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Scientific Job Openings

 

 

Listed: 08.12.2022        
       

Postdoc and PhD student positions (dry/wet lab): De novo gene birth and human evolution

Center for Molecular Biology (ZMBH), Heidelberg University, Germany

Postdoc and PhD student positions (3 years with possible extensions) are available immediately in the evolutionary genomics group of Henrik Kaessmann. Applications will be reviewed until the positions are filled.

We are seeking highly qualified and enthusiastic applicants with a keen interest in evolutionary questions and a talent and passion for experimental molecular/cellular work and/or bioinformatics analyses (i.e., both joint and distinct dry/wet lab projects are possible).

Our lab has been interested in a range of topics related to the origins and evolution of organs in mammals and other vertebrates and the various underlying genomic changes (see “Selected Publications” below). In the framework of our research, we have generated and analyzed comprehensive genomics (e.g., RNA-seq, ATAC-seq) datasets based on samples from our large organ collections. More recently, we have begun to bring the work of our lab to the level of single cells using state-of-the-art single-cell genomics technologies and bioinformatics procedures. We have also started to experimentally assess the evolutionary impact of selected genomic changes.

The positions will be funded by a recently awarded grant from the NOMIS Foundation (https://nomisfoundation.ch/). In the framework of this grant, we seek to unravel the evolutionary impact of a particularly intriguing class of new genes: protein-coding genes that arose “from scratch” (i.e., from previously noncoding DNA sequence) during evolution – so-called de novo genes. Specifically, we will investigate the prevalence and functional roles of de novo genes in organ development across primates and thus shed light on the molecular evolution underlying the unique biology of humans, in particular that of the brain.

The work will rely on the generation and analyses of translatome (ribosome profiling) data across a unique set of prenatal developmental tissue samples, and the experimental characterization of selected de novo genes in state-of-the-art in vitro organoid systems, ex vivo fetal tissue cultures, and in vivo transgenic mouse models. Notably, the work will be carried out in the framework of collaborations with the labs of Barbara Treutlein (ETH, Basel), Gray Camp (Roche, Basel), and Svante Pääbo (MPI, Leipzig).

Experience in evolutionary and/or developmental biology and/or any of the aforementioned experimental techniques/models are a plus. The individual projects will be developed together with the candidates, based on their respective interests and dry/wet lab skills.

Note that people interested in other topics covered in our lab are also highly welcome to apply (funding is available for strong candidates).

In our lab, we attach great importance to a highly collaborative and positive team spirit! Indeed, we have various “mini-teams” of dry and wet lab researchers within the lab, who enthusiastically drive projects forward in remarkable ways. We are also particularly fond of the diverse cultural backgrounds of our lab members, which contribute to a very enriching atmosphere.

The language of our institute is English and its members form a highly international group. The ZMBH is located in Heidelberg, a picturesque international city next to the large Odenwald forest and Neckar river. The city offers a very stimulating, diverse and collaborative research environment, with the European Molecular Biology Laboratory (EMBL), German Cancer Research Center (DKFZ), Heidelberg Institute of Theoretical Studies (HITS), and the Max Planck Institute for Medical Research located in close proximity to the University.

For more information on the group and our institute more generally, please refer to our website at the ZMBH (http://www.zmbh.uni-heidelberg.de/Kaessmann/).

Please submit a CV, statement of research interest, and names of three references to: Henrik Kaessmann (h.kaessmann@zmbh.uni-heidelberg.de).

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Selected publications:

Murat, F., Mbengue, N., Boeg Winge, S., Trefzer, T., Leushkin, E., Sepp, M., Cardoso-Moreira, M., Schmidt, J., Schneider, C., Mößinger, K., Brüning, T., Lamanna, F., Riera Belles, M., Conrad, C., Kondova, I., Bontrop, R., Behr, R., Khaitovich, P., Pääbo, S., Marques-Bonet, T., Grützner, F., Almstrup, K., Heide Schierup, M., Kaessmann, H. (2022) The molecular evolution of spermatogenesis across mammals. Nature (in press)

Sarropoulos, I., Sepp, M., Fromel, R., Leiss, K., Trost, N., Leushkin, E., Okonechnikov, K., Joshi, P., Giere, P., Kutscher, L.M., Cardoso-Moreira, M., Pfister, S.M., and Kaessmann, H. (2021) Developmental and evolutionary dynamics of cis-regulatory elements in mouse cerebellar cells. Science 373: eabg4696.

