University of Heidelberg

ZMBH - Central Services - Imaging Facility - Equipment


Microscope systems and current configurations (updated 04/2021)

Imaging Facility labs / sample preparation rooms

Imaging Facility course & seminar room (room -127)




Confocal microscopes:
1. Zeiss LSM 780 confocal microscope
  • Microscope stand: Zeiss Axio Observer, inverted microscope
  • Laser:
    405 nm pulsed diode laser, 30 mW
    458 nm, 488 nm, 514 nm Argon laser, 25 mW
    561 nm DPSS laser, 20 mW
    633 nm HeNe laser, 5 mW
  • (pulsed) in Tune laser 490-640 nm, 1.5 mW per wavelength
  • Objectives:
    Plan-APOCHROMAT 20x/0,80 Air
    C-APOCHROMAT 40x/1.2 W Korr-UV-VIS-IR
    Plan-APOCHROMAT 40x/1.3 Oil
    Plan-APOCHROMAT 63x/1.4 Oil
    Plan-APOCHROMAT 100x/1.4 Oil
  • Software: Zeiss ZEN 2010
  • Room -132
2. Leica TCS SP5 confocal microscope
  • Microscope stand: Leica DMI 6000 CS Trino, inverted microscope
  • Laser:
    405 nm diode laser, 50 mW
    458 nm, 5 mW, 476 nm, 5mW, 488 nm, 20 mW, 514 nm, 20 mW Argon laser
    561 nm DPSS laser, 20 mW
    633 nm HeNe laser, 10 mW
  • Objectives:
    HC PL APO 10x/0.40 CS Air
    HCX PL APO 20x/0.70 IMM CORR Lbd BL
    HCX Plan APO 40x/1.30 Oil CS
    HCX PL APO 63x/1.20 W CORR CS
    HCX PL APO 63x/1.30 GLYC CORR CS
    HCX PL APO 63x/1.40-0.60 Oil Lbd BL
  • 2 Hybrid GaAsp detectors (HyD) and 3 PMTs
  • Tandem scanning system
  • Incubation chamber w/ Temp & CO2 control (LIS)
  • Software: Leica Application Suite (LAS)
  • AFC (hardware autofocus)
  • Room -132

3. Leica DMi8 Spinning Disk microscope
  • Microscope stand: Leica DMi8, inverted microscope
  • Confocal Scanner Unit: Yokogawa CSU-X1
  • Laser:
    405 nm, diode laser
    488 nm, 50 mW diode laser
    561 nm, 50 mW diode laser
    640 nm, diode laser
  • Objectives:
    HC PL FLUOTAR 25x/0.8 IMM CORR
    HC PL APO 40x/1.3 Oil
    HC PL APO 63x/1.40-0.60 Oil
    HC PL APO 100x/1.4 Oil CS2
  • Cameras:
    Hamamatsu EMCCD ImagEM Enhanced (C9100-13)
    Hamamatsu Orca Flash 4.0 LT (C11440-42U)
  • Adaptive Focus Control (AFC)
  • Software: MetaMorph Advanced Acquisition
  • Incubation chamber XL & Pecon temperature control unit
  • Room -132
4. Leica TCS SP8 STED 3X microscope with FALCON FLIM
  • Microscope stand: Leica DMi8, inverted microscope
  • Laser:
    White light laser (WLL), 470-670 nm, pulsed
  • STED lasers for superresolution:
    592 nm, 1.5 W
    660 nm, 1.5 W
    775 nm, >1.5 W
  • Objectives:
    HC PL FLUOTAR 10x/0.30 Air
    HC PL APO 20x/0.75 IMM CORR Lbd BL
    HCX PL APO 63x/1.40 Oil CS2
    HC PL APO 93x/1.30 GLYC motC STED W
    HC PL APO 100x/1.40 STED White Oil
  • 2 Hybrid GaAsp detectors (HyD) and 2 PMTs
  • gated STED
  • 3D STED 3X
  • Software: Leica Application Suite X (LASX)
  • FALCON for FLIM, FLIM-FRET, TauSTED and Phasor
  • Room -132

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TIRF/ScannerFRAP widefield microscope:
5. Olympus xcellence TIRF/ScannerFRAP
  • Microscope stand: Olympus IX81, inverted microscope
  • Olympus cellTIRF 4-Line Motorized TIRF Combiner
  • TIRF Lasers:
    405 nm, 100 mW
    488 nm, 100 mW
    561 nm, 100 mW
    640 nm, 140 mW
  • Olympus cellFRAP module
  • ScannerFRAP Laser:
    514 nm, 100 mW
  • Objectives:
    UPLSAPO 60x/1.35 Oil DIC
    PLAPO 100x/1.45 Oil DIC
  • MT 20 illumination system:
    150 W Xe or 150 W Hg/Xe arc burner
    Fast filter wheel
  • Filters:
    Dualband CFP/YFP sbx HC filter set
    Dualband GFP/mCherry sbx ET filter set
    Quad DAPI/FITC/Cy3(Texas Red)/Cy5 sbx HC filter set
    TIRF Quad
  • Cameras:
    Olympus XM10
    Hamamatsu ImagEM Enhanced (C9100-13)
  • Software: Olympus xcellence
  • ZDC (hardware autofocus)
  • Incubation chamber w/ Temp and CO2 control units
  • TMC Linos optical table 63-550
  • Room -132

