Ruprecht-Karls-Universitšt Heidelberg
Protocols / Plasmids

Methods

Ritterhoff T, Das H, Hao Y, Sakin V, Flotho A, Werner A, Melchior F (2016). Reconstitution of the Recombinant RanBP2 SUMO E3 Ligase Complex. Methods Mol Biol 2016; 1475:41-54

Barysch, S.V., Dittner, C., Flotho, A., Becker, J., Melchior, F. (2014). Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies.Nat. Protoc. 2014 Apr;9(4):896-909.

Stankovic-Valentin, N., Kozaczkiewicz, L., Curth, K. and Melchior, F. (2009). An in vitro FRET-based assay for the analysis of SUMO conjugation and isopeptidase cleavage. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 241-251.


Werner, A., Moutty, M.C., Moeller, U. and Melchior, F. (2009). Performing in vitro sumoylation reactions using recombinant enzymes. Methods in Molecular Biology: SUMO Protocols (Editor H. D. Ulrich): 497: 187-199.


Bossis, G., Chmielarska, K., Gaertner, U., Pichler, A., Stieger, E., and Melchior, F. (2005). A FRET-based assay to study SUMO modification in solution. Methods in Enzymology. 398, 20-32.

 


 

Our monoclonal anti SUMO1 (aa76-86) antibody can be obtained via the non-profit Hybridoma Bank DSHB: "http://dshb.biology.uiowa.edu/SUMO1-76-86"









































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