The ZMBH Core Facility for Biomolecular Chemistry provides timely state-of-the-art services for identification and characterization of proteins to support the research of the ZMBH investigators. Capacity permitting, samples from outside the ZMBH can be analyzed at a highly competitive prize.
Services offered:
- Protein identification by MALDI-TOF/TOF mass spectrometry
- Peptide sequencing by nanoESI QTOF mass spectrometry
- Determination of the exact molecular weight of proteins
- Multidimensional protein identification technology (MudPIT)
In addition the Core Facility is involved in the development of new methods for analyzing proteins.
- Identification of posttranslational modifications
- Determination of the N-terminus and C-terminus of proteins
- Analysis of protein conformation by amide hydrogen-1H/2H-exchange
Instrumentation:
The facility is equipped with modern, high-end instruments for protein and peptide analytics.
SAMPLE PREPARATION
- Pipetting robot: Digest Pro (Intavis)
- nanoHPLC: ULTIMATE (LC-Packings/Dionex)
- equipped with FAMOS and SWITCHOS
MASS SPECTROMETERS
- Maldi-Tof MS: ULTRAFLEX TOF/TOF (Bruker)
- ESI-Qtof MS: QSTAR PULSAR (Applied Biosystems)
- ESI_Ion Trap MS: LCQ classic (Thermofinnigan)
-
Back to Biomolecular Chemistry
Download Order Form