Widefield screening microscope:
6. Olympus ScanR screening/CellSens system
  • Microscope stand: Olympus IX81, inverted microscope
  • Objectives:
    UPLSAPO 20x/0.75 Air
    UPLSAPO 40x/0.95 Air
    UPLFL N 60x/0.90 Air
    PLAPO 100x/1.45 Oil
  • Lumencor Spectra X illumination system
  • Filters for:
    DAPI
    CFP
    GFP
    YFP
    RFP/mCherry
    Cy5
  • Camera: Hamamatsu Orca Flash 4.0
  • Software: Olympus ScanR_2.5.0 & CellSens
  • ZDC (hardware autofocus)
  • Incubation chamber w/ Pecon temp control
  • Room -144

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Widefield microscopes:
7. Olympus CellSens microscope
8. Nikon BioStation IM-Q Time Lapse Imaging System
  • For glass bottom dishes
  • Objectives:
    40x (20x, 40x, 80x by switching tube lens), NA 0.8,
  • Filters:
    Ex 438/24, Em 483/32 CFP/Cerulean
    Ex 472/30, Em 520/35 GFP
    Ex 500/24, Em 542/27 YFP
    Ex 562/40, Em 624/40 Alexa568/mCherry/TxRed
  • Camera: 1.3-megapixel cooled monochrome camera
  • Software: Nikon
  • Room -144

9. Applied Precision (GE) PersonalDV
  • Objectives:
    UPlanSApo 10x/0.40 Air
    UPlanSApo 20x/0.75 Air
    UPlanSApo 40x/0.95 Air
    UPlanSApo 20x/0.85 Oil
    UApo/340 40x/1.35 Iris Oil
    PlanApo N 60x/1.42 Oil
  • Filters:
    Dichroic 1: GFP & RFP
    Ex 490/20, Em 528/38
    Ex 555/28, Em 617/73
    Dichroid 2: CFP & YFP & mCherry
    Triple "Sedat" sbxm ET Filterset
  • Camera: Photometrics CoolSNAP HQ2
  • Software: Resolve3D/softWoRx
  • UltimateFocus (hardware autofocus)
  • Environmental Chamber
  • TMC optical table 63-534
  • Room -144
10. Leica DMRXA, upright microscope
  • Objectives:
    N Plan 5x/0.12 PH0 Air
    HC PL Fluotar 10x/0.30 PH1 Air
    HC PL Fluotar 20x/0.50 PH2 Air
    PL Fluotar 40x/0.70 PH2 Air
    PL APO 40x/1.25-0.75 Oil
    PL APO 63x/1.0 W CORR
    PL APO 100x/1.40-0.7 Oil
  • Filters:
    Ex 340-380, Em LP 425 (DAPI)
    Ex BP 450-490, Em LP 515 (FITC)
    Ex 470/40, Em BP 525/50 (GFP)
    Ex 470/40, Em LP 500 (EGFP)
    Ex BP 620/60, Em LP 700/75 (Cy5)
    Ex 531-560, Em BP 574-648 (TexasRed)
    Ex BP 436/20, Em BP 480/40 (CFP)
    Ex BP 560/40, Em BP 645/75 (Cy3)
  • Camera: Hamamatsu ORCA-ER (C4742-95-12ER)
  • Software: Micro-Manager
  • Room -144

11. Zeiss AxioObserver
  • Microscope stand: Zeiss Axio Observer Z1
  • Objectives:
    LD-Plan-NEOFLUAR 20x/0.4 Air Ph2
    LD-Plan-NEOFLUAR 40x/0.6 Air Ph2
    LD-Plan-NEOFLUAR 40x/1.3 Oil DIC
    Plan-APOCHROMAT 63x/1.4 Oil DIC
    Plan-NEOFLUAR 100x/1.3 Ph3
  • Filters:
    Ex BP 340-380, Em LP 425
    Ex BP 450-490, Em BP 515-560
    Ex 530-595, Em LP 615
    Ex 570/50, Em 640/40
  • Camera: AxioCam MRm
  • Software: AxioVision
  • Colibri LED light source
  • Definite Focus (hardware autofocus)
  • Incubation chamber w/ CO2 ModuleS and TempModuleS
  • Room -144

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Micromanipulation/-injection system:
12. AIS 2 micromanipulation system
  • Microscope stand: Zeiss Axiovert 10, inverted microscope
  • Injection system: Luigs & Neumann, Germany
  • Injection pressure system: Eppendorf FemtoJet
  • Objectives:
    ACHROSTIGMAT 5x/0.12 Air
    ACHROSTIGMAT 10x/0.25 Air
    ACHROSTIGMAT 20x/0.30 Air Ph1
    ACHROSTIGMAT 32x/0.40 Air Ph1
    Plan NEOFLUAR 40x/0.75 Air
  • Filters:
    F41-017 HQ-Set EGFP
    F41-021 HQ-Set Cy 3,5
  • Camera: ImagingSource DMK2002
  • Software: AIS 2 control program
  • Room -144

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Stereomicroscopes & macroscopes:
13. Leica MZ FLIII fluorescence stereomicroscope
  • Filters: GFP2, CFP, UV, G(reen) ex.
  • Cold light source: Leica KL 1500 LCD
  • Fluorescence light source: Leica HXP 120W
  • Camera: ProgRes C10plus (optional)
  • Software: Micro-Manager
  • Room -144
14. Leica M420 macroscope
  • Cold light sources: Leica KL 1500 LCD & Zeiss KL 2500 LCD
  • Camera: ProgRes C10plus (optional)
  • Software: Micro-Manager
  • Room -144

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Imaging Facility labs / sample preparation rooms:

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