ZMBH - Imaging Facility

Microscope systems and current configurations (updated 11/2009) Confocal microscopes (1-3) Widefield microscopes (4-10) Stereomicroscopes & macroscopes (11-13)

Confocal microscopes:
1. Leica TCS SP2 confocal microscope Microscope stand: Leica DMIRE2, inverted microscope Laser: 405 nm pulsed diode laser, 1mW 458 nm, 476 nm, 488 nm, 514 nm Argon laser 561 nm DPSS laser, 10 mW 633 nm HeNe laser, 10 mW Objectives: HCX PL APO 63x/1,40-0,60 Oil Lambda BL HCX PL APO 63x/1,20 W CORR Lambda BL HCX Plan APO 40x/0,85 CORR Air HC PL APO 20x/0,70 IMM CORR CS HC PL FLUOAR 20x/0,50 Air PL APO 100x/1,40-0,7 Oil Software: Leica Confocal Software (LCS) Room -132		2. Leica TCS NT confocal microscope Microscope stand: Leica DMIRBE, inverted microscope Laser: 458 nm, 476 nm, 488 nm, 514 nm Argon laser, <10 mW 543 nm HeNe laser, 1.2 mW 633 nm HeNe laser, 10 mW Objectives: HC PL APO 10x/0,40 IMM CS N PLAN L 20x/0,40 CORR/ DIC C HCX PL APO CS 40x/ 0,85 CORR/ DIC C HCX PL APO 40x/ 1,25-0,75 OIL/ DIC E HCX PL APO 63x/ 1,32-0,60 OIL/ DIC D Software: Leica Confocal Software (LCS) Room 355

3. PerkinElmer UltraVIEW ERS Spinning Disk microscope Microscope stand: Zeiss Axiovert 200M, inverted microscope Confocal Scanner Unit: Yokogawa CSU 22 Laser: 405 nm, 440 nm, 640 nm diode laser 488 nm, 514 nm Argon laser 568 nm Krypton laser Objectives: EC Plan-NEOFLUAR 20x/0,5 Air EC Plan-NEOFLUAR 40x/1,3 Oil Plan-APOCHROMAT 63x/1,4 Oil DIC Plan-APOCHROMAT 100x/1,4 Oil Camera: Hamamatsu EMCCD (C9100-50) Software: UltraVIEW ERS Imaging Suite Incubation chamber XL & temperature control unit Room -132



Widefield microscopses:
4. Olympus CellR PointFRAP microscope Microscope stand: Olympus IX81, inverted microscope Laser: 405 nm diode laser, 100 mW 488 nm diode laser, 100 mW Objectives: UPLSAPO 10x/0,4 Air UPLSAPO 40x/0,95 Air DIC UPLSAPO 60x/1,35 Oil PLAPO 100x/1,45 Oil DIC Filters: Ex BP 390/18, Em BP 460/60 (DAPI) Ex BP 434/17, Em BP 479/40 (CFP) Ex BP 469/35, Em BP 525/39 (GFP) Ex BP 497/16, Em BP 535/22 (YFP) Ex BP 562/40, Em BP 641/75 (mCherry) Ex TripleBP 370/36, 474/23, 585/29, Em BP 425/50, 527/50, LP610 (DAPI/GFP/mCherry) Camera: Hamamatsu ORCA-R2 Software: Olympus Cell® Okolab microscope stage incubation system, Lauda low-temp thermostats Room -144		5. Leica DMIRE2, inverted microscope Objectives: N PLAN 10x/0.22 PH1 Air N PLAN L 20x/0,40 CORR Ph1 N PLAN L 40x/0.55 CORR PH2 Air PL APO 40x/1.25-0.75 Oil HCX PL APO 63x/1.40-0.60 Oil HCX PL APO 100x/1.40-0.70 Oil CS Filters: Ex 470/40, Em 525/50 Ex BP 436/20, Em BP 480/40 Ex BP 510/20, Em BP 560/40 Ex BP 510/40, Em LP 610 Camera: Hamamatsu ORCA-ER (C4742-80-12AG) Software: OpenLab 5.0.2 Incubation chamber XL & temperature control unit Room -144

