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CFMP
Core Facility for
Mass
Spectrometry & Proteomics |
The Core Facility for Mass Spectrometry and Proteomics is an infrastructure facility of the Center for Molecular Biology
of Heidelberg University (ZMBH). We support the research scientists of the FoF1 / Heidelberg and Mannheim Life Sciences
campus with high-performance mass spectrometry aimed at protein analytics and proteomics.
Regular services:
- Molecular weight determination of intact proteins (protocol)
- Protein identification of gel separated proteins (protocol)
- Identification of interaction partners from Co-IP experiments (protocol)
- Analysis of proximity labeling (BioID) experiments (protocol)
- Proteome analysis (protocol)
Additional services (detailed discussion before is a prerequisite):
- Analysis of post- translational modifications (e.g. phosphorylation, succinylation)
- Identification of cross- linked peptides of purified proteins and protein complexes
- Cell- wide or organelle- wide interactome analysis (co- fractionation MS)
- Please, contact us for additional application that may fit your needs.
Sample submission:
- Please, familiarize yourself with the recommendations on sample preparation appropriate for the chosen service and sample submission guideline.
Before submitting a request, please schedule an appointment with Marcin Luzarowski.
m.luzarowski@zmbh.uni-heidelberg.de
- Details about sample submission can be found here:
Important notes:
- Each facility user is obliged to be familar and sign CFMP user regulations.
- The contribution of the CFMP to the research projects should be appropriately acknowledged.
It helps CFMP to be recogmized and ease applications for external funding
and the acquisition of new instruments. As scientific contribution can be considered:
- Development of new analytical method
- Help with experimental design
- Generation and/or evaluation of the data
- Manuscript preparation, generation of figures, supplementary data preparation
and raw data submission
- Samples are processed on a first come first serve basis. Sample processing includes protein
digestion into peptides, sample clean-up, peptide seperation and quantification using LC-MS
and initial database search. Regular services are processed usually within 2-4 weeks.
Additional services usally take up to 8 weeks.
- Detailed price list including project examples can be found here
Please, note that the final price has to be discussed with the head of the CFMP.
Acknowledgment:
We extend our gratitude to our colleagues at EMBL for engaging discussions on website organization
and generously sharing informative content from their website.
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