Publications by the Knop Lab
search pubmed for current publications by M. Knop
YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries.
Monitoring Protein Dynamics in Protein O-Mannosyltransferase Mutants In Vivo by Tandem Fluorescent Protein Timers.
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis.
Genome-wide C-SWAT library for high-throughput yeast genome tagging.
Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins .
Mapping Degradation Signals and Pathways in a Eukaryotic N-terminome.
Cells Escape an Operational Mitotic Checkpoint through a Stochastic Process .
Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities
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Characterization of spindle pole body duplication reveals a regulatory role for
nuclear pore complexes .
Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis
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Temporal and compartment-specific signals coordinate mitotic exit with spindle position. .
The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use.
πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes.
Protein Abundance Control by Non-coding Antisense Transcription.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy .
Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers .
Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress .
Live-cell multiphoton fluorescence correlation spectroskopy with an improved large Stokes shift fluorescent protein .Protein quality control at the inner nuclear membrane .PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast .A memory system of negative polarity cues prevents replicative aging .Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis .Analytical model for macromolecular partitioning during yeast cell division PSFj: know your fluorescence microscope .Analysis of protein dynamics with tandem fluorescent protein timers .Tracking protein turnover and degradation by microscopy: photo-switchable versus time-encoded fluorescent proteins .Determinants of robustness in spindle assembly checkpoint signalling Directional tissue migration through a self-generated chemokine gradient .Donà E, Barry JD, Valentin G, Quirin C, Khmelinskii A, Kunze A, Durdu S, Newton LR, Fernandez-Minan A, Huber W, Knop M, Gilmour D.When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved .Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy .Tandem fluorescent protein timers for in vivo analysis of protein dynamics .TIPI: TEV protease-mediated induction of protein instability . Seamless gene tagging by endonuclease-driven homologous recombination . Khmelinskii A, Meurer M, Duishoev N, Delhomme N, Knop M.Exploiting spore-autonomous fluorescent protein expression to quantify meiotic chromosome behaviors in Saccharomyces cerevisiae .Yeast cell morphology and sexual reproduction--a short overview and some considerations .Quantitative fluorescence imaging of protein diffusion and interaction in living cells . Genomic promoter replacement cassettes to alter gene expression in the yeast Saccharomyces cerevisiae .Artificial tethering to nuclear pores promotes partitioning of extrachromosomal DNA during yeast asymmetric cell division .Segregation of yeast nuclear pores .Evolution of mutational robustness in the yeast genome: a link to essential genes and meiotic recombination hotspots .Efficient protein depletion by genetically controlled deprotection of a dormant N-degron .The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis .Reconstitution of Mdm2-dependent post-translational modifications of p53 in yeast .Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling .Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane .Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast .Evolution of the hemiascomycete yeasts: on life styles and the importance of inbreeding .Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis .System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae .Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system .Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization .Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis .Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast .A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M. Yeast. 2004 Aug;21(11):947-62.Regulation of exocytotic events by centrosome-analogous structures . Taxis, C. & Knop, M. Topics Curr. Gen. 2004 10 193-207Prospore membrane formation: how budding yeast gets shaped in meiosis .Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions .mproved version of the red fluorescent protein (drFP583/DsRed/RFP) .Prospore membrane formation linked to the leading edge protein (LEP) coat assembly .A screen for genes required for meiosis and spore formation based on whole-genome expression .Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis .Microtubule organization by the budding yeast spindle pole body .Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy .Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines .Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication .Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation .Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body .Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p .The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication .N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast .The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment .Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast .Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast .Vacuolar/lysosomal proteolysis: proteases, substrates, mechanisms .
