Ruprecht-Karls-Universität Heidelberg

Contact us:
Zentrum fr Molekulare Biologie der Universitt Heidelberg (ZMBH)
Im Neuenheimer Feld 282
Room 401
69120 Heidelberg, Germany
Tel.: +49-6221-54 6813
Fax: +49-6221-54 5894

Sample preparation

The following sample preparation steps are available in our facility. On request, we also offer users the possibility to perform more elaborate sample preparations within our laboratory on their own.

  • 1D and 2D SDS-PAGE (mini gel format) incl. Coomassie staining
  • Protein digestion
  • Automated sample reduction, alkylation and digestion using an Intavis Digest Pro robot (only gel-based)
  • Sample desalting (C18-based)
    • Online using a trapping column within the LC-MS setup
    • Offline
  • Dimethyl labeling (chemical stabel-isotope labeling) for quantitative proteomics
  • Additional sample fractionation steps - small (StageTip format) or large scale (Offline LC format)
    • SCX
    • SAX
    • high-pH
    • SEC

Intavis Digest Pro robot

Gelbands are excised manualy