B., Evans, J., and Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659.

Melchior, F., Weber, K., and Gerke, V. (1993). A functional homologue of the RNA1 gene product in Schizosaccharomyces pombe: Purification, biochemical characterization, and identification of a Leucine-rich Repeat motif. Mol. Biol. Cell. 4, 569-581.

Doherty, N. M., Hogarth, G., Knox, S. A. R., Macpherson, K. A., Melchior, F., Morton, D. A. V. and Orpen, A. G. (1992). Phosphorus-Carbon bond cleavage at a di-iron centre: synthesis of m-phosphidomethyl complexes [Fe2(CO)6(m-CH2PR2)(m-PR2)] from [Fe2(CO)6(m-CO)(m-R2PCH2PR2)]. Inorganica Chimica Acta, 198-200, 257-270.

Melchior, F., and Kindl, H. (1991). Coordinate and elicitor-dependent expression of Stilbene Synthase and Phenylalanine Ammonia-Lyase genes in Vitis cv. Optima. Arch. Biochem. Biophys. 288, 552-557.

Liswidowati, Melchior, F., Hohmann, F., Schwer, B., and Kindl, H. (1991). Induction of Stilbene Synthase by Botrytis cinerea in cultured Grapevine cells.  Planta 183, 307-314.

Melchior, F., and Kindl, H. (1990). Grapevine Stilbene Synthase cDNA only slightly differing from Chalcone Synthase cDNA is expressed in Escherichia coli into a catalytically active Enzyme. FEBS Lett. 268, 17-20.

Doherty, N. M., Hogarth, G., Knox, S. A. R., Macpherson, K. A., Melchior, F., and Orpen, A. G. (1986). Phosphorus-Carbon bond cleavage at a di-iron centre. Conversion of m-R2PCH2PR2 to m-R2PCH2 and m-PR2: Crystal structures of [Fe2(CO)4(m-Ph2PCH2)(m-PPH2)(m-Me2PCH2PMe2)] and [Fe(CO)6(m-PhPCH(Me)P(Ph)(C6H4-o))]. J. Chem. Soc., Chem. Commun., 540-542.