Ruprecht-Karls-Universität Heidelberg

Stefan Pfeffer
ZMBH Junior Research Group Leader

ZMBH
Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Tel. +49 (0) 6221 - 54 6849
Fax +49 (0) 6221 - 54 5891
s.pfeffer@zmbh.uni-heidelberg.de

old: pfeffer@biochem.mpg.de




Welcome to the Pfeffer lab!

(for further information, see MPI for Biochemistry in Martinsried)

 



Cryo-EM analysis of the co-translational machinery for protein folding and maturation


Co-translational folding and maturation of proteins require an intricate network of folding chaperones and processing enzymes that act on the growing nascent protein in a co-translational manner and co-purify with polysomal complexes. Structural information on ribosome-nascent chain-chaperone complexes is sparse, because the involved interactions are mostly transient, labile and possibly highly flexible for chaperones binding exclusively to the growing nascent protein. This renders the involved assemblies inaccessible to classical reductionist structural biology approaches that rely on extensive biochemical purification and require conformationally homogenous particle populations for averaging.

We consequently pursue a different approach and image these processes using cryo electron tomography (cryo-ET)-based strategies, which can reveal the three-dimensional arrangement of individual macromolecules even in crowded native microenvironments at molecular resolution and therefore render extensive biochemical purification unnecessary. This approach allows us to analyze the three-dimensional spatial distribution of ribosomes, chaperones and processing enzymes for individual native polysomal assemblies under conditions that preserve the labile and transient interactions governing co-translational protein folding and maturation. We study defined polysomal assemblies engaged in the synthesis of model substrates in various organisms, visualizing these assemblies in both a non-cellular context and in sections of vitrified unperturbed cells obtained using focused ion beam (FIB) milling.

Structural analysis of polysomal assemblies in the native cellular context. (A) Slice through a representative cryo electron tomogram of an unperturbed vitrified yeast cell thinned to electron-transparent thickness using Focused Ion Beam (FIB) milling. The rough endoplasmic reticulum (rER), the Golgi apparatus (G) and several example ribosomes (arrow heads) are indicated. (B) Three-dimensional visualization of the same tomogram with cellular membranes (grey) and detected ribosomes (large subunit: cyan, small subunit: yellow) shown. (C) Example polysome within the tomogram with consecutive ribosomes numbered and depicted in alternating color. The trace of the messenger RNA (mRNA) molecule was inferred based on the positions of mRNA entry (green) and exit (red) sites on the small ribosomal subunitsext.


In order to gain the most detailed structural insights possible into co-translational protein folding and maturation, we also innovate cryo-ET based data acquisition strategies and image processing schemes, optimizing them for high resolution. In particular, we integrate complementary information from different cryo-EM approaches to gain information on the three-dimensional architecture of the specimen while retaining high-resolution signal for structure determination.

 

 

Selected publications

Original Research Papers

Pfeffer, S., Dudek, J., Schaffer, M., Ng, B., Albert, S., Plitzko, J.M., Baumeister, W., Zimmermann, R., Freeze, H.H., Engel, B.D., Förster, F., 2017. Dissecting the molecular organization of the translocon-associated protein complex. Nat Commun 8, 14516.

Pfeffer, S., Burbaum, L., Unverdorben, P., Pech, M., Chen, Y., Zimmermann, R., Beckmann, R., Förster, F., 2015. Structure of the native Sec61 protein-conducting channel. Nat Commun 6, 8403.

Pfeffer, S.*, Woellhaf, M.W.*, Herrmann, J.M., Förster, F., 2015. Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography. Nat Commun 6, 6019.

Pfeffer, S.*, Dudek, J.*, Gogala, M., Schorr, S., Linxweiler, J., Lang, S., Becker, T., Beckmann, R., Zimmermann, R., Förster, F., 2014. Structure of the mammalian oligosaccharyl-transferase complex in the native ER protein translocon. Nat Commun 5, 3072.

Pfeffer, S., Brandt, F., Hrabe, T., Lang, S., Eibauer, M., Zimmermann, R., Förster, F., 2012. Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes. Structure 20, 1508-1518.

Englmeier, R., Pfeffer, S., and Förster, F., 2017. Structure of the Human Mitochondrial Ribosome Studied In Situ by Cryoelectron Tomography. Structure 25, 1574-1581.

Khoshouei, M.*, Pfeffer, S.*, Baumeister, W., Förster, F., Danev, R., 2016. Subtomogram analysis using the Volta phase plate. J. Struct. Biol. 197, 94-101.

Mahamid, J., Pfeffer, S., Schaffer, M., Villa, E., Danev, R., Cuellar, L.K., Förster, F., Hyman, A.A., Plitzko, J.M., Baumeister, W., 2016. Visualizing the molecular sociology at the HeLa cell nuclear periphery. Science 351, 969-972.

Chen, Y., Pfeffer, S., Hrabe, T., Schuller, J.M., Förster, F., 2013. Fast and Accurate Reference-Free Alignment of Subtomograms. J. Struct. Biol. 182, 235-245.

Chen, Y., Pfeffer, S., Fernandez, J.J., Sorzano, C.O., Förster, F., 2014. Autofocused 3D classification of cryoelectron subtomograms. Structure 22, 1528-1537.

Hrabe, T., Chen, Y., Pfeffer, S., Cuellar, L.K., Mangold, A.V., Förster, F., 2012. PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis. J. Struct. Biol. 178, 177-188.


Review Articles

Pfeffer, S., Dudek, J., Zimmermann, R., Förster, F., 2016. Organization of the native ribosome-translocon complex at the mammalian endoplasmic reticulum membrane. Biochim Biophys Acta 10, 2122-2129.

Pfeffer, S., Förster, F., 2016. Sec61: A static framework for membrane-protein insertion. Channels (Austin) 10, 167-169.

Dudek, J., Pfeffer, S., Lee, P.H., Jung, M., Cavalie, A., Helms, V., Förster, F., Zimmermann, R., 2014. Protein Transport into the Human Endoplasmic Reticulum. J. Mol. Biol. 427, 1159-1175.

Lang, S., Pfeffer, S., Lee, P.H., Cavalié, A., Helms, V., Förster, F., Zimmermann, R., 2017. An Update on Sec61 Channel Functions, Mechanisms, and Related Diseases. Front Physiol. 8, 887.



* = equal contribution