Protocols












Protein staining

Colloidal Coomassie (EMBL)
Blue silver (Candiano et al.)
Silver staining (EMBL)

Silver staining should be used only, if colloidal coomassie is not sensitive enough for picking the protein spot/band. Silver stained gel plugs must be destaind before storage to avoid loss of peptides. (Richert S et al.)

Sample preparation

In-Gel Digestion (ZMBH)
MALDI TOF MS (ZMBH)
NanoESI QTOF MS (ZMBH)












Contact

Dr. Thomas Ruppert
ZMBH
Im Neueneimer Feld 282
D-69120 Heidelberg
Phone: +49-6221-54-6895
Fax: +49-6221-54-5891
t.ruppert@zmbh.uni-heidelberg.de






Biomolecular Chemistry /
Mass Spectrometry


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