Dirk Görlich
Nucleo-cytoplasmic transport
Current research
The nuclear envelope divides eukaryotic cells into distinct
nuclear and cytoplasmic compartments. mRNA, tRNA and ribosomes
are made in the nucleus and need to be exported to the cytoplasm
where they function in translation. Conversely, all nuclear proteins
are synthesised in the cytoplasm and need to be imported into
the nucleus. This nucleocytoplasmic transport proceeds through
the nuclear pore complexes (NPCs) and is largely mediated by
specific carriers such as importins or exportins. The loading
and unloading of these carriers with cargo is co-ordinated by
the RanGTPase system.
Projects for a doctoral thesis
NPCs are giant molecular machines that function as highly
selective gates between nucleus and cytoplasm. They allow a rapid
passage of some molecules, such as nuclear transport receptors,
but represent a firm permeability barrier for others. The molecular
basis for this selectivity as well as the molecular mechanism(s)
of NPC function are major, unresolved issues in the nuclear transport
field. These questions are the current focus of our laboratory
and will be addressed by microscopical techniques, by biochemical,
biophysical, molecular biological approaches, as well as by computer
simulations.
Contact:
Dirk Görlich
ZMBH
Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany
Tel.: +49-6221-54 58 84
Fax: +49-6221-54 58 93
email: dg@zmbh.uni-heidelberg.de
http://www.zmbh.uni-heidelberg.de/Goerlich/default.html
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Selected publications
Görlich, D. Kutay, U. (1999) Transport between the cell
nucleus and the cytoplasm. Annu. Rev. Cell Dev. Biology, 15,
607-660 (Review).
Kutay, U., Bischoff, F.R., Kostka, S., Kraft, R., and Görlich,
D. (1997). Export of importin a from the nucleus is mediated
by a specific nuclear transport factor. Cell 90, 1061-1071.
Ribbeck, K., Lipowsky, G., Kent, H.M., Stewart, M. and Görlich,
D.. (1998). NTF2 mediates nuclear import of Ran. EMBO J. 17,
6587-6598.
Jäkel, S., Albig, W., Schwamborn, K., Doenecke, D., and
Görlich, D. (1999). The importinb/ importin7 heterodimer
is a functional nuclear import receptor for histone H1. EMBO
J. 18, 2411-2423.
Lipowsky, G., Bischoff, F.R., Schwarzmaier, P., Kraft, R., Hartmann,
E., Kutay, U., and Görlich, D. (2000). Exportin 4: a mediator
of a novel nuclear export pathway in higher eukaryotes. EMBO
J., 19, 4362-4371
.

Figure shows fluorescent importin beta bound to nuclear
pore complexes.
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