Mazin, P.V., Khaitovich, P., Cardoso-Moreira, M., and Kaessmann, H. (2021) Alternative splicing during mammalian organ development. Nat. Genet. 53:924-935.

Wang, Z.Y., Leushkin, E., Liechti, A., Ovchinnikova, S., Mossinger, K., Bruning, T., Rummel, C., Grutzner, F., Cardoso-Moreira, M., Janich, P., Gatfield, D., Diagouraga, B., de Massy, B., Gill, M.E., Peters, A.H.F.M., Anders, S., and Kaessmann, H. (2020) Transcriptome and translatome co-evolution in mammals. Nature 588: 642-647.

Cardoso-Moreira M., Halbert, J., Valloton, D., Velten, B., Chen, C., Shao, Y., Liechti, A., Ascencao, K., Rummel, C., Ovchinnikova, S., Mazin, P.V., Xenarios, I., Harshman, K., Mort, M., Cooper, D.N., Sandi, C., Soares, M.J., Ferreira, P.G., Afonso, S., Carneiro, M., Turner, J.M., VandeBerg, J.L., Fallahshahroudi, A., Jensen, P., Behr, R., Lisgo, S., Lindsay, S., Khaitovich, P., Huber, W., Baker, J., Anders, S., Zhang, Y.E., and Kaessmann H. (2019) Gene expression across mammalian organ development. Nature 571: 505-509.

Sarropoulos, I., Marin, R., Cardoso-Moreira, M., and Kaessmann, H. (2019) Developmental dynamics of lncRNAs across mammalian organs and species. Nature 571: 510-514.

Cortez, D., Marin, R., Toledo-Flores, D., Froidevaux, L., Liechti, A., Waters, P.D., Grutzner, F., and Kaessmann, H. (2014) Origins and functional evolution of Y chromosomes across mammals. Nature 508: 488-493.

Necsulea, A., Soumillon, M., Warnefors, M., Liechti, A., Daish, T., Zeller, U., Baker, J.C., Grutzner, F., and Kaessmann, H. (2014) The evolution of lncRNA repertoires and expression patterns in tetrapods. Nature 505: 635-640.

Brawand, D., Soumillon, M., Necsulea, A., Julien, P., Csardi, G., Harrigan, P., Weier, M., Liechti, A., Aximu-Petri, A., Kircher, M., Albert, F.W., Zeller, U., Khaitovich, P., Grutzner, F., Bergmann, S., Nielsen, R., Paabo, S., and Kaessmann, H. (2011) The evolution of gene expression levels in mammalian organs. Nature 478: 343-348.

         
Listed: 03.11.2022        
       

PhD position:
“Microtubule nucleation – the role of actin”

Centre for Molecular Biology (ZMBH)
Heidelberg University

Microtubules are highly dynamic polymers of a/b-tubulin with essential functions in chromosome segregation in mitosis and meiosis, intracellular organization, cell motility and neurogenesis. Microtubules are targets for anticancer agents, for example Paclitaxel. Microtubule malfunction is associated with cancer, infertility and neurological diseases.

Cells use γ-tubulin complexes for the assembly of microtubules from a/b-tubulin subunits in space and time. We have resolved the cryo-EM structure of the large vertebrate γ-tubulin ring complex (γ-TuRC) composed of over 30 subunits (Liu et al., Nature 2019). Unexpectedly, we identified actin as a component of the γ-TuRC embedded within the γ-TuRC by interacting with MZT1, GCP6 and γ-tubulin. Initial experiments indicate that this actin molecule has a role in γ-TuRC dependent microtubule assembly in cells (Würtz et al., Nat Comm 2022).

In this project we aim to understand the role of actin in the γ-TuRC. The PhD student will be using a broad range of techniques such as cryo-electron microscopy, CRISPR/Cas9 technology for genomic knockins, recombinant expression of the γ-TuRC in insect cells and live cell imaging to study microtubule properties.

The highly motivated PhD student should have a strong background in biochemistry and/or cell biology. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research. The PhD student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (http://www.hbigs.uni-heidelberg.de/). The PhD position is funded for 3 years.

Please send applications (CV, motivation letter, two references, bachelor and master transcripts) to E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).

