Ruprecht-Karls-Universitšt Heidelberg

Elmar Schiebel
Group Leader in the
DKFZ-ZMBH Alliance

Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Tel.: + 49-6221 54 6814
Fax: +49-6221 54 5892

Segregation of chromosomes in mitosis and regulation of mitotic exit

During mitosis and meiosis, the duplicated genetic information, the chromosomes, are separated by a precise machine, which is called the mitotic or meiotic spindle, to opposite spindle poles. After the subsequent cell division, each daughter cell has an identical chromosome set. Defects in this machine can lead to cancer or Down-Syndrom. Our work aims to understand how this machine works and is regulated. For this, we purify the protein components of the mitotic spindle and analyze their properties in the test tube. Using the new tools of genome editing (CRISPR-Cas9), we manipulate genes that code for mitotic spindle components in human tissue culture cells and monitor the defects by microscopy. In collaboration with chemists and structural biologists, we develop drugs that impair this machine specifically in dividing cancer cells.


Selected publications

Rüthnick, D., A. Neuner, F. Dietrich, D. Kirrmaier, U. Engel, M. Knop, and E. Schiebel. (2017). Characterization of spindle pole duplication reveals a regulatory role for NPCs. J. Cell Biol., accepted.

Lin, T., A. Neuner, D. Flemming, P. Liu, T. Chinen, U. Jaekle, R. Arkowitz, and E. Schiebel. (2016). MOZART1 and gamma-tubulin complex receptors are both required to turn gamma-TuSC into an active microtubule nucleation template. J. Cell Biol., Dec. 5. pii:jcb.201606092 (see Abstract).

Cerikan, B., R. Shaheen, G.P. Colo, C. Gläßer, S. Hata, K.-P. Knobeloch, F.S. Alkuraya, R. Fässler, and E. Schiebel. (2016). Cell intrinsic adaptation arising from chronic ablation of a key Rho GTPase regulator. Dev. Cell , 39:28-43 (see Abstract). Interview with B. Cerikan

Chen, N.-P., B. Uddin, R. Voit, and E. Schiebel. (2016). Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion. Proc. Natl. Acad. Sci. USA, 113:990-995 (see Abstract).

Seybold, C., M. Elserafy, D. Rüthnick, M. Ozboyaci, A. Neuner, B. Flottmann, M. Heilemann, R.C. Wade, and E. Schiebel. (2015). Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope. J. Cell Biol., 209:843-861. (see Abstract).

Lin, T., A. Neuner, Y. Schlosser, A. Scharf, L. Weber, and E. Schiebel. (2014). Cell-cycle dependent phosphorylation of yeast pericentrin regulates gamma-TuSC-mediated microtubule nucleation. eLife, 3:e02208 (see Abstract).

Gombos, L., A. Neuner, M. Berynskyy, L.L. Fava, R.C. Wade, C. Sachse, and E. Schiebel. (2013). GTP regulates the microtubule nucleation activity of gamma-tubulin. Nature Cell Biol., 15:1317-1327 (see Abstract).

Erlemann, S., A. Neuner, L. Gombos, R. Gibeaux, C. Antony, and E. Schiebel. (2012). An extended gamma-tubulin ring functions as a stable platform in microtubule nucleation. J. Cell Biol., 197:59-74 (see Abstract).

Roostalu, J., C. Hentrich, P. Bieling, I.A. Telley, E. Schiebel* and T. Surrey*. (2011). Directional switching of the kinesin Cin8 through motor coupling. Science, 332:94-99 (see Abstract). * Co-corresponding authors.

Mardin, B.R., C. Lange, J.E. Baxter, T. Hardy, S.R. Scholz, A.M. Fry and E. Schiebel. (2010). Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction. Nature Cell Biol., 12:1166-1176 (see Abstract).

Khmelinskii, A., P.J. Keller, H. Lorenz, E. Schiebel* and M. Knop*. (2010). Segregation of yeast nuclear pores. Nature, 466(7305):E1 (see Abstract). * Co-corresponding authors.

Khmelinskii, A., J. Roostalu, H. Roque, C. Antony and E. Schiebel. (2009). Phosphorylation-dependent protein interactions at the spindle midzone mediate cell cycle regulation of spindle elongation. Dev. Cell, 17:244-256 (see Abstract).

Khmelinskii, A. , C. Lawrence, J. Roostalu and E. Schiebel. (2007). Cdc14-regulated midzone assembly controls anaphase B. J. Cell Biol., 177: 981-993 (see Abstract).

Pereira, G.* and E. Schiebel*. (2005). Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol. Cell, 19: 209-221 (see Abstract). * Co-corresponding authors.

Pereira, G.* and E. Schiebel*. (2003). Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science, 302: 2120-2124 (see Abstract). * Co-corresponding authors.

Tanaka, T.U., N. Rachidi, C. Janke, G. Pereira, M. Galova, E. Schiebel, M.J.R. Stark and K. Nasmyth. (2002). Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. Cell, 108: 317-329. (see Abstract).

Pereira, G., T.U. Tanaka, K. Nasmyth and E. Schiebel (2001). Modes of spindle pole body inheritance and segregation of the Bfa1p/Bub2p checkpoint protein complex. EMBO J., 20: 6359-6370 (see Abstract).

Pereira, G., T. Höfken, J. Grindlay, C. Manson and E. Schiebel. (2000). The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol. Cell, 6: 1-10 (see Abstract).

underlined: PhD students or postdocs of the Schiebel lab