Protocols
Protein staining
Colloidal Coomassie (EMBL)
Blue silver (Candiano et al.)
Silver staining (EMBL)Silver staining should be used only, if colloidal coomassie is not sensitive enough for picking the protein spot/band. Silver stained gel plugs must be destaind before storage to avoid loss of peptides. (Richert S et al.)
Sample preparation
In-Gel Digestion (ZMBH)
MALDI TOF MS (ZMBH)
NanoESI QTOF MS (ZMBH)
Contact
Dr. Thomas Ruppert
ZMBH
Im Neueneimer Feld 282
D-69120 Heidelberg
Phone: +49-6221-54-6895
Fax: +49-6221-54-5891
t.ruppert@zmbh.uni-heidelberg.de
Biomolecular Chemistry /
Mass Spectrometry
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