Relevant publications:

Insights into the assembly and activation of the microtubule nucleator gamma-TuRC. P. Liu, E. Zupa, A. Neuner, A. Bohler, J. Loerke, D. Flemming, et al. Nature 2020 Vol. 578 Issue 7795 Pages 467-471.
Modular assembly of the principal microtubule nucleator gamma-TuRC. M. Würtz, E. Zupa, E. S. Atorino, A. Neuner, A. Bohler, A. S. Rahadian, et al. Nat Commun 2022 Vol. 13 Issue 1 Pages 473.
The gamma-tubulin ring complex: Deciphering the molecular organization and assembly mechanism of a major vertebrate microtubule nucleator. A. Bohler, B. J. A. Vermeulen, M. Wurtz, E. Zupa, S. Pfeffer and E. Schiebel. Bioessays 2021.


         
         
Listed: 03.11.2022        
       

PhD position: “How cancer cells survive centrosome amplification and strategies that disturb these survival mechanisms
Centre for Molecular Biology (ZMBH)
Heidelberg University

The centrosome is the main microtubule organizing centre in mammalian cells. As DNA, centrosomes duplicate once per cell cycle. Malfunction of centrosome duplication is a hallmark of cancer cells and recent data suggest that centrosome overamplification contributes to cell transformation. Cells with supernumerary centrosomes cluster centrosomes together to maintain pseudo-bipolarity associated with low-grade aneuploidy and cell survival. HSET, a nonessential minus end-directed motor of the kinesin-14 family, is a centrosome clustering molecule essential for viability of extra centrosome-bearing cancer cells. However, additional mechanisms cooperate with HSET and ensure survival of cells with supernumerary centrosomes. In this project we will analyse such mechanisms and how they mechanistically work together with HSET. This project involves constructing cell lines using CRISPR/Cas9 technology, the use of protein degron technology for the rapid depletion of proteins, live cell image analysis of cells defective in centrosome clustering, and analysis of organoids with supernumerary centrosomes.
Highly motivated PhD students with a background in biochemistry, cell biology or molecular biology should apply. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research. The PhD student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (http://www.hbigs.uni-heidelberg.de/). The PhD position is funded for 3 years.

Please send applications to E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).

Relevant publications:
Emerging roles of centrosome cohesion. H. Dang and E. Schiebel. Open Bio. 2022 Oct;12(10):220229; doi: 10.1098/rsob.220229.
The balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly. S. Hata, A. Pastor Peidro, M. Panic, P. Liu, E. Atorino, C. Funaya, et al. Nat Cell Biol 2019 Vol. 21 Issue 9 Pages 1138-1151
The centrosomal linker and microtubules provide dual levels of spatial coordination of centrosomes. M. Panic,S. Hata, A. Neuner, E. Schiebel. PLoS Genet. 2015 May 22;11(5):e1005243. doi: 10.1371/journal.pgen.1005243. 

         
         
         
Listed: 16.05.2022        
       

PhD student position: Allosteric mechanisms and activity controls of bacterial AAA+ proteases

AAA+ proteins are ring-forming ATP-fueled protein quality machines that play crucial roles in bacterial physiology and virulence. They use mechanical work to thread substrates through their central channel, leading to disassembly or unfolding of cellular targets. Various bacterial AAA+ proteins (e.g. ClpC, ClpE) associate with peptidases (e.g. ClpP) to form bacterial proteasomes. The degradation activities of proteases are crucial for cellular homeostasis and bacterial virulence. On the other hand, protein degradation by AAA+ proteases can be also potentially deleterious. Substrate selection and ATPase activities of AAA+ proteins are therefore tightly controlled to prevent cellular toxicity. This can be achieved by specific partner proteins (adaptors) that provide substrate specificity and strongly increase AAA+ ATPase activity upon substrate transfer. Persistent, adaptor-independent activation of AAA+ proteins is highly toxic to cells and therefore represents a useful anti-bacterial strategy.
We plan to dissect how AAA+ proteases operate and how their ATPase and threading activities are regulated. We will do so by combining diverse biochemical assays with structural analysis of purified protease complexes by e.g. electron microscopy and crosslinking mass spectrometry.

The salary is based on TV-L scale. The position is limited to three years and is available for immediate start.

The candidate should have a solid background in biochemistry. Please submit a CV, a motivation letter, and names of two references to: Axel Mogk (Bukau lab): a.mogk@zmbh.uni-heidelberg.de).

