University of Heidelberg

ZMBH - Central Services - Flow Cytometry & FACS Core Facility (FFCF)

 

Welcome to the Facility


Flow cytometry and FACS (fluorescence activated cell sorting) represent fast and reliable methods for characterizing and isolating certain cell subpopulations based on fluorescent labeling, and can be applied to a great variety of organisms and cell types, including both prokaryotic and eukaryotic cells.
The facility is equipped with state-of-the-art instruments and offers the possibility of flow cytometric cell analysis and cell sorting to members of the ZMBH, the DKFZ-ZMBH Alliance, as well as to any other research group on the campus. We also welcome projects from non-campus scientists.
For analytical measurements a BD FACSCanto and BD FACSCanto II flow cytometer are available. FACSCanto is equipped with a high-throughput sampler for plate-based acquisition. For cell sorting applications we have a BD FACSAria III or a BD FACSMelody.
The FFCF is supported by the ZMBH. Financial support was also provided by Cell Networks, HMLS, SFB 638, SFB 1036 and the GK 1188. Moreover, different research groups have contributed to the present equipment: Nickel lab, Melchior lab, Edgar lab, and Knop lab. Dr. Monika Langlotz runs the FFCF as head of the facility together with Prof. Dr. Michael Knop as responsible PI.










Access to Instruments and Training

 

The facility offers introduction tutorials to flow cytometry and the use of the analytical instruments, as well as support and aid in all questions concerning flow cytometry as a method. Users, who have received the necessary training, can autonomously operate the analyzers. The facility staff operates the cell sorters and provides aid in using the analyzers.
Trained users have access to our online booking system.

To arrange an individual instrument training or to request a service appointment for cell analysis or sorting please send an e-mail to m.langlotz@zmbh.uni-heidelberg.de.

 

Equipment


Flow Cytometers

BD FACSCanto: 3 lasers (405/488/561), 8 colors (2-2-4), high-throughput sampler option (96 or 384 well plates)

BD FACSCanto II: 3 lasers (405/488/633), 8 colors (2-4-2)

 

Cell Sorters

BD FACSAria III: 4 lasers (407/488/561/633), 11 colors (3-2-4-2), tube sorting (2-way, 4-way), plate sorting

BD FACSMelody: 3 lasers (405/488/561), 8 colors (2-2-4), tube sorting (2-way, 4-way), plate sorting


Flow Cytometry Software

The facility offers access to flow cytometry software for data analysis.

 

Contact      
       
       
Dr. Monika Langlotz
(Head)


ZMBH
Flow Cytometry & FACS Core Facility (FFCF)

EG R066 (Office) and R009/R010 (Lab)
Im Neuenheimer Feld 345
69120 Heidelberg

+49 6221 - 546843

m.langlotz@zmbh.uni-heidelberg.de

       
Claudia Helbig
(TA)
 

+49 6221 - 546843

c.hartmann@zmbh.uni-heidelberg.de

       
       

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