Ruprecht-Karls-Universität Heidelberg

Contact us:
Zentrum fr Molekulare Biologie der Universitt Heidelberg (ZMBH)
Im Neuenheimer Feld 282
Room 401
69120 Heidelberg, Germany
Tel.: +49-6221-54 6813
Fax: +49-6221-54 5894
ms-service@zmbh.uni-heidelberg.de


Equipment

Mass spectrometers

Q-Exactive HF MS (Thermo) coupled with Dionex Ultimate 3000 RSLCnano (Thermo)
The Q-Exactive is a high performance hybrid mass spectrometer combining the highfield Orbitrap analyzer with a fast scanning quadrupol analyzer. It is coupled with a Dionex Ultimate 3000 RSLCnano UPLC system (Thermo) for exceptional peptide separation. The Q-Exactive combines the very high resolution of the highfield Orbitrap analyzer with the fast scan speed and the precise mass filtering capabilities of a quadrupole.

Orbitrap Elite MS (Thermo) coupled with Dionex Ultimate 3000 RSLCnano (Thermo)
The Orbitrap Elite is a high performance hybrid mass spectrometer combining the highfield Orbitrap analyzer with a dual pressure linear ion trap. It is coupled with a Dionex Ultimate 3000 RSLCnano UPLC system (Thermo) allowing exceptional peptide separation. The Orbitrap Elite offers multiple fragmentation techniques (CID, HCD and ETD).

LTQ Orbitrap XL MS (Thermo) coupled with nanoUPLC Aquity (Waters)
The Orbitrap is a high performance hybrid mass spectrometer combining the Orbitrap analyzer with the linear ion trap. It is coupled with nanoAcquity UPLC system (Waters) for exceptional separation. The Orbitrap offers high resolution.

Qtrap 5500 MS (ABSciex) coupled with nanoUPLC Aquity (Waters)
This triple quadrupole mass spectrometer with full MS3 capabilities offers high quality, confidence and sensitivity for targeted quantification studies.

maXis UHR-TOF (Bruker Daltonics) coupled with nanoUPLC Aquity (Waters)
High-resolution tandem mass spectrometer for intact protein detection as well as H/D exchange.

Ultraflex MALDI-TOF/TOF (Bruker Daltonics)
The Ultraflex MALDI (matrix assisted laser desorption/ionization) tandem mass spectrometer is designed for identification of protein spots separated by 2D gel electrophoresis.

QSTAR PULSAR ESI-Qtof MS (Applied Biosystems)
This system is a high performance hybrid quadrupole time of flight mass spectrometer ideal for protein mass determination.


Additional equipment

1D and 2D gels (Invitrogen)
Classic protein analysis methods for separating and visualizing proteins.

Pipetting robot Digest Pro (Intavis)
Automated procedure for routine enzymatic digestion of proteins immobilized in acrylamide gel slices.
Ultimate 3000 RSLC Offline LC System equiped with an automated fraction collector (Thermo)
Offline-LC used for the fractionation of complex protein digests.
Capillary laser puller P-2000F (Sutter Instruments)
Capillary puller for the production of nanoHPLC columns.



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