 

         
         
Listed: 12.05.2022        
       

In the laboratory of Prof. Dr. Daniela Duarte Campos at the Center for Molecular Biology (ZMBH) the following position is to be filled with 39,5h/week:

Postdoc – Bioink Development for In Vivo Bioprinting Applications (f/m/d)

 

For participating in a BMBF NanoMatFutur grant focused on the development of a new in vivo bioprinting technology to fabricate human corneal tissue directly at the operating room.

Corneal damage as a result of injury, infection or hereditary corneal defects can cause pain, blurred vision and eventually blindness. When corneal damage and vision problems cannot be corrected with non-invasive treatments such as eye drops, antibiotics and anti-inflammatory medications, corneal transplants are the ultimate treatment option. This project opens a new way of clinical treatment, for which currently only allografts are used. In short, our concept uses the patient's cells, for example stromal keratocytes differentiated from bone marrow cells, and combines them with a hydrogel that is loaded into a bioprinter. After removing damaged tissue, the surgeon will print the cell-hydrogel mixture layer-by-layer on the patient's eye to create a new cornea.

The Bioprinting Lab investigates biofabrication technologies and biomaterials suitable for in vivo and in vitro tissue and organ engineering, and their impact on the structure and function of natural and synthetic living tissues.

Your task will be to develop a new bioink that allows for in vivo bioprinting of corneal tissue. The work will involve a variety of techniques including but not limited to:

  • Design, synthesis and testing of a new bioink
  • Preparation and handling of natural and synthetic hydrogels
  • Rheological and mechanical characterisation of bioinks for in vivo bioprinting

Your profile:

  • PhD degree in Chemistry, Materials Science, Materials Engineering or related disciplines
  • Passionate about tissue and organ engineering
  • Experience in hydrogel synthesis, rheological and mechanical characterization of hydrogels, and/or bioprinting is desirable
  • Experience in photo-crosslinkable hydrogel systems is a plus
  • Excellent achievements and English language skills
  • Motivation to work in an international environment as part of a team

We offer an international and attractive work environment in the Bioprinting Lab at the ZMBH. The salary is based on TV-L scale. This is a full-time (39,5h/week) position limited to three years. The position is available for immediate start.

Applications should be sent to Prof. Daniela Duarte Campos (dcampos@uni-heidelberg.de) in a single PDF-file that includes a letter of motivation, CV, copy of transcripts and name of 2-3 referees. 
 
Closing date for applications: May 23, 2022
 

Heidelberg University stands for equal opportunities and diversity. Qualified female candidates are especially invited to apply. Disabled persons will be given preference if they are equally qualified. Information on the application process and the collection of personal data is available at www.uni-heidelberg.de/stellenmarkt.

 

         
Listed: 27.01.2022        
       

PhD student positions (dry and wet lab): Vertebrate brain origins and evolution

Center for Molecular Biology (ZMBH), Heidelberg University, Germany

Two PhD student positions (3 years with possible extensions) are available immediately in the evolutionary genomics group of Henrik Kaessmann. Applications will be reviewed until the positions are filled.

We are seeking highly qualified and enthusiastic applicants with a Master degree, a keen interest in evolutionary questions, and a talent and passion for experimental molecular/cellular work and/or bioinformatics analyses (i.e., both joint and distinct dry/wet lab projects are possible).

Our lab has been interested in a range of topics related to the origins and evolution of organs in mammals and other vertebrates and the various underlying genomic/molecular changes (see “Selected Publications” below). In the framework of our research, we have generated and analyzed comprehensive genomics (e.g., RNA-seq, ATAC-seq) datasets based on samples from our large organ collections. More recently, we have begun to bring the work of our lab to the level of single cells using state-of-the-art single-cell genomics technologies and bioinformatics procedures.

The PhD student positions will be funded by a recently awarded ERC Advanced Grant (VerteBrain – “The Ancestral Vertebrate Brain and its Cellular Diversification During Evolution”). In the framework of this grant, we seek to unravel the molecular origins and evolution of the vertebrate brain and its constituent substructures and cell types. Possible projects involve the generation and/or comparative/bioinformatics analyses of extensive single-cell transcriptomic, epigenomic, and spatial transcriptomics data for key species representing all major vertebrate lineages, ranging from jawless vertebrates, such as the sea lamprey, to mammals, such as platypus and human. The precise projects will be developed together with the candidates, based on their respective interests and dry/wet lab skills.

