Ruprecht-Karls-Universität Heidelberg

Daniela Duarte Campos
ZMBH Research Group Leader

ZMBH
Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Tel. +49 (0) 6221 - 54 ...
Fax +49 (0) 6221 - 54 ...
d.campos@zmbh.uni-heidelberg.de



Welcome to the Duarte Campos lab!

 



Bioprinting for tissue and organ engineering


Our lab investigates biofabrication technologies and biomaterials (i.e. bioinks) suitable for tissue and organ engineering, and their impact on the structure and function of natural and synthetic living tissues.

In bioprinting, there are different types of printing strategies: nozzle-based, for example, extrusion and inkjet printing, and nozzle-free, such as laser induced forward transfer and volumetric printing. During the printing process, cells are exposed to shear stress, which is commonly observed in nozzle-based bioprinting. These can damage not only the cell membrane, but also impact cell structure and function at the longer term. Besides the impact of shear stress on cell function, the type of bioink and its polymerization mechanism, including the use of UV light, can initiate a change in cell phenotype that is harmful and potentially carcinogenic for cells. In this context, the impact of bioprinting technologies at the molecular level, such as DNA damage, endoplasmic reticulum stress, and cell division are the main interests of our group. In addition, we are exploring the development of synthetic biomimetic systems to discover new possible applications in place of biological cells.

 

 

Selected publications

2020

Duarte Campos DF*, Lindsay CD, Roth JG, LeSavage BL, Seymour AJ, Krajina BA, Ribeiro R, Costa P, Blaeser A, Heilshorn SC*. Bioprinting cell- and spheroid-loaded protein-engineered hydrogels as tissue-on-chip platforms. Front Bioeng Biotechnol 2020, doi.org/10.3389/fbioe.2020.00374

Duarte Campos DF*, De Laporte L*. Digitally fabricated and naturally augmented in vitro tissues. Adv Healthc Mater 2020, doi.org/10.1002/adhm.202001253

2019

Blaeser A, Heilshorn S, Duarte Campos DF*. Smart bioinks as de novo building blocks to bioengineer   living tissues. Gels 2019, doi.org/10.3390/gels5020029

Duarte Campos DF*, Zhang S, Kreimendahl F, Köpf M, Fischer H, Vogt M, Blaeser A, Apel C, Esteves-Oliveira M. Hand-held bioprinting for de novo vascular formation applicable to dental pulp regeneration. Connective Tissue Research 2019, doi: 10.1080/03008207.2019.1640217

Duarte Campos DF*, Rohde M, Ross M, Anvari P, Blaeser A, Vogt M, Panfil C, Yam GHF, Mehta JS, Fischer H, Walter P, Fuest M. Corneal bioprinting utilizing collagen-based bioinks and primary human keratocytes. J Biomed Mat Res Part A 2019, doi.org/10.1002/jbm.a.36702

2018

Betsch M, Cristian C, Lin YY, Blaeser A, Schöneberg J, Vogt M, Buhl E, Fischer H, Duarte Campos DF*. Incorporating 4D into bioprinting: Real-time magnetically directed collagen fiber alignment for generating complex multilayered tissues. Adv Healthc Mater 2018, doi.org/10.1002/adhm.201800894


* corresponding author