Ruprecht-Karls-Universität Heidelberg

Contact us:
Zentrum fr Molekulare Biologie der Universitt Heidelberg (ZMBH)
Im Neuenheimer Feld 282
Room 401
69120 Heidelberg, Germany
Tel.: +49-6221-54 6813
Fax: +49-6221-54 5894

Logo ZMBH Welcome to the Core Facility for Mass Spectrometry & Proteomics (CFMP) at the ZMBH

Our mission is to provide researchers of the Heidelberg Life Science Campus state-of-the-art high-performance mass spectrometry services. These services include all aspects of protein characterization and qualitative and quantitative proteomics:

- Protein identification from 1D and 2D-gels
- Quantification based on label-free (spectral counting, peptide intensities, MaxQuant-LFQ) and isotope labeling (SILAC & reductive dimethyl labeling) techniques 
- Detection and localization of posttranslational modifications (PTMs)
- Deciphering the interactome of target proteins

Established procedures allow the exploratory analysis of the protein content of cells and organisms and their temporal qualitative and quantitative changes that accompany development, reaction to environmental impacts and pathophysiological processes. Targeted proteomics strategies of high accuracy and sensitivity, such as multiple reaction monitoring (MRM), have been set up for quantitative analysis of known protein networks. Furthermore, the conformation of proteins can be analyzed using amide hydrogen exchange (HDX) mass spectrometry, which allows the analysis of protein mechanics, protein-protein interaction interfaces and ligand-induced allosteric conformational changes.

>> Pictures for the Open House Presentation


22.01.2016 - Announcement - Mass spectrometry workshops 7. - 11.11.2016
From November 7 th - 11 th 2016 we will again offer workshops for our customers, which should enable them to better understand and perform analyses of proteomics datasets. The workshops are each 2 - 3 h long and cover the following topics:
1. Mo 7.11.16  - Identification of interaction partners by spectral counting
2. Tu 8.11.16 - Analysis of posttranslational modifications
3. We 9.11.16 - Identification of regulated proteins by stable-isotope labeling (SILAC)
(incl. hands-on data analysis with Perseus)
4. Th 10.11.16 - Analysis of complex samples using label-free quantification
(incl. hands-on data analysis with Perseus)
5. Fr 11.11.16 - Targeted Proteomics (incl. hands-on Skyline software package)

Register by sending an email naming the course(s) you are interested in at .

16.11.2015 - The new Quadrupol-Orbitrap hybrid mass spectrometer is here!

We are happy to anounce that an additional high resolution hybrid Orbitrap MS system has been added to our portfolio recently. While this brand new Q-Exactive HF instrument will expand our analyses capabilities, it will also allows us to implement new analysis strategies, like data-independent acquisition (DIA) and parallel reaction monitoring (PRM).

Financial Support

ZMBH, DKFZ-ZMBH-Alliance, CellNetworks, HMLS, DKFZ, SFB1036, SFB 638, Graduiertenkolleg 1188, Transregio 83.