In our lab, we attach great importance to a highly collaborative and positive team spirit! Indeed, we have various “mini-teams” of dry and wet lab researchers within the lab, who enthusiastically drive projects forward in remarkable ways. We are also particularly fond of the diverse cultural backgrounds of our lab members, which contribute to a very enriching atmosphere.

For more information on the group and our institute more generally, please refer to our website at the ZMBH (http://www.zmbh.uni-heidelberg.de/Kaessmann/).

Please submit a CV, statement of research interest, and names of three references to: Henrik Kaessmann (h.kaessmann@zmbh.uni-heidelberg.de).

         
Listed: 27.01.2022        
       

PhD position:
“Microtubules – building them at the right time, at the right place”

Centre for Molecular Biology (ZMBH)
Heidelberg University

Microtubules are highly dynamic polymers with essential functions in chromosome segregation in mitosis and meiosis, intracellular organization, cell motility and neurogenesis. Microtubules are targets for drugs that are used in cancer therapy (Paclitaxel and Vinca alkaloids). Microtubule malfunction is associated with cancer, infertility and neurological diseases.

Using gamma-tubulin complexes, cells have developed mechanisms for the assembly of microtubules from tubulin subunits. We just have resolved the high resolution cryo-EM structure of the large vertebrate gamma-tubulin ring complex (gamma-TuRC) composed of over 30 subunits (published in Nature 2019). Unexpectedly, we identified actin as a component of the gamma-TuRC. The activity of the gamma-TuRC is modulated in time and space by accessory factors, for example microtubule polymerases and activators such as the microcephaly protein CDK5RAP2.

In this project we aim to understand the role of actin in the gamma-TuRC, when and where regulatory co-factors play a role, how they impact the structure of the gamma-TuRC and how the activity of these regulators is changed in cancer and adapted in specific cell types, for example neurons.

The PhD student will be using a broad range of techniques such as cryo-electron microscopy, CRISPR/Cas9 technology for genomic knock-ins and knockouts and live cell imaging to study microtubule assembly.
The highly motivated PhD student should have a background in biochemistry or cell biology. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research. The PhD student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (http://www.hbigs.uni-heidelberg.de/). The PhD position is funded for 3 years

Please send applications (CV, motivation letter, two references, bachelor and master transcripts) to E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).

         
Listed: 27.01.2022        
       

Master Projects

in the laboratory of Prof. E. Schiebel, Centre for Molecular Biology (ZMBH), University of Heidelberg, Germany

My laboratory at the ZMBH, University of Heidelberg, is offering Master projects in the following areas:

The structure and function of gamma-tubulin complexes.  Gamma-tubulin complexes assemble microtubules from tubulin subunits. Microtubules have essential functions for chromosome segregation in mitosis and meiosis, fertility, transport process in particularly in neurons. Drugs such as Taxol that target tubulin are broadly used in cancer therapy. Gamma-tubulin complexes regulate the spatial and temporal formation of microtubules. In this project we determine the structure of gamma-tubulin complexes by Cryo-EM. We will also analyse how gamma-tubulin complexes interact with microtubule polymerases.

Centrosome duplication and centriole-centrosome conversion. Centrosomes are the main microtubule organizing centres in human cells. They only duplicate once per cell cycle. Misregulation of centrosome duplication triggers a p53 response and G1 arrest. This safeguard mechanism is bypassed in cancer cells. After duplication in S phase centrosomes convert into mature centrosomes. Here we analyse the molecular mechanism of this conversion process.

Centrosome cohesion. The two centrosomes of a cell are linked together into one unit from G1 until G2 phase. At the beginning of mitosis this linkage is resolved by the kinase NEK2. Here we will study molecular mechanisms of this linkage, the structure of the linker and functional consequences if centrosome cohesion fails.

Nuclear pore complex biogenesis and spindle pole body duplication. Nuclear pore complexes and the yeast spindle pole body (SPB; yeast microtubule organizing centre) are both embedded in the double membrane of the nuclear envelope. Here we aim to understand how large protein assemblies become inserted into the nuclear envelope and how the inner and outer nuclear envelopes fuse during this embedding process.

During the 6-month master project, you will receive a Student Assistant Contract (Hiwi contract).

 Please send applications with a short motivation letter, CV, bachelor transcripts and the name of one referee to Prof. Dr. E. Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).

