Open Positions in the Schiebel Lab
The nuclear pore complex (NPC) is a large, cylindrical structure composed of multiple copies of over 30 different proteins known as nucleoporins (NUPs). Embedded in the nuclear envelope at the fusion sites between the inner and outer nuclear membranes, the NPC facilitates the transport of RNA and proteins between the nucleus and the cytoplasm. Malfunctions in NPCs play critical roles in aging and cancer, making the assembly and maintenance of NPCs a topic of utmost importance. During interphase in human cells, or in yeast cells undergoing closed mitosis, NPCs assemble within the intact nuclear envelope through an inside-out mechanism. In this process, NUPs are deposited from within the nucleus onto the inner nuclear membrane, causing deformation that eventually leads to the fusion of the inner and outer nuclear membranes. Recently, we identified two conserved integral membrane proteins, Brr6 and Brl1, which may play a critical role in nuclear membrane fusion during NPC biogenesis. This project will analyse the role of these proteins in the assembly of NPCs in yeast and human cells. The PhD student will employ a variety of techniques, including biochemical approaches, lipid analysis, super-resolution microscopy (MinFlux), electron microscopy, CRISPR/Cas9 technology, and live-cell imaging to study NPC assembly. We are seeking highly motivated PhD students with a background in biochemistry, cell biology or molecular biology. Successful applicants will join an international team of PhD students and postdocs working at the cutting edge of scientific research. The PhD student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (http://www.hbigs.uni-heidelberg.de/).
PhD position " Nuclear pore complex biogenesis"
The PhD position is initially funded for 3 years with starting date based on mutual agreement.
Please send applications to: E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de)
Relevant recent publications:
1 Zhang, W. et al. Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis. J Cell Biol 217, 877-894 (2018).
2 Zhang, W. et al. A short perinuclear amphipathic alpha-helix in Apq12 promotes nuclear pore complex biogenesis. Open biology doi: 10.1098/rsob.210250. (2021).
3 Vitale, J., Khan, A., Neuner, A. & Schiebel, E. A perinuclear alpha-helix with amphipathic features in Brl1 promotes NPC assembly. Mol Biol Cell 33, ar35 (2022). https://doi.org:10.1091/mbc.E21-12-0616
PhD position " Centriole biogenesis"
Are you passionate about cell biology and eager to explore the fascinating world of centrioles?
The Centre for Molecular Biology (ZMBH) at Heidelberg University, under the supervision of Prof. Elmar Schiebel, invites applications for a PhD position focusing on centriole biogenesis.
About the Position:
The successful candidate will join Prof. Elmar Schiebel’s research group, renowned for its groundbreaking work on centrioles and centrosome function. This PhD project will investigate the molecular mechanisms underlying centriole biogenesis, with a focus on understanding how centrioles duplicate and organize microtubules. The research will combine advanced microscopy techniques, biochemistry, and genetic tools to unravel the complexities of centriole assembly and function.
Key Responsibilities:
• Conduct cutting-edge research on centriole biogenesis and its regulation.
• Utilize advanced microscopy and imaging techniques to analyze centriole structure and dynamics.
• Perform biochemical assays including insect cell expression and genetic experiments to investigate centriole-associated proteins.
• Collaborate with a team of researchers and present findings at conferences and in scientific publications. Qualifications:
• A Master’s degree or equivalent in Cell Biology, Molecular Biology, Biochemistry, or a related field. • Strong background in cell and molecular biology techniques.
• Experience with microscopy or biochemistry techniques is advantageous.
• Excellent communication skills and the ability to work independently and as part of a team.
Why Join Us?
• Be part of a vibrant research community at ZMBH, one of the leading institutes for molecular biology.
• Work under the mentorship of Prof. Elmar Schiebel, a leading expert in centriole biology.
• Access state-of-the-art facilities and resources in Heidelberg, a city renowned for its scientific excellence.
• Contribute to impactful research that advances our understanding of fundamental cellular processes.
How to Apply:
Interested candidates should send their application, including a CV, cover letter, and contact information for two references, to Prof. Elmar Schiebel at office-schiebel@zmbh.uni-heidelberg.de
The PhD student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (http://www.hbigs.uni-heidelberg.de/).
The PhD position is funded in the first instance for 3 years with starting date based on mutual agreement. The remuneration is based on TV-L E13.
Relevant recent publications:
1 Liu, P. et al. Insights into the assembly and activation of the microtubule nucleator gamma-TuRC. Nature 578, 467-471 (2020). https://doi.org:10.1038/s41586-019-1896-6
2 Würtz, M. et al. Modular assembly of the principal microtubule nucleator gamma-TuRC. Nat Comm 473, doi: 10.1038/s41467-41022-28079-41460 (2022).
3 Karasu, O. R., Neuner, A., Atorino, E. S., Pereira, G. & Schiebel, E. The central scaffold protein CEP350 coordinates centriole length, stability, and maturation. J Cell Biol 221 (2022). https://doi.org:10.1083/jcb.202203081
4 Gao, Q. et al. The structure of the gamma-TuRC at the microtubule minus end - not just one solution. Bioessays, e2400117 (2024). https://doi.org:10.1002/bies.202400117
5 Vermeulen, B. J. et al. gamma-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end. EMBO J (2024). https://doi.org:10.1038/s44318-024-00087-4