6. Leica DMRXA, upright microscope Objectives: HC PL Fluotar 5x/0.12 PH0 Air HC PL Fluotar 10x/0.30 PH1 Air HC PL Fluotar 20x/0.50 PH2 Air PL Fluotar 40x/0.70 PH2 Air PL APO 40x/1.25-0.75 Oil PL APO 63x/1.0 Oil W CORR PL APO 100x/1.40-0.7 Oil Filters: Ex 340-380, Em LP 425 Ex BP 450-490, Em LP 515 Ex 470/40, Em BP 525/50 Ex 470/40, Em LP 500 Ex BP 515-560, Em LP 590 Ex 531-560, Em BP 574-648 Ex BP 545/30, Em BP 610/75 Ex BP 560/40, Em BP 645/75 Camera: Hamamatsu ORCA-ER (C4742-95-12ER) Software: OpenLab 4.0.2 Room -144		7. Leica DMIRB, inverted microscope Objectives: N Plan 5x/0.11 PH0 Air N Plan 10x/0.22 PH1 Air N Plan L 20x/0.40 Air HC PL APO 20x/0,70 IMM CORR CS Air N PLAN L 40x/0,55 CORR Ph2 Air PL FLUOTAR L 63x/0,70 CORR Ph2 Air Filters: Ex 565/30, Em 620/60 Ex BP 340-380, Em LP 425 Ex BP 450-490, Em LP 515 Ex 500/20, Em 535/30 Camera: Hamamatsu ORCA-100 (C4742-95) Software: OpenLab 4.0.2 Room -144

8. Leica DMRXE, upright microscope Objectives: PL Fluotar 2.5x/0.07 Air PL Fluotar 10x/0.30 Air PH1 PL Fluotar 20x/0.50 Air PH2 PL Fluotar 40x/0.70 Air PH2 PL Fluotar 100x/1.30 Oil PH3 Filters: Ex BP 340-380, Em LP 425 Ex BP 400/20, Em BP 465/20 Ex BP 450-490, Em BP 515-560 Ex 495/15, Em 530/30 Ex 530-595 , Em LP 615 Ex TripleBP 400/20, 495/16, 570/50, Em TripleBP 465/20, 530/30, 640/40 Camera: Princeton Instruments MicroMAX:1300YHS Software: IPLab for Macintosh version 3.2 Room -144		9. Zeiss Axiophot, upright microscope Objectives: Plan NEOFLUAR 10x/0.30 Air Plan NEOFLUAR 20x/0.50 Air Plan NEOFLUAR 40x/0.75 Air Plan-APOCHROMAT 63x/1,40 Oil Plan NEOFLUAR 100x/1.30 Oil Filters: Ex G 365, Em LP 420 Ex BP 546, Em LP 590 Ex 450-490, Em LP 520 Camera: Olympus DP50-CU Software: Studio Lite 1.0 Room -144

10. Zeiss Axiovert 10, inverted microscope Objectives: ACHROSTIGMAT 5x/0,12 Air ACHROSTIGMAT 10x/0,25 Air ACHROSTIGMAT 20x/0,30 Air Ph1 ACHROSTIGMAT 32x/0,40 Air Ph1 Plan NEOFLUAR 40x/0,75 Air Filters: F41-017 HQ-Set EGFP F41-021 HQ-Set Cy 3,5 Camera: ImagingSource DMK2002 Room -144



Stereomicroscopes & macroscopes
11. Leica MZ FLIII fluorescence stereomicroscope Filters: GFP2, CFP, UV, G(reen) Cold light source: Leica KL 1500405 nm pulsed diode laser, 1mW 458 nm, 476 nm, 488 nm, 514 nm Argon laser 561 nm DPSS laser, 10 mW 633 nm HeNe laser, 10 mW Fluorescence light source: Leica HXP 120W Camera: Olympus DP50-CU (optional) Software: Viewfinder Lite version 1.0 Room -144		12. Leica M420 macroscope Cold light sources: Leica KL 1500 LCD & Zeiss KL 2500 LCD Camera: Olympus DP50-CU (optional) Software: Viewfinder Lite version 1.0 Room -144

13. Leica Wild M3Z stereomicroscope Room -144



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ZMBH - Central Services - Imaging Facility - Equipment

Microscope systems and current configurations (updated 11/2009)

Confocal microscopes:

1. Leica TCS SP2 confocal microscope

2. Leica TCS NT confocal microscope

3. PerkinElmer UltraVIEW ERS Spinning Disk microscope

Widefield microscopses:

4. Olympus CellR PointFRAP microscope

5. Leica DMIRE2, inverted microscope

6. Leica DMRXA, upright microscope

7. Leica DMIRB, inverted microscope

8. Leica DMRXE, upright microscope

9. Zeiss Axiophot, upright microscope

10. Zeiss Axiovert 10, inverted microscope

Stereomicroscopes & macroscopes

11. Leica MZ FLIII fluorescence stereomicroscope

12. Leica M420 macroscope

13. Leica Wild M3Z stereomicroscope