         
Listed: 27.01.2022        
       

Lab Rotation in the Laboratory of Prof. Elmar Schiebel, ZMBH, Heidelberg University

My laboratory at the ZMBH, Heidelberg University, is offering lab rotations (6-8 weeks) for Master students who are interested in cell biology, molecular biology or biochemistry.

Topics are:

The structure and function of gamma-tubulin complexes.  Gamma-tubulin complexes assemble microtubules from tubulin subunits. Microtubules have essential functions for chromosome segregation in mitosis and meiosis, fertility, transport process in particularly in neurons. Drugs such as Taxol that target tubulin are broadly used in cancer therapy. Gamma-tubulin complexes regulate the spatial and temporal formation of microtubules. In this project we determine the structure of gamma-tubulin complexes by Cryo-EM.

Genomic tagging of genes in human cells. Presently we are establishing and optimizing approaches based on gRNA and Cas9-like enzymes to tag human genes at the 3’ and 5’ end with a toolbox of PCR cassettes (NeonGreen, GFP, mRuby, auxin degron tag). This allows tagging of human genes within 20 days.

Centrosome duplication and centriole-centrosome conversion. Centrosomes are the main microtubule organizing centres in human cells. They only duplicate once per cell cycle. Misregulation of centrosome duplication triggers a p53 response and G1 arrest. This safeguard mechanism is bypassed in cancer cells. After duplication in S phase centrosomes convert into mature centrosome. Here we analyse the molecular mechanism of the conversion process.

Nuclear pore complex biogenesis and spindle pole body duplication. Nuclear pore complexes and the yeast spindle pole body (SPB; yeast microtubule organizing centre) are both embedded in the double membrane of the nuclear envelope. Here we aim to understand how large protein assemblies become inserted into the nuclear envelope and how the inner and outer nuclear envelopes fuse during this embedding process.

Please send applications to E. Schiebel: schiebel.elmar@zmbh.uni-heidelberg.de

         
Listed: 27.01.2022        
       

Project Title: Structure-function analysis of J-domain cochaperones

Project leader: Matthias P. Mayer

Application Deadline: until position is filled

Start of PhD project: as soon as possible

Source of Funding: University of Heidelberg

Project Description:
In the center of this PhD project is the molecular mechanism of the Hsp70 chaperone machinery. Hsp70 chaperones are central hubs of the protein quality control network and work in collaboration with J-domain cochaperones and nucleotide exchange factors to facilitate a wide variety of protein folding processes and so (re)establish protein homeostasis. The Hsp70s are highly dynamic nanomachines that modulate the conformation of their substrate polypeptides by transiently binding to short, mostly hydrophobic stretches. This interaction is regulated by an intricate allosteric mechanism. The J-domain cochaperones target Hsp70 to their polypeptide substrates, and the nucleotide exchange factors regulate the lifetime of the Hsp70-substrate complexes. In vivo, Hsp70 and J-domain cochaperones are modified by posttranslational modifications and the aim of this PhD project will be to analyze the molecular mechanism J-domain proteins in vitro using biochemical and biophysical techniques with purified proteins. Complementary in-vivo-experiments will be carried out in E. coli and mammalian cell culture.

References:
Mayer, M.P. The Hsp70-chaperone machines in bacteria, Front Mol Biosci. (2021) Jun 7;8:694012. doi: 10.3389/fmolb.2021.694012
Mayer, M. P. & Gierasch, L. M. Recent advances in the structural and mechanistic aspects of Hsp70 molecular chaperones. Journal of Biological Chemistry jbc.REV118.002810 (2018). doi:10.1074/jbc.REV118.002810
Kityk, R., Kopp, J. & Mayer, M. P. Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones. Molecular Cell 69, 227–237.e4 (2018).
Kityk, R., Vogel, M., Schlecht, R., Bukau, B. & Mayer, M. P. Pathways of allosteric regulation in Hsp70 chaperones. Nat Commun 6, 8308 (2015).
Mayer, M. P. Hsp70 chaperone dynamics and molecular mechanism. Trends in Biochemical Sciences 38, 507–514 (2013).

Methods that will be used:
Molecular biology methods, Protein purification methods, biochemical assays, fluorescence spectroscopy and mass spectrometry; E. coli and mammalian cell culture.

Profile of candidate’s qualification:
The candidate should have a strong background in biochemistry/biophysics and should be highly motivated to succeed in science. Experience in mammalian cell culture would be an advantage.

Please send this form to the HBIGS office by email to: r.lutz@hbigs.uni-heidelberg.de

         
     


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