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Scientific Job Openings

 
Listed 27.2.2017  

PhD position: Role of human CDC14 in cancer development
Centre for Molecular Biology (ZMBH)
Heidelberg University

CDC14 encodes a highly conserved phosphatase that is essential for cell cycle regulation in the model organism budding yeast. Human cells have two CDC14 paralogues, named hCDC14A and hCDC14B. Very surprisingly, the double knockout of hCDC14A and hCDC14B in human cells does not affect viability and cell cycle progression raising the question of their functions. Recently we could show that hCDC14A is associated with the actin cytoskeleton where it regulates cell adhesion and cell migration. In addition, genomic knockout of hCDC14A makes human colon cancer cells more invasive than the HCT116 control cells. This fits with the observation that downregulation of hCDC14A expression during cancer development is associated with poor survival of patients. In this project we will identify substrates of hCDC14A and hCDC14B by comparing the phospho-proteome profiles of hCDC14A/B knockouts with wild type cells by SILAC/mass spectrometry analysis. In addition, we will identify proximity interactors of hCDC14A/B using the newly developed BioID approach. hCDC14A substrates will be analysed for their role in cell migration and adhesion using the CRISPR/Cas9 knockout strategy. Since hCDC14B associates with the nucleolus, we expect that hCDC14B has different functions and substrates than hCDC14A.
Highly motivated PhD students with a background in biochemistry, cell biology or molecular biology should apply. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research (http://www.cell.com/developmental-cell/meet-the-author/berati-cerikan). The PhD student will be a member of the Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (http://www.hbigs.uni-heidelberg.de/). The PhD position (E13/65) is funded for 3 years.
Please send applications to E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).

Relevant publications:1-5

1          Chen, N. P., Uddin, B., Voit, R. & Schiebel, E. Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion. Proc Natl Acad Sci U S A 113, 990-995, (2016).
2          Mocciaro, A. et al. Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair. J Cell Biol 189, 631-639, (2010).
3          Mocciaro, A. & Schiebel, E. Cdc14: a highly conserved family of phosphatases with non-conserved functions? J Cell Sci 123, 2867-2876, (2010).
4          Pereira, G. & Schiebel, E. Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science 302, 2120-2124, (2003).
5          Agircan, F. G., Hata, S., Nussbaum-Krammer, C., Atorino, E. & Schiebel, E. Proximity mapping of human separase by the BioID approach. Biochem Biophys Res Commun, (2016).

Reviews:
Mocciaro, A. & Schiebel, E. Cdc14: a highly conserved family of phosphatases with non-conserved functions? J Cell Sci 123, 2867-2876, (2010).

 
Listed 29.3.16  

1) Postdoctoral positions are available in the Joazeiro laboratory.

Research in the laboratory investigates the function of E3 ubiquitin ligases in biology and disease (Deshaies & Joazeiro, 2009. Annu Rev Biochem. 78:399-434). The positions available are to elucidate the mechanism of action of the Listerin/Ltn1 E3 ligase in ribosome-associated protein quality control, and/or to understand how defects in Listerin function cause neurodegeneration.

We had previously reported on a new mouse model of ALS/motor neuron disease caused by mutation of Listerin, whose function was unknown at the time (Chu et al. 2009. PNAS 106:2097-103). Listerin is conserved in eukaryotes, so we utilized S. cerevisiae to study its function and found that this E3 is associated with the large ribosomal subunit and mediates quality control of aberrant nascent polypeptides (Bengtson & Joazeiro 2010. Nature 467:470-3; Lyumkis et al 2014. PNAS 111:15981-6).

Our research team undertakes different approaches to study the Listerin pathway, including biochemistry, yeast genetics, structural biology, mammalian tissue culture, and gene-disease association studies using ALS patient samples. We are also well positioned to uncover novel molecular mechanisms underlying neurodegeneration by applying the discoveries we make with these approaches to studies involving the Listerin-mutant mouse model.

The candidate is expected to be proactive and productive, and should have strong background in biochemistry and molecular biology.

Please send CV, a paragraph on current and future research interests, and the names and contact information of three references to Claudio Joazeiro (c.joazeiro@zmbh.uni-heidelberg.de).

 
Listed 20.3.17  
 

The ZENTRUM FÜR MOLEKULARE BIOLOGIE (ZMBH) at Heidelberg University (Germany) is an internationally recognized research center for fundamental research in molecular and cell biology located in one of the largest German campuses for the life sciences. We invite applications from scientists for a

JUNIOR GROUP LEADER POSITION
IN SINGLE PARTICLE ANALYSIS OF MACROMOLECULAR PROTEIN ASSEMBLIES by CRYO ELECTRON MICROSCOPY

The successful candidate will establish an independent research group at the ZMBH for five years with the possibility of extension for up to six additional years. It is expected that his/her research program will extend and complement the spectrum of research topics of the ZMBH and the local community, such as cellular quality control and damage response mechanisms (http://www.zmbh.uni-heidelberg.de/sfb1036/) or spatiotemporal regulation of mitosis. The group will have access to a Krios EM with DED and additional TEM equipment.

The holder of the position will receive a start-up package and institutional support. He/she is expected to also attract external funding and to participate in the department's teaching program in the Faculty of Biosciences (in German or English).

The ZMBH houses 15 independent research groups. We offer an excellent and international research environment, which is further complemented by numerous university institutes and research centers. There are several in-house scientific core facilities, i.e. protein analytics including mass spectrometry, advanced light microscopy, flow cytometry, moreover mechanical and electrical workshops and centralized administrative services are available. Additional nearby facilities on campus include the EM Core Facility. Our Alliance with the German Cancer Research Center (DKFZ-ZMBH Alliance) provides scientific interactions and access to central facilities and scientific infrastructure of the DKFZ.

More information on the research and teaching programs as well as on the scientific facilities can be found on our website (http://www.zmbh.uni-heidelberg.de).
The University of Heidelberg has a policy of raising the proportion of women in academic positions and therefore specifically invites the application of qualified female scientists.
Under German law disabled applicants with full qualifications are to be preferred.

Further details can be obtained from Prof. Dr. Bernd Bukau, telephone +49-6221-546850, telefax: +49-6221-545507. Applications should be sent to the Director of the ZMBH, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany (e-mail: direktor@zmbh.uni-heidelberg.de) by April 30, 2017.

Also published on naturejobs: http://www.nature.com/naturejobs/science/jobs/609327-junior-group-leader-position-in-single-particle-analysis-of-macromolecular-protein-assemblies-by-cryo-electron-microscopy